Recombinant S. marcescens NucA nuclease Protein, CF

Name: Recombinant S. marcescens NucA nuclease Protein, CF
Brand: R&D Systems
SKU: 10038-NA
Rating: 5 (2 reviews)

Catalog # 10038-NA | R&D Systems, Inc. a Bio-Techne Brand

(2)

Product Datasheet / COA / SDS

Compare to Benzonase® Nuclease. Benzonase is a registered trademark of Merck KGaA Corp.

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Key Product Details

Source:	E. coli
Accession #:	P13717
Applications:	Enzyme Activity

View Full Technical Details for Recombinant S. marcescens NucA nuclease Protein, CF

Product Specifications for Recombinant S. marcescens NucA nuclease Protein, CF

Source	E. coli-derived s. marcescens Benzonase® Nuclease/NucA protein Met1-Asn266
Purity	>90%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Predicted Molecular Mass	28.9 kDa
SDS-PAGE	29 kDa, reducing conditions
Activity	Measured by its ability to hydrolyze DNA from salmon testes. The specific activity is >130,000 pmol/min/ug, as measured under the described conditions.

Scientific Data Examples for Recombinant S. marcescens NucA nuclease Protein, CF

	NucA nuclease Digestion of DNA. 2 μg plasmid DNA was incubated with increasing amounts (ng/mL) of RecombinantS. marcescensNucA nuclease (Catalog # 10038-NA) in 50 mM Tris (pH 8.0) and 1 mM MgCl2 for 30 minutes at room temperature. Samples were analyzed by agarose gel electrophoresis and DNA was visualized by ethidium bromide staining.

Formulation, Preparation and Storage

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

10038-NA

Formulation	Supplied as a 0.2 μm filtered solution in Tris and NaCl.
Shipping	The product is shipped with dry ice or equivalent. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage:	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 6 months from date of receipt, -20 to -70 °C as supplied. 3 months, -20 to -70 °C under sterile conditions after opening.

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Background: NucA nuclease

Serratia marcescens endonuclease, also known as Benzonase, is a non-specific nuclease that degrades both single- and double-stranded nucleic acids, including DNA and RNA, but exhibits no proteolytic activity (1); therefore, it is ideal for the removal of nucleic acid contaminants from protein samples and applications where complete digestion of nucleic acids is desirable. NucA (Benzonase) is also commonly used in bioprocessing applications to reduce viscosity of samples caused by genomic DNA. The optimum pH for enzyme activity is found to be 8.0-9.2. It hydrolyzes internal phosphodiester bonds between nucleotides in nucleic acids to produce 5'-monophosphate oligonucleotides of 3-8 bases in length (2). The active enzyme is a homodimer with two disulfide bonds in each monomer that are crucial to the enzyme activity and stability (3). The absolute activity of the recombinant enzyme is measured by a phosphatase coupled assay (4), where the 5'-phosphate of oligosaccharides generated by the enzyme is further released by non-specific alkaline phosphatase and quantitated by Malachite reagents (5).

Item	Value
References	Benedik, MJ and Strych, U. (1998) FEMS Microbiol Lett. 165:1. Nestle, M, et al. (1999) J. Biol. Chem. 274:825. Ball, T.K. et al. (1992) Nucleic Acids Res. 20:4971. Wu, Z.L. et al. (2011) Glycobiology 21:727. Van Veldhoven, P.P. and G.P. Mannaerts (1987) Anal. Biochem. 161:45.
Long Name	NucA nuclease
Entrez Gene IDs	23387471 (Bacterial)
Alternate Names	DcD, NucA nuclease

Product Specific Notices for Recombinant S. marcescens NucA nuclease Protein, CF

Benzonase® is a registered trademark of Merck KGaA Corporation. Bio-Techne Corporation and its affiliates, and their respective products and services, are not sponsored, endorsed, or approved by Merck KGaA.

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Customer Reviews for Recombinant S. marcescens NucA nuclease Protein, CF (2)

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Recombinant S. marcescens NucA Protein, CF

Name: Anonymous

Application: SDS-PAGE Control

Verified Customer | Posted 08/25/2022

1. Ladder2. Sample without NucA3. Sample with NucA
Recombinant S. marcescens NucA Protein, CF

Name: Anonymous

Application: Agarose gel

Verified Customer | Posted 08/25/2022

1. Plasmid control2. Plasmid control in buffer3. Plasmid with nuclease in buffer

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Product Documents for Recombinant S. marcescens NucA nuclease Protein, CF

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