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Recombinant S. pyogenes IdeS His-tag Protein, CF

Bio-Techne includes R&D Systems | Catalog # 11341-ID

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11341-ID-050

Key Product Details

Source

E. coli

Accession #

Conjugate

Unconjugated

Applications

Enzyme Activity

Product Specifications

Source

E. coli-derived s. pyogenes IdeS protein
Asp30-Asn339, with an N-terminal Met and C-terminal 6-His tag

Purity

>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

Met

Predicted Molecular Mass

36 kDa

SDS-PAGE

32-35 kDa, under reducing conditions

Activity

Measured by its ability to cleave human IgG.
The DC50 is <25 ng, as measured under the described conditions. The DC50 is defined as the amount of enzyme required to cleave 50% of 1 μg human IgG in 30 minutes at 37 °C. Use of Recombinant S. pyogenes IdeS in the cleavage of other IgGs may require alternative conditions for optimal performance.

Scientific Data Images for Recombinant S. pyogenes IdeS His-tag Protein, CF

Recombinant S. pyogenes IdeS His-tag Protein SDS-PAGE.

2 μg/lane of Recombinant S. pyogenes IdeS His-tag Protein (Catalog # 11341-ID) was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by Coomassie® Blue staining, showing bands at 32-35 kDa.

Formulation, Preparation and Storage

11341-ID
Formulation Supplied as a 0.2 μm filtered solution in Tris and NaCl.
Shipping The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 6 months from date of receipt, -20 to -70 °C as supplied.
  • 3 months, -20 to -70 °C under sterile conditions after opening.

Background: IdeS

IdeS from Streptococcus pyogenes, originally known as Mac-1, is a unique cysteine protease secreted in mature active form with a high degree of specificity for IgG. The structure of IdeS resembles features of the papain cysteine protease family with two distinct domains that interact through a polar interface containing an active site catalytic triad (1). However, IdeS is unique in that it does not contain disulfide bonds or a propeptide and has a RGD motif, known to be important for recognition by integrins (1). Studies have also found IdeS can inhibit neutrophil effector production of reactive oxygen species to repress immune responses in addition to performing IgG hydrolysis (2). IdeS digests all subclasses of human, and some classes of monkey, rabbit and sheep IgG with limited activity on mouse IgG2a and IgG3. IdeS digests IgG at a specific defined site between two glycines in the lower hinger region of the IgG heavy chain generating a homogenous pool of F(ab‘)2 and Fc/2 fragments. The lower hinge region is implicated as being important for Fc receptor recognition and complement binding (2-4). The high specificity and generation of homogeneous products has led to development of analytical strategies utilizing IdeS to characterize monoclonal therapeutic antibodies and related products such as antibody-drug conjugates, Fc-fusion proteins and bispecific antibodies (5). Additionally, animal models have provided significant proof of concept for use of IdeS as a therapeutic in treatment of IgG-mediated autoimmune diseases leading to some success using IdeS in clinical trials (2, 6). 

References

  1. Wenig, K. et al. (2004) PNAS 101:17371. 
  2. von Pawel-Rammingen, U. (2012) J. Innate. Immun. 4:132.
  3. Radaev, S. and P. Sun (2002) Mol. Immunol. 38:1073.
  4. Hulting, G. et al. (2009) FEMS Microbiol. Lett. 298:44.
  5. Sjogren, J. et al. (2016) Analyst 141:3114.
  6. Klontz, E.K. (2022) Transfus. Med. Rev. 36:246.

Long Name

IgG-degrading Enzyme of Streptococcus pyogenes

Entrez Gene IDs

13864345 (S. pyogenes)

Gene Symbol

IDES

UniProt

Additional IdeS Products

Product Documents for Recombinant S. pyogenes IdeS His-tag Protein, CF

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Note: Certificate of Analysis not available for kit components.

Product Specific Notices for Recombinant S. pyogenes IdeS His-tag Protein, CF

For research use only