Recombinant R.gnavus alpha-L-Fucosidase His-tag Protein, CF

R&D Systems, part of Bio-Techne | Catalog # 11385-GH

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11385-GH-020
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Key Product Details

  • R&D Systems E. coli-derived Recombinant R.gnavus alpha-L-Fucosidase His-tag Protein (11385-GH)
  • Quality control testing to verify active proteins with lot specific assays by in-house scientists
  • All R&D Systems proteins are covered with a 100% guarantee

Source

E. coli

Accession #

WP_243035407.1

Conjugate

Unconjugated

Applications

Enzyme Activity

View all alpha-L-Fucosidase Proteins and Enzymes »

Product Specifications

Source

E. coli-derived alpha-L-Fucosidase protein
Glu35-Gly583 with an N-terminal Met and 6-His tag

Purity

>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

Met

Predicted Molecular Mass

62 kDa

SDS-PAGE

62-68 kDa, under reducing conditions

Activity

Measured by its ability to cleave alpha3 Fucose.
Recombinant a-L-Fucosidase will cleave >50% Cy5-Fuc labeled Lewis X (Catalog # GL306), as measured under the described conditions.

Scientific Data Images for Recombinant R.gnavus alpha-L-Fucosidase His-tag Protein, CF

Recombinant R. gnavus alpha‑L‑Fucosidase His-tag Protein Enzyme Activity Diagram.

Recombinant R. gnavus alpha-L-Fucosidase His-tag Protein, CF (Catalog # 11385-GH) has specificity for both Lewis A ( alpha4) and Lewis X ( alpha3) link fucose. Sialyation of Lewis A or Lewis X does not affect the substrate recognition of alpha-L-fucosidase.

Recombinant R. gnavus alpha‑L‑Fucosidase His-tag Protein SDS-PAGE.

2 μg/lane of Recombinant R. gnavus alpha‑L‑Fucosidase His-tag Protein (Catalog # 11385-GH) was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by Coomassie® Blue staining, showing bands at 62-68 kDa under reducing conditions.

Recombinant R. gnavus alpha‑L‑Fucosidase His-tag Protein Enzyme Activity.

Lane 1 contained fluorescent substrate glycan Cy5-Fuc labeled Lewis X. Following treatment with Recombinant R. gnavus alpha-L-Fucosidase His-tag Protein, CF (Catalog # 11385-GH) both alpha3 linked Fucose on GlcNAc were removed and a mobility shift was observed. SDS-PAGE gel was imaged using the red fluorescent channel.

Formulation, Preparation and Storage

11385-GH
Formulation Supplied as a 0.2 μm filtered solution in Tris and NaCl.
Shipping The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 6 months from date of receipt, -20 to -70 °C as supplied.
  • 3 months, -20 to -70 °C under sterile conditions after opening.

Background: alpha-L-Fucosidase

Recombinant R. gnavus alpha-L-Fucosidase His-tag ( alpha-L-Fuc) catalyzes the hydrolysis of terminal alpha-L-Fucose. alpha-L-Fuc is monomeric and consists of two distinct domains, an N-terminal catalytic domain comprising residues 46-366 and a C-terminal F5/8 Type C domain covering residues 385-526 (1). Residues 23-45 wrap around the C-terminal beta-sandwich domain (1). R. gnavus is an early colonizer of the human gut but persists in healthy adults (2-3). An increasing number of studies are reporting a disproportionate representation of R. gnavus in diseases, such as inflammatory bowel disease (4). To access a source of nutrients, gut bacteria encode alpha-L-fucosidases that catalyze the hydrolysis of terminal alpha-L-fucosidic linkages. alpha-L-Fuc has the capacity to recognize fucosylated glycans and to hydrolyze both alpha1-3 (Lewis X) and alpha1–4 (Lewis A) fucosyl linkages although the preferred substrate is sialyated Lewis X epitope (1). This fucosidase specificity can potentially be exploited for use in human disease diagnostic assays, as a tool to identify N-glycan biomarkers of disease, and for glycoprofiling biopharmaceutical glycoproteins. The activity of alpha-L-Fuc is demonstrated in an electrophoretic gel mobility shift assay using a fluorophore-labeled glycan Cy5-Fuc labeled Lewis X as the substrate (5).

References

  1. Wu, H. et al. (2021) Cell. Mol. Life Sci. 78:675.
  2. Sagheddu, V. et al. (2016) Front. Pediatr. 4:57.
  3. Qin, J. et al. (2010) Nature 464:59.
  4. Hall, A.B. et al. (2017) Genome Med. 9:103.
  5. Wu, Z.L. et al. (2020) Glycobiology 30:970.

Alternate Names

alphaLFucosidase

Entrez Gene IDs

897238 (T. maritima)

Gene Symbol

TM_RS01570

UniProt

WP_243035407.1

Additional alpha-L-Fucosidase Products

Product Documents for Recombinant R.gnavus alpha-L-Fucosidase His-tag Protein, CF

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Product Specific Notices for Recombinant R.gnavus alpha-L-Fucosidase His-tag Protein, CF

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