Recombinant Rhesus Macaque IFN-gamma Protein

Name: Recombinant Rhesus Macaque IFN-gamma Protein
Brand: R&D Systems
SKU: 961-RM

Catalog # 961-RM | R&D Systems, Inc. a Bio-Techne Brand

Citations (1)

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Key Product Details

Source:	E. coli
Accession #:	P63310
Applications:	Bioactivity

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Technical Data
Citations (1) / Reviews

Product Specifications for Recombinant Rhesus Macaque IFN-gamma Protein

Source	E. coli-derived rhesus macaque IFN-gamma protein Gln24-Gln165, with an N-terminal Met
Purity	>97%, by SDS-PAGE under reducing conditions and visualized by silver stain.
Endotoxin level	<0.10 EU per 1 μg of the protein by the LAL method.
N-terminal sequence Analysis	Met
Predicted Molecular Mass	16.9 kDa
SDS-PAGE	16.9 kDa
Activity	Measured in anti-viral assays using HeLa human cervical epithelial carcinoma cells infected with encephalomyocarditis (EMC) virus. Meager, A. (1987) in Lymphokines and Interferons, a Practical Approach. Clemens, M.J. et al. (eds): IRL Press. 129. The ED₅₀ for this effect is 50.0-500 ng/mL.

Formulation, Preparation and Storage

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

961-RM

Carrier

Formulation	Lyophilized from a 0.2 μm filtered solution in Tris with BSA as a carrier protein.
Reconstitution	Reconstitute at 25 μg/mL in sterile PBS containing at least 0.1% human or bovine serum albumin.
Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage:	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 3 months, -20 to -70 °C under sterile conditions after reconstitution.

961-RM/CF

Carrier Free

Formulation	Lyophilized from a 0.2 μm filtered solution in Tris.
Reconstitution	Reconstitute at 100 μg/mL in sterile PBS.
Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage:	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: IFN-gamma

Interferon-gamma (IFN-gamma ), also known as type II or immune interferon, exerts a wide range of immunoregulatory activities and is considered to be the prototype proinflammatory cytokine (1, 2). Mature rhesus IFN-gamma exists as a noncovalently linked homodimer of 20 - 25 kDa variably glycosylated subunits (3). It shares 90% amino acid sequence identity with human IFN-gamma, 57% - 66% with bovine, canine, equine, feline, and porcine IFN-gamma, and 37% - 44% with cotton rat, mouse, and rat IFN‑ gamma. IFN‑ gamma dimers bind to IFN-gamma RI (alpha subunits) which then interact with IFN-gamma RII (beta subunits) to form the functional receptor complex of two alpha and two beta subunits. Inclusion of IFN-gamma RII increases the binding affinity for ligand and the efficiency of signal transduction (4, 5). IFN‑ gamma is produced by a variety of immune cells under inflammatory conditions, notably by T cells and NK cells (6). It plays a key role in host defense by promoting the development and activation of Th1 cells, chemoattraction and activation of monocytes and macrophages, upregulation of antigen presentation molecules, and immunoglobulin class switching in B cells. It also exhibits antiviral, antiproliferative, and apoptotic effects (6, 7). In addition, IFN‑ gamma functions as an anti-inflammatory mediator by promoting the development of regulatory T cells and inhibiting Th17 cell differentiation (8, 9). The pleiotropic effects of IFN‑ gamma contribute to the development of multiple aspects of atherosclerosis (7).

Item	Value
References	Billiau, A. and P. Matthys (2009) Cytokine Growth Factor Rev. 20:97. Pestka, S. et al. (2004) Immunol. Rev. 202:8. Villinger, F. et al. (1995) J. Immunol. 155:3946. Marsters, S.A. et al. (1995) Proc. Natl. Acad. Sci. 92:5401. Krause, C.D. et al. (2000) J. Biol. Chem. 275:22995. Schroder, K. et al. (2004) J. Leukoc. Biol. 75:163. McLaren, J.E. and D.P. Ramji (2009) Cytokine Growth Factor Rev. 20:125. Muhl, H. and J. Pfeilschifter (2003) Int. Immunopharmacol. 3:1247. Kelchtermans, H. et al. (2008) Trends Immunol. 29:479.
Long Name	Interferon gamma
Entrez Gene IDs	3458 (Human); 15978 (Mouse); 25712 (Rat); 396991 (Porcine); 281237 (Bovine); 403801 (Canine); 493965 (Feline)
Alternate Names	IFG, IFI, IFN-gamma, IFNG, IFNgamma, Immune interferon, interferon gamma, interferon, gamma

Citations for Recombinant Rhesus Macaque IFN-gamma Protein (1)

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Citations are publications that use Bio-Techne products. Selected citations for Recombinant Rhesus Macaque IFN-gamma Protein include:

Species: Primate - Macaca mulatta (Rhesus Macaque)
Sample Types: Whole Cells
Applications: Bioassay

Clay CC et al. (2007-08-22), Neuroinvasion of fluorescein-positive monocytes in acute simian immunodeficiency virus infection. J. Virol., 2007-081(21):12040-8.
PMID: 17715237

Recombinant Rhesus Macaque IFN-gamma Protein

Catalog # 961-RM | R&D Systems, Inc. a Bio-Techne Brand

Key Product Details

Product Specifications for Recombinant Rhesus Macaque IFN-gamma Protein

Formulation, Preparation and Storage

Carrier Free

961-RM

Carrier

961-RM/CF

Carrier Free

Reconstitution Calculator

Background: IFN-gamma

Citations for Recombinant Rhesus Macaque IFN-gamma Protein (1)

Product Documents for Recombinant Rhesus Macaque IFN-gamma Protein

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