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Recombinant SARS-CoV-2 B.1.617.2 N His-tag Protein, CF

Catalog # 10999-CV | R&D Systems, Inc. a Bio-Techne Brand
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10999-CV-100

Key Product Details

Source

Sf 21 (baculovirus)

Accession #

Conjugate

Unconjugated

Applications

Bioactivity

Product Specifications

Source

Spodoptera frugiperda, Sf 21 (baculovirus)-derived sars-cov-2 Nucleocapsid protein
Met1-Ala419 (Asp63Gly, Arg203Met, Gly215Cys, Asp377Tyr), with a C-terminal 6-His tag

Purity

>90%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

Protein identity confirmed by mass spectrometry.

Predicted Molecular Mass

46 kDa

SDS-PAGE

45-55 kDa, under reducing conditions.

Activity

Bioassay data are not available.

Scientific Data Images for Recombinant SARS-CoV-2 B.1.617.2 N His-tag Protein, CF

Recombinant SARS-CoV-2 B.1.617.2 Nucleocapsid His-tag Protein SDS-PAGE.

2 μg/lane of Recombinant SARS-CoV-2 B.1.617.2 Nucleocapsid His-tag Protein (Catalog # 10999-CV) was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by Coomassie® Blue staining, showing bands at 45-55 kDa under reducing condition.

Formulation, Preparation and Storage

10999-CV
Formulation Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.
Reconstitution Reconstitute at 500 μg/mL in PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Nucleocapsid

SARS-Cov-2, which causes the global pandemic coronavirus disease 2019 (Covid-19), belongs to a family of viruses known as coronaviruses that are commonly comprised of four structural proteins: Spike protein (S), Envelope protein (E), Membrane protein (M), and Nucleocapsid protein (N) (1). While the S, E and M proteins build up the viral envelop, the N protein is involved transcription, replication and packaging of the viral RNA genome into a helical ribonucleocapsid (RNP) (2, 3). The SARS-Cov-2 N protein is a ~45 kDa protein composed of two independent structural domains connected by a linker region. The N-terminal region contains an RNA binding domain, the linker region interacts with the M protein and the C-terminal region contains a self-association domain (2,3). The SARS-Cov-2 N protein shares 91% and 47% amino acid sequence identity with SARS-Cov-1 and MERS N protein, respectively. The SARS-Cov-2 N protein displays VSR (viral suppressor of RNA interference) activity in mammalian cells (4). Several emerging SARS-CoV-2 genomes have been identified with mutations compared to the Wuhan-Hu-1 SARS-CoV-2 reference sequence. First detected in India in December 2020, the B.1.617.2, or Delta, variant is considered a Variant of Concern (VOC) as it contains several mutations that potentially affect viral fitness and transmissibility. As the N protein is an abundant protein during coronavirus infection and displays high immunogenic activity (5, 6), it has been used to develop diagnostic kit for detecting IgM and IgG antibodies against SARS-Cov-2 (7). Within the N protein, there are 4 mutations in the B.1.617.2 variant which might make attractive targets for the development of antiviral therapeutics or potential diagnostic tools.

References

  1. Wu, F. et al. (2020) Nature 579:265.
  2. Chang, C. K. et al. (2006) J. Biomed. Sci. 13:59.
  3. Hurst, K. R. et al. (2009) J. Virol. 83:7221.
  4. Mu, J. et al. (2020) Sci. China Life Sci. doi: 10.1007/s11427-020-1692-1.
  5. Che, X. Y. et al. (2004) J. Clin. Microbiol. 42:2629.
  6. Guan, M. et al. (2004) Clin. Diagn. Lab. Immunol. 11:287.
  7. Liu, W. et al. (2020) J. Clin. Microbiol. doi: 10.1128/JCM.00461-20.

Long Name

Nucleocapsid Protein

Alternate Names

N Protein

Entrez Gene IDs

918763 (HCoV-229E); 3200423 (HCoV-HKU1); 2943504 (HCoV-NL63); 39105221 (HCoV-OC43); 14254601 (MERS-CoV); 1489678 (SARS-CoV); 43740575 (SARS-CoV-2)

Gene Symbol

N

UniProt

Additional Nucleocapsid Products

Product Documents for Recombinant SARS-CoV-2 B.1.617.2 N His-tag Protein, CF

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Note: Certificate of Analysis not available for kit components.

Product Specific Notices for Recombinant SARS-CoV-2 B.1.617.2 N His-tag Protein, CF

For research use only

Reconstitution Calculator

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