Recombinant S. pneumoniae beta(1-4)-Galactosidase, CF

R&D Systems, part of Bio-Techne | Catalog # 5549-GH

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5549-GH-100
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Key Product Details

  • R&D Systems E. coli-derived Recombinant S. pneumoniae beta(1-4)-Galactosidase (5549-GH)
  • Quality control testing to verify active proteins with lot specific assays by in-house scientists
  • All R&D Systems proteins are covered with a 100% guarantee

Source

E. coli

Accession #

YP_815516

Conjugate

Unconjugated

Applications

Enzyme Activity

View all beta (1-4)-Galactosidase Proteins and Enzymes »

Product Specifications

Source

E. coli-derived s. pneumoniae beta (1-4)-Galactosidase protein
Thr2-Leu595, with an N-terminal Met and a 6-His tag

Purity

>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

Endotoxin Level

<1.0 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

Met

Predicted Molecular Mass

70 kDa

SDS-PAGE

62 kDa, reducing conditions

Activity

Measured by its ability to cleave a fluorogenic substrate, 4-Methylumbelliferyl-beta -D-galactopyranoside.
The specific activity is >600 pmol/min/μg, as measured under the described conditions.

Formulation, Preparation and Storage

5549-GH
Formulation Supplied as a 0.2 μm filtered solution in Tris and NaCl.
Shipping The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 6 months from date of receipt, -70 °C as supplied.
  • 3 months, -70 °C under sterile conditions after opening.

Background: beta (1-4)-Galactosidase

Majority of secreted and membrane proteins are glycosylated (1, 2). Proper glycosylation could be critical for protein folding and biological functions (3, 4). Galactosides are commonly found on various glycan conjugates and galactosidases are tools for removing these sugar residues (5). beta1-4 Galactosidase from Streptococcus pneumoniae is especially efficient in removing beta1-4 linked non-reducing galactosides from Gal-beta 1-4-GlcNAc or Gal-beta 1-4-GalNAc linkage (6).

References

  1. Lis, H. and Sharon, N. (1993) Eur. J. Biochem. 218:1.
  2. Hart, G.W. (1992) Curr. Opin. Cell Biol., 4:1017.3.
  3. Dwek, R.A. (1995) Biochem. Soc. Trans. 23:1.
  4. Wormald, M.R. and Dwek, R.A. (1999) Structure 7:R155.
  5. Hood, J.M. et al. (1977) Proc. Natl. Acad. Sci. USA 75:113.
  6. Glasgow, L. et al. (1977) J. Biol. Chem. 252:8615.

Alternate Names

beta (14)Galactosidase, beta-Galactosidase-3

UniProt

YP_815516

Additional beta (1-4)-Galactosidase Products

Product Documents for Recombinant S. pneumoniae beta(1-4)-Galactosidase, CF

Product Datasheets

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Product Specific Notices for Recombinant S. pneumoniae beta(1-4)-Galactosidase, CF

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