Recombinant S. marcescens NucA nuclease Protein, CF

R&D Systems, part of Bio-Techne | Catalog # 10038-NAB

R&D Systems, part of Bio-Techne
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10038-NAB-01M
10038-NAB-100
10038-NAB-020
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Key Product Details

Source

E. coli

Accession #

P13717.2

Conjugate

Unconjugated

Applications

Enzyme Activity

View all NucA nuclease Proteins and Enzymes »

Product Specifications

Source

E. coli-derived s. marcescens NucA nuclease protein
Asp22-Asn266

Purity

>97%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

Asp22

Predicted Molecular Mass

27 kDa

SDS-PAGE

27-30 kDa, under reducing conditions

Activity

Measured by its ability to hydrolyze DNA from salmon testes.
The specific activity is >150,000 pmol/min/μg, as measured under the described conditions.

Scientific Data Images for Recombinant S. marcescens NucA nuclease Protein, CF

Recombinant S. marcescens NucA nuclease Protein Enzyme Activity.

Recombinant S. marcescens NucA nuclease Protein, CF (Catalog # 10038-NAB) from R&D Systems and a competitor have similar Specific Activity (pmol/min/µg) measured by its ability to hydrolyze DNA from salmon testes in direct side-by-side comparison using the insert assay protocol described.

Recombinant S. marcescens NucA nuclease Protein SDS-PAGE.

2 μg/lane of Recombinant S. marcescens NucA nuclease Protein (Catalog # 10038-NAB) was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by Coomassie® Blue staining, showing bands at 27-30 kDa, under reducing conditions.

Recombinant S. marcescens NucA nuclease Protein HPLC.

Recombinant S. marcescens NucA nuclease Protein, CF (Catalog # 10038-NAB) purity was determined by HPLC.

Formulation, Preparation and Storage

10038-NAB
Formulation Supplied as a 0.2 μm filtered solution in Tris and NaCl.
Shipping The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 6 months from date of receipt, -20 to -70 °C as supplied.
  • 3 months, -20 to -70 °C under sterile conditions after opening.

Background: NucA nuclease

Serratia marcescens endonuclease NucA is a non-specific nuclease that degrades both single- and double-stranded nucleic acids, including DNA and RNA, but exhibits no proteolytic activity (1) making it ideal for the removal of nucleic acid contaminants from protein samples and in applications where complete digestion of nucleic acids is desirable. NucA is commonly used in bioprocessing applications to reduce viscosity of samples caused by genomic DNA. NucA hydrolyzes internal phosphodiester bonds between nucleotides in nucleic acids to produce 5'-monophosphate oligonucleotides of 3-8 bases in length (2). The active enzyme is a homodimer with two disulfide bonds in each monomer that are crucial to the enzyme activity and stability (3). The optimum pH for enzyme activity is found to be 8.0-9.2. The activity of the recombinant enzyme is measured by a phosphatase coupled assay (4), where the 5'-phosphate of oligosaccharides generated by the enzyme is further released by non-specific alkaline phosphatase and quantitated by Malachite reagents (5).

References

  1. Benedik, MJ and Strych, U. (1998) FEMS Microbiol. Lett. 165:1.
  2. Nestle, M, et al. (1999) J. Biol. Chem. 274:825.
  3. Ball, T.K. et al. (1992) Nucleic Acids Res. 20:4971.
  4. Wu, Z.L. et al. (2011) Glycobiology 21:727.
  5. Van Veldhoven, P.P. and G.P. Mannaerts (1987) Anal. Biochem. 161:45.

Long Name

NucA nuclease

Alternate Names

DcD

Entrez Gene IDs

23387471 (Bacterial)

Gene Symbol

SMDB11_RS05280

UniProt

P13717.2

Additional NucA nuclease Products

Product Documents for Recombinant S. marcescens NucA nuclease Protein, CF

Product Datasheets

Certificate of Analysis

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Product Specific Notices for Recombinant S. marcescens NucA nuclease Protein, CF

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