Recombinant SARS-CoV-2 Nucleocapsid His Protein, CF

Name: Recombinant SARS-CoV-2 Nucleocapsid His Protein, CF
Brand: R&D Systems
SKU: 10474-CV
Rating: 4 (1 reviews)

Catalog # 10474-CV

(1)

Product Datasheet / COA / SDS

Sf21 Insect Cell Expressed, Over 95% Purity

Order Information

Bio-Techne Corporation

tel: (800) 343-7475 (free phone)

tel: (612) 379-2956

customercare.na@bio-techne.com

100% Guarantee on all
R&D Systems products.

Shipping Information

$35 to United States

Products in stock will be shipped in 1-3 business days unless specified.

View Distributors

Key Product Details

Source:	Sf 21 (baculovirus)
Accession #:	YP_009724397.2
Applications:	Bioactivity

View Full Technical Details for Recombinant SARS-CoV-2 Nucleocapsid His Protein, CF

Product Specifications

Source	Spodoptera frugiperda, Sf 21 (baculovirus)-derived sars-cov-2 Nucleocapsid protein Met1-Ala419, with a C-terminal 6-His tag
Purity	>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Endotoxin level	<0.10 EU per 1 μg of the protein by the LAL method.
N-terminal sequence Analysis	Ser2
Predicted Molecular Mass	46 kDa
SDS-PAGE	44-53 kDa, under reducing conditions
Activity	Bioassay data are not available.

Recombinant SARS-CoV-2 Nucleocapsid His Protein, CF Scientific Data Examples

	Recombinant SARS-CoV-2 Nucleocapsid His-tag Protein SDS-PAGE. 2 μg/lane of Recombinant SARS-CoV-2 Nucleocapsid His Protein (Catalog # 10474-CV) was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by Coomassie® Blue staining, showing bands at 44-53 kDa.

Formulation, Preparation and Storage

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

10474-CV

Formulation	Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution	Reconstitute at 500 μg/mL in PBS.
Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage:	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

Volume (to add to vial)

Mass (in vial)

Desired Reconstitution Concentration

Background: Nucleocapsid

SARS-CoV-2, which causes the global pandemic coronavirus disease 2019 (Covid-19), belongs to a family of viruses known as coronaviruses that are commonly comprised of four structural proteins: Spike protein (S), Envelope protein (E), Membrane protein (M), and Nucleocapsid protein (N) (1). While the S, E and M proteins build up the viral envelop, the N protein is involved transcription, replication and packaging of the viral RNA genome into a helical ribonucleocapsid (RNP) (2, 3). The SARS-CoV-2 N protein is a ~45 kDa protein composed of two independent structural domains connected by a linker region. The N-terminal region contains an RNA binding domain, the linker region interacts with the M protein and the C-terminal region contains a self-association domain (2,3). The SARS-CoV2 N protein shares 91% and 47% amino acid sequence identity with SARS-CoV-1 and MERS N protein, respectively. The SARS-CoV-2 N protein displays VSR (viral suppressor of RNA interference) activity in mammalian cells (4). In addition, the N protein is an abundant protein during coronavirus infection and displays high immunogenic activity (5, 6), so it has been used to develop serological diagnostic kit for Covid-19 IgM and IgG antibody tests (7).

Item	Value
References	Wu, F. et al. (2020) Nature 579:265. Chang, C. K. et al. (2006) J. Biomed. Sci. 13:59. Hurst, K. R. et al. (2009) J. Virol. 83:7221. Mu, J. et al. (2020) Sci. China Life Sci. doi: 10.1007/s11427-020-1692-1. Che, X. Y. et al. (2004) J. Clin. Microbiol. 42:2629. Guan, M. et al. (2004) Clin. Diagn. Lab. Immunol. 11:287. Liu, W. et al. (2020) J. Clin. Microbiol. doi: 10.1128/JCM.00461-20.
Long Name	Nucleocapsid Protein
Entrez Gene IDs	918763 (HCoV-229E); 3200423 (HCoV-HKU1); 2943504 (HCoV-NL63); 39105221 (HCoV-OC43); 14254601 (MERS-CoV); 1489678 (SARS-CoV); 43740575 (SARS-CoV-2)
Alternate Names	N Protein, Nucleocapsid

Citations for Recombinant SARS-CoV-2 Nucleocapsid His Protein, CF

View all citations for the Proteins and Enzymes platform.
Please visit our Instrument Citation Database to search our collection of scientific articles that feature our instruments.

Customer Reviews (1)

4 out of 5

1 customer rating

5 stars

4 stars

100%

3 stars

2 stars

1 star

Customer Images

Showing 1 - 1 of 1 review Showing All

Filter By:

Recombinant SARS-CoV-2 Nucleocapsid His Protein, CF

Name: Anonymous

Application: In vitro bioactivity in cell culture

Reason for Rating: Stimulated immune cells evidenced by increased cytokine expression.

Verified Customer | Posted 06/20/2022

There are no reviews that match your criteria.

Showing 1 - 1 of 1 review Showing All

Product Documents

Product Datasheets

Download Product Datasheets

COA

SDS

Download SDS

Recombinant SARS-CoV-2 Nucleocapsid His Protein, CF

Order Information

Shipping Information

Key Product Details

Complete Your Research

Product Specifications

Recombinant SARS-CoV-2 Nucleocapsid His Protein, CF Scientific Data Examples

Formulation, Preparation and Storage

Carrier Free

Reconstitution Calculator

Background: Nucleocapsid

Citations for Recombinant SARS-CoV-2 Nucleocapsid His Protein, CF

Customer Reviews (1)

Customer Images

Recombinant SARS-CoV-2 Nucleocapsid His Protein, CF

Product Documents

Product Datasheets

COA

Certificate of Analysis

SDS

Bio-Techne Brands

Stay Connected