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Recombinant SARS-CoV 3CL Protease, CF

Bio-Techne includes R&D Systems | Catalog # E-718

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E-718-050

Key Product Details

Source

E. coli

Accession #

Conjugate

Unconjugated

Applications

Bioactivity

Product Specifications

Source

E. coli-derived sars-cov 3CL Protease protein
Ser1 - Gln306 with a C-terminal 6-His tag

Purity

>95%, by SDS-PAGE under reducing conditions and visualized by Colloidal Coomassie® Blue stain.

Predicted Molecular Mass

34 kDa

Activity

Reaction conditions will need to be optimized for each specific application.

Scientific Data Images for Recombinant SARS-CoV 3CL Protease, CF

Recombinant SARS-CoV 3CL Protease Inhibition Activity

Recombinant SARS-CoV 3CL Protease Inhibition Activity

GC376 achieved 50% inhibition of 50 nM SARS-CoV Virus 3CL Protease (Catalog # E-718) at a concentration of 101-120 nM.

SDS-PAGE of Recombinant SARS-CoV Virus 3CL Protease.

1 μg and 2 μg of Recombinant SARS-CoV Virus 3CL Protease (Catalog # E-718) was resolved with SDS-PAGE under reducing conditions and visualized by colloidal Coomassie Blue staining, showing a band at 33 kDa.

Formulation, Preparation and Storage

E-718
Formulation Supplied as a solution in HEPES, NaCl, DTT and EDTA.
Shipping The product is shipped with dry ice or equivalent. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 6 months from date of receipt, -70 °C as supplied.
  • 3 months, -70 °C under sterile conditions after opening.

Background: 3CL Protease

The 3CL protease (aka 3CLpro, Mpro or "Main Protease) from the human SARS-CoV coronavirus (Severe Acute Respiratory Syndrome coronavirus) is a C30-type cysteine protease located within the non-structural proteins 3 (NS3) section of the viral polypeptide.  3CLpro activity is required to process the viral polyprotein into functional, mature subunits, and there are 11 or more sites of cleavage, many containing the sequence LQ[S/A/G]; the protease cleaves c-terminal to the glutamine amino acid.  Along with the CoV Papain-Like Protease (catalog number E-610), 3CLpro presents an attractive target for therapeutic intervention of coronavirus infections.  Because no human proteases with a similar cleavage specificity are known, inhibitors of 3CLpro are unlikely to cause mechanism-based toxicity.  

References

  1. Chen, Y.W., et al. (2020) F1000Research 9:129.
  2. Pillaiyar, T., et al. (2016) J. Med. Chem. 59:6595.

Long Name

3CL Protease

Alternate Names

Main Protease, Mpro

UniProt

Additional 3CL Protease Products

Product Documents for Recombinant SARS-CoV 3CL Protease, CF

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Note: Certificate of Analysis not available for kit components.

Product Specific Notices for Recombinant SARS-CoV 3CL Protease, CF

For research use only

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