Recombinant Viral B8R Biotinylated Protein, CF

Catalog # BT8225 | R&D Systems, Inc. a Bio-Techne Brand
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BT8225-025
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Key Product Details

Accession #

P24770

Source

CHO

Structure / Form

Noncovalently-linked homodimer, biotinylated via sugars

Conjugate

Biotin

Applications

Bioactivity

Product Specifications

Source

Chinese Hamster Ovary cell line, CHO-derived viral B8R protein
Lys18-Ser272, with a C-terminal 6-His tag

Purity

>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

Lys18

Predicted Molecular Mass

30 kDa (unlabeled)

Activity

Measured by its ability to inhibit rhIFN-gamma mediated protection of HeLa human cervical epithelial carcinoma cells to viral lysis. Meager, A. (1987) in Lymphokines and Interferons, a Practical Approach. Clemens, M.J. et al. (eds): IRL Press. 129.
Th ED50 for this effect is 0.5-3 ng/mL.

Scientific Data Images for Recombinant Viral B8R Biotinylated Protein, CF

Recombinant Viral B8R Biotinylated Protein Bioactivity

Recombinant Viral B8R Biotinylated Protein Bioactivity

Both Biotinylated Recombinant Viral B8R (Catalog # BT8225) and unlabeled Recombinant Viral B8R (Catalog # 8225-BR) inhibits IFN-gamma-mediated protection of HeLa human cervical epithelial carcinoma cells to viral lysis. The ED50 for this effect is 0.5-3 ng/mL. The similarity in activity highlights that the biotinylated protein is fully functional.

Formulation, Preparation and Storage

BT8225
Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution
Reconstitute at 250 μg/mL in PBS.

Reconstitution Buffer Available:
Size / Price
Qty
Shipping The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: B8R

B8R is a secreted Interferon gamma (IFN-gamma) binding protein encoded by the open reading frame of the Vaccina virus (smallpox) (1, 2). B8R is a 43 kDa protein and shares only 19% amino acid sequence identity with the extracellular region of the human IFN-gamma Receptor 1 (IFN-gamma R1). However, B8R binds IFN-gamma orthologs from multiple species including human, rat, rabbit, bovine, and chicken with high affinity, but it binds mouse IFN-gamma with low affinity (1, 3, 4). During infection,
virally-induced B8R binds and sequesters endogenous IFN-gamma, thereby suppressing the host immune response and promoting viral immune evasion (5). While B8R is not required for viral replication, it contributes significantly to Vaccinia virus virulence and is frequently inactivated prior to Vaccinia use in vaccines (5-9). IFN-gamma peptide mimetics that activate IFN-gamma R1 but are not bound and sequestered by B8R have been developed for research and therapeutic use (10, 11).

References

  1. Alcami, A. and G.L. Smith (1995) J. Virol. 69:4633.
  2. Goebel, S.J. et al. (1990) Virology 179:247.
  3. Puehler, F. et al. (1998) Virology 248:231.
  4. Mossman, K. et al. (1995) Virology 208:762.
  5. Smith, G.L. et al. (2013) J. Gen. Virol. 94:2367.
  6. Symons, J.A. et al. (2002) J. Gen. Virol. 83:1953.
  7. Verardi, P.H. et al. (2001) J. Virol. 75:11.
  8. Denes, B. et al. (2006) J. Gene Med. 8:814.
  9. Dimier, J. et al. (2011) J. Virol. 85:5016.
  10. Ahmed, C.M. et al. (2007) J. Immunol. 178:4576.
  11. Ahmed, C.M. et al. (2005) J. Virol. 79:5632.

Long Name

Soluble Interferon alpha/beta Receptor B8

Entrez Gene IDs

3707661 (Viral)

Gene Symbol

B8R

UniProt

P24770

Product Documents for Recombinant Viral B8R Biotinylated Protein, CF

Certificate of Analysis

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Product Specific Notices for Recombinant Viral B8R Biotinylated Protein, CF

For research use only

Reconstitution Calculator

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