Recombinant Rhesus VSIG8 His-tag Protein, CF

R&D Systems, part of Bio-Techne | Catalog # 11256-VS

R&D Systems, part of Bio-Techne
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11256-VS-050
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Key Product Details

Source

HEK293

Accession #

XP_028702448.1

Conjugate

Unconjugated

Applications

Bioactivity

View all VSIG8 Proteins and Enzymes »

Product Specifications

Source

Human embryonic kidney cell, HEK293-derived rhesus macaque VSIG8 protein
Val24-Gly265, with a C-terminal 6-His tag

Purity

>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

Val24

Predicted Molecular Mass

28 kDa

SDS-PAGE

28-38 kDa, under reducing conditions.

Activity

Measured by its ability to inhibit anti-CD3 antibody induced IL-2 or IFN-gamma secretion by human T cells. The ED50 for this effect 0.250-5.00 μg/mL.

Scientific Data Images for Recombinant Rhesus VSIG8 His-tag Protein, CF

Recombinant Rhesus VSIG8 His-tag Protein Bioactivity.

Measured by its ability to inhibit anti-CD3 antibody induced IL-2 or IFN-gamma secretion by human T cells. The ED50 for this effect 0.250-5.00 μg/mL.

Recombinant Rhesus VSIG8 His-tag Protein SDS-PAGE.

2 μg/lane of Recombinant Rhesus VSIG8 His-tag Protein (Catalog # 11256-VS) was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by Coomassie® Blue staining, showing bands at 28-38 kDa.

Formulation, Preparation and Storage

11256-VS
Formulation Lyophilized from a 0.2 μm filtered solution in Citric Acid and NaCl with Trehalose.
Reconstitution Reconstitute at 500 μg/mL in PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: VSIG8

VSIG8 (V-set and immunoglobulin domain containing 8), also known as C1orf204, is an approximately 45 kDa type I transmembrane protein of the B7 family within the Ig superfamily. Based on its similarity with human VSIG8, rhesus VSIG8 is predicted to consist of a 242 amino acid (aa) extracellular domain (ECD) containing two V-type Ig-like domains, a 21 aa transmembrane domain, and a 130 aa cytoplasmic domain. Within the ECD, rhesus VSIG8 shares 99% and 100% aa identity with human and cynomolgus VSIG8, respectively.  VSIG8 was identified from proteomic analysis of human hair shafts (1, 2). It is expressed in the hair follicle and shaft, superficial layers of the nail matrix, and superficial layers of oral epithelium (3). R&D Systems in-house testing indicates that VSIG8 inhibits the production of IL-2 by activated T cells.

References

  1. Rice, R.H. et al. (2010) J. Proteome Res. 9:6752.
  2. Lee, Y.J. et al. (2006) Mol. Cell. Proteomics 5:789.
  3. Rice, R.H. et al. (2011) J. Invest. Dermatol. 131:1936.

Long Name

V-Set and Immunoglobulin Domain Containing 8

Alternate Names

V-set and immunoglobulin domain containing 8

Entrez Gene IDs

391123 (Human); 240916 (Mouse); 289236 (Rat); 102123183 (Cynomolgus Monkey)

Gene Symbol

VSIG8

UniProt

XP_028702448.1

Additional VSIG8 Products

Product Documents for Recombinant Rhesus VSIG8 His-tag Protein, CF

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Certificate of Analysis

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Product Specific Notices for Recombinant Rhesus VSIG8 His-tag Protein, CF

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