Product Specifications for Recombinant SARS-CoV Nucleocapsid His-tag Protein, CF
Spodoptera frugiperda, Sf 21 (baculovirus)-derived sars-cov Nucleocapsid protein Met1-Ala422, with a C-terminal 6-His tag
>85%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
<0.10 EU per 1 μg of the protein by the LAL method.
N-terminal sequence Analysis
Ser2, determined by protein ID
Predicted Molecular Mass
41-55 kDa, under reducing conditions
Bioassay data are not available.
Formulation, Preparation and Storage
What does CF mean?
CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our
Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant
protein to be stored at a more dilute concentration.
The carrier free version does not contain BSA.
What formulation is right for me?
In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or
as an ELISA standard.
In contrast, the carrier free protein is recommended for applications, in which the presence of BSA
Lyophilized from a 0.2 μm filtered solution in PBS and EDTA with Trehalose.
Reconstitute at 1 mg/mL in PBS.
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage:
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
3 months, -20 to -70 °C under sterile conditions after reconstitution.
SARS-CoV was discovered in association with cases of severe acute respiratory syndrome (SARS) that infected more than 8,000 persons with over 900 fatalities worldwide in 2002-2003 (1). It belongs to a family of viruses known as coronaviruses that also include MERS and SARS-Cov2 that causes the global pandemic coronavirus disease 2019 (Covid-19). Coronavirus is commonly comprised of four structural proteins: Spike protein(S), Envelope protein (E), Membrane protein (M), and Nucleocapsid protein (N) (1). While the S, E and M proteins build up the viral envelop, the N protein is involved in transcription, replication, and packaging of the viral RNA genome into a helical ribonucleocapsid (RNP) (2, 3). The SARS-CoV N protein is a ~45 kDa protein composed of two independent structural domains connected by a linker region. Both the N-terminal and the linker regions contain RNA binding domains, while the C-terminal region is responsible for the oligomerization of the N protein (2, 3). The SARS-CoV N protein shares 90% amino acid sequence identity with SARS-CoV-2 N protein. The N protein is an abundant protein during coronavirus infection and displays high immunogenic activity (4, 5), so it has been used to develop serological diagnostic kit for SARS IgM and IgG antibody tests.
Rota, P.A. et al. (2003) Science 300:1394.
Chang, C.K. et al. (2006) J.Biomed. Sci. 13:59.
Hurst, K. R. et al. (2009) J. Virol. 83:7221.
Che, X. Y. et al. (2004) J. Clin. Microbiol. 42:2629.
Guan, M. et al. (2004) Clin. Diagn. Lab. Immunol. 11:287.
Which Brands are Currently Available on bio‑techne.com? R&D Systems, Tocris Bioscience and ProteinSimple branded products are available to purchase through bio‑techne.com. ProteinSimple branded instruments are available to quote. ACD branded products will be available on bio‑techne.com in the near future. Novus Biologicals branded products are not currently available on bio‑techne.com and can be found at novusbio.com.
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