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Recombinant SARS-CoV Nucleocapsid His-tag Protein, CF

Bio-Techne includes R&D Systems | Catalog # 10710-CV

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10710-CV-100

Key Product Details

Source

Sf 21 (baculovirus)

Accession #

Conjugate

Unconjugated

Product Specifications

Source

Spodoptera frugiperda, Sf 21 (baculovirus)-derived sars-cov Nucleocapsid protein
Met1-Ala422, with a C-terminal 6-His tag

Purity

>85%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

Ser2, determined by protein ID

Predicted Molecular Mass

47 kDa

SDS-PAGE

41-55 kDa, under reducing conditions

Activity

Bioassay data are not available.

Formulation, Preparation and Storage

10710-CV
Formulation Lyophilized from a 0.2 μm filtered solution in PBS and EDTA with Trehalose.
Reconstitution Reconstitute at 1 mg/mL in PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Nucleocapsid

SARS-CoV was discovered in association with cases of severe acute respiratory syndrome (SARS) that infected more than 8,000 persons with over 900 fatalities worldwide in 2002-2003 (1). It belongs to a family of viruses known as coronaviruses that also include MERS and SARS-Cov2 that causes the global pandemic coronavirus disease 2019 (Covid-19). Coronavirus is commonly comprised of four structural proteins: Spike protein(S), Envelope protein (E), Membrane protein (M), and Nucleocapsid protein (N) (1). While the S, E and M proteins build up the viral envelop, the N protein is involved in transcription, replication, and packaging of the viral RNA genome into a helical ribonucleocapsid (RNP) (2, 3). The SARS-CoV N protein is a ~45 kDa protein composed of two independent structural domains connected by a linker region. Both the N-terminal and the linker regions contain RNA binding domains, while the C-terminal region is responsible for the oligomerization of the N protein (2, 3). The SARS-CoV N protein shares 90% amino acid sequence identity with SARS-CoV-2 N protein. The N protein is an abundant protein during coronavirus infection and displays high immunogenic activity (4, 5), so it has been used to develop serological diagnostic kit for SARS IgM and IgG antibody tests.

References

  1. Rota, P.A. et al. (2003) Science 300:1394.
  2. Chang, C.K. et al. (2006) J.Biomed. Sci. 13:59.
  3. Hurst, K. R. et al. (2009) J. Virol. 83:7221.
  4. Che, X. Y. et al. (2004) J. Clin. Microbiol. 42:2629.
  5. Guan, M. et al. (2004) Clin. Diagn. Lab. Immunol. 11:287.

Long Name

Nucleocapsid Protein

Alternate Names

N Protein

Entrez Gene IDs

918763 (HCoV-229E); 3200423 (HCoV-HKU1); 2943504 (HCoV-NL63); 39105221 (HCoV-OC43); 14254601 (MERS-CoV); 1489678 (SARS-CoV); 43740575 (SARS-CoV-2)

Gene Symbol

N

UniProt

Additional Nucleocapsid Products

Product Documents for Recombinant SARS-CoV Nucleocapsid His-tag Protein, CF

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Note: Certificate of Analysis not available for kit components.

Product Specific Notices for Recombinant SARS-CoV Nucleocapsid His-tag Protein, CF

For research use only

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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