Recombinant SARS-CoV-2 Nucleocapsid His Biotin Protein, CF

Name: Recombinant SARS-CoV-2 Nucleocapsid His Biotin Protein, CF
Brand: R&D Systems
SKU: BT10474
Price: 636 USD

Catalog # BT10474 | R&D Systems, Inc. a Bio-Techne Brand

Product Datasheet / COA / SDS

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Key Product Details

Source:	Sf 21 (baculovirus)
Accession #:	YP_009724397.2
Structure / Form:	Biotinylated via amines

View Full Technical Details for Recombinant SARS-CoV-2 Nucleocapsid His Biotin Protein, CF

Product Specifications for Recombinant SARS-CoV-2 Nucleocapsid His Biotin Protein, CF

Source	Spodoptera frugiperda, Sf 21 (baculovirus)-derived sars-cov-2 Nucleocapsid protein Met1-Ala419, with a C-terminal 6-His tag
Purity	>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Endotoxin level	<0.10 EU per 1 μg of the protein by the LAL method.
N-terminal sequence Analysis	Ser2
Predicted Molecular Mass	46 kDa
SDS-PAGE	49-59 kDa, under reducing conditions
Activity	Bioassay data are not available.

Scientific Data Examples for Recombinant SARS-CoV-2 Nucleocapsid His Biotin Protein, CF

	2 μg/lane of Biotinylated Recombinant SARS-CoV-2 Nucleocapsid His-tag Protein (Catalog # BT10474) was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by Coomassie® Blue staining, showing bands at 49-59 kDa.

Formulation, Preparation and Storage

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

BT10474

Formulation	Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.
Reconstitution	Reconstitute at 500 μg/mL in PBS.
Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage:	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: Nucleocapsid

SARS-CoV-2, which causes the global pandemic coronavirus disease 2019 (Covid-19), belongs to a family of viruses known as coronaviruses that are commonly comprised of four structural proteins: Spike protein (S), Envelope protein (E), Membrane protein (M), and Nucleocapsid protein (N) (1). While the S, E and M proteins build up the viral envelop, the N protein is involved transcription, replication and packaging of the viral RNA genome into a helical ribonucleocapsid (RNP) (2, 3). The SARS-CoV-2 N protein is a ~45 kDa protein composed of two independent structural domains connected by a linker region. The N-terminal region contains an RNA binding domain, the linker region interacts with the M protein and the C-terminal region contains a self-association domain (2,3). The SARS-CoV2 N protein shares 91% and 47% amino acid sequence identity with SARS-CoV-1 and MERS N protein, respectively. The SARS-CoV-2 N protein displays VSR (viral suppressor of RNA interference) activity in mammalian cells (4). In addition, the N protein is an abundant protein during coronavirus infection and displays high immunogenic activity (5, 6), so it has been used to develop serological diagnostic kit for Covid-19 IgM and IgG antibody tests (7).

Item	Value
References	Wu, F. et al. (2020) Nature 579:265. Chang, C. K. et al. (2006) J. Biomed. Sci. 13:59. Hurst, K. R. et al. (2009) J. Virol. 83:7221. Mu, J. et al. (2020) Sci. China Life Sci. doi: 10.1007/s11427-020-1692-1. Che, X. Y. et al. (2004) J. Clin. Microbiol. 42:2629. Guan, M. et al. (2004) Clin. Diagn. Lab. Immunol. 11:287. Liu, W. et al. (2020) J. Clin. Microbiol. doi: 10.1128/JCM.00461-20.
Long Name	Nucleocapsid Protein
Entrez Gene IDs	918763 (HCoV-229E); 3200423 (HCoV-HKU1); 2943504 (HCoV-NL63); 39105221 (HCoV-OC43); 14254601 (MERS-CoV); 1489678 (SARS-CoV); 43740575 (SARS-CoV-2)
Alternate Names	N Protein, Nucleocapsid

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Recombinant SARS-CoV-2 Nucleocapsid His Biotin Protein, CF

Catalog # BT10474 | R&D Systems, Inc. a Bio-Techne Brand

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Product Specifications for Recombinant SARS-CoV-2 Nucleocapsid His Biotin Protein, CF

Scientific Data Examples for Recombinant SARS-CoV-2 Nucleocapsid His Biotin Protein, CF

Formulation, Preparation and Storage

Carrier Free

Reconstitution Calculator

Background: Nucleocapsid

Citations for Recombinant SARS-CoV-2 Nucleocapsid His Biotin Protein, CF

Product Documents for Recombinant SARS-CoV-2 Nucleocapsid His Biotin Protein, CF

Product Datasheets

COA

Certificate of Analysis

SDS

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