Recombinant Rat VEGF 164 Protein

Name: Recombinant Rat VEGF 164 Protein
Brand: R&D Systems
SKU: 564-RV
Rating: 5 (1 reviews)

R&D Systems, Inc. a Bio-Techne Brand

Catalog # 564-RV

Citations (33)

(1)

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Key Product Details

Source:	NS0
Accession #:	AAL07526.1
Structure / Form:	Disulfide-linked homodimer
Applications:	Bioactivity

View Full Technical Details for Recombinant Rat VEGF 164 Protein

Product Specifications for Recombinant Rat VEGF 164 Protein

Source	Mouse myeloma cell line, NS0-derived rat VEGF protein Ala27-Arg190
Purity	>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Endotoxin level	<0.10 EU per 1 μg of the protein by the LAL method.
N-terminal sequence Analysis	Ala27
Predicted Molecular Mass	19.2 kDa (monomer)
SDS-PAGE	25 kDa, reducing conditions
Activity	Measured in a cell proliferation assay using HUVEC human umbilical vein endothelial cells. Conn, G. et al. (1990) Proc. Natl. Acad. Sci. USA 87:1323. The ED₅₀ for this effect is 0.75‑3.75 ng/mL.

Scientific Data Examples for Recombinant Rat VEGF 164 Protein

	Recombinant Rat VEGF 164 Protein Bioactivity Recombinant Rat VEGF₁₆₄(Catalog # 564‑RV) stimulates cell proliferation in HUVEC human umbilical vein endothelial cells in a dose-dependent manner. The ED₅₀ is 0.75-3.75 ng/mL.
	Recombinant Rat VEGF 164 Protein SDS-PAGE 1 μg/lane of Recombinant Rat VEGF₁₆₄was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by silver staining, showing bands at 25 kDa and 54 kDa, respectively.

Formulation, Preparation and Storage

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

564-RV

Carrier

Formulation	Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.
Reconstitution	Reconstitute at 100 μg/mL in sterile PBS containing at least 0.1% human or bovine serum albumin.
Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage:	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 3 months, -20 to -70 °C under sterile conditions after reconstitution.

564-RV/CF

Carrier Free

Formulation	Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution	Reconstitute at 100 μg/mL in sterile PBS.
Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage:	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: VEGF

Vascular endothelial growth factor (VEGF or VEGF-A), also known as vascular permeability factor (VPF), is a potent mediator of both angiogenesis and vasculogenesis in the fetus and adult (1-3). It is a member of the PDGF family that is characterized by a cysteine-knot structure formed by eight conserved cysteine residues (4). Alternately spliced isoforms of 121, 145, 165, 183, 189, and 206 amino acids (aa) have been identified in humans, with 120, 164 and 188 aa isoforms found in rat and mouse (2, 4). Isoforms other than VEGF₁₂₀ and VEGF₁₂₁ contain basic heparin-binding regions and are not freely diffusible (4). Rat VEGF₁₆₄ shares 97% aa sequence identity with corresponding regions of mouse, 88% with human and bovine, 89% with porcine and canine, and 90% with feline and equine VEGF, respectively. VEGF binds the type I transmembrane receptor tyrosine kinases VEGF R1 (also called Flt-1) and VEGF R2 (Flk-1/KDR) on endothelial cells (4). Although affinity is highest for binding to VEGF R1, VEGF R2 appears to be the primary mediator of VEGF angiogenic activity (3, 4). Human VEGF₁₆₅ binds the semaphorin receptor, neuropilin-1 and promotes complex formation with VEGF R2 (5). VEGF is required during embryogenesis to regulate the proliferation, migration, and survival of endothelial cells (3, 4). In adults, VEGF functions mainly in wound healing and the female reproductive cycle (3). Pathologically, it is involved in tumor angiogenesis and vascular leakage (6, 7). Circulating VEGF levels correlate with disease activity in autoimmune diseases such as rheumatoid arthritis, multiple sclerosis and systemic lupus erythematosus (8). VEGF is induced by hypoxia and cytokines such as IL-1, IL-6, IL-8, oncostatin M and TNF-alpha (3, 4, 9).

Item	Value
References	Conn, G. et al. (1990) J. Biol. Chem. 87:2628. Ishii, H. et al. (2001) Arch. Oral Biol. 46:77. Byrne, A.M. et al. (2005) J. Cell. Mol. Med. 9:777. Robinson, C.J. and Stringer, S.E. (2001) J. Cell. Sci. 114:853. Pan, Q. et al. (2007) J. Biol. Chem. 282:24049. Weis, S.M. & D.A. Cheresh (2005) Nature 437:497. Thurston, G. (2002) J. Anat. 200:575. Carvalho, J.F. et al. (2007) J. Clin. Immunol. 27:246. Angelo, L.S. & R. Kurzrock (2007) Clin. Cancer Res. 13:2825.
Long Name	Vascular Endothelial Growth Factor
Entrez Gene IDs	7422 (Human); 22339 (Mouse); 83785 (Rat); 281572 (Bovine); 403802 (Canine); 493845 (Feline); 30682 (Zebrafish)
Alternate Names	MVCD1, VAS, VEGF, VEGF-A, VEGFA, VEGFMGC70609, VPF, VPFvascular endothelial growth factor, Vascular permeability factor, Vasculotropin, vascular endothelial growth factor A

Citations for Recombinant Rat VEGF 164 Protein (33)

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Citations are publications that use Bio-Techne products. Selected citations for Recombinant Rat VEGF 164 Protein include:

Species: Rat
Sample Types: In Vivo
Applications: In Vivo

L Kalkowski et al. (2022), Local autoimmune encephalomyelitis model in a rat brain with precise control over lesion placement PLoS ONE, 17(1):e0262677.
PMID: 35061807
Species: Rat
Sample Types: Whole Cells
Applications: Bioassay

P Wang et al. (2022), beta-Catenin promotes long-term survival and angiogenesis of peripheral blood mesenchymal stem cells via the Oct4 signaling pathway Experimental Molecular Medicine, 2022;0(0).
PMID: 36050404
Species: Rat
Sample Types: Nanofibrous Membrane
Applications: Bioassay

DP Wang et al. (2021), Neuroprotective Effects of VEGF-A Nanofiber Membrane and FAAH Inhibitor URB597 Against Oxygen-Glucose Deprivation-Induced Ischemic Neuronal Injury International Journal of Nanomedicine, 16(0):3661-3678.
PMID: 34093011
Species: Rat
Sample Types: Whole Cell
Applications: Bioassay

N Froger et al. (2020), VEGF is an autocrine/paracrine neuroprotective factor for injured retinal ganglion neurons Sci Rep, 10(1):12409.
PMID: 32710087
Species: Rat
Sample Types: In Vivo
Applications: In Vivo

M Díaz-Corán et al. (2020), Norrin restores blood-retinal barrier properties after vascular endothelial growth factor-induced permeability J. Biol. Chem., 0(0).
PMID: 32086377
Species: Rat
Sample Types: Whole Cells
Applications: Bioassay

SA Mousavi et al. (2019), Receptor-Mediated Endocytosis of VEGF-A in Rat Liver Sinusoidal Endothelial Cells Biomed Res Int, 2019(0):5496197.
PMID: 31583245
Species: Rat
Sample Types: Whole Cells
Applications: Bioassay

J Chen et al. (2019), The combinatory effect of sinusoidal electromagnetic field and VEGF promotes osteogenesis and angiogenesis of mesenchymal stem cell-laden PCL/HA implants in a rat subcritical cranial defect Stem Cell Res Ther, 10(1):379.
PMID: 31842985
Species: Rat
Sample Types: Whole Tissue
Applications: Bioassay

J Li et al. (2019), Dietary compound glycyrrhetinic acid suppresses tumor angiogenesis and growth by modulating antiangiogenic and proapoptotic pathways in vitro and in vivo J. Nutr. Biochem., 77(0):108268.
PMID: 31830590
Species: Rat
Sample Types: In Vivo
Applications: Bioassay

R Lopez-Bell et al. (2019), Growth factor signaling regulates mechanical nociception in flies and vertebrates J. Neurosci., 0(0).
PMID: 31138657
Species: Human
Sample Types: Recombinant Protein
Applications: Bioassay

GA Rodrigues et al. (2018), Functional Characterization of Abicipar-Pegol, an Anti-VEGF DARPin Therapeutic That Potently Inhibits Angiogenesis and Vascular Permeability Invest. Ophthalmol. Vis. Sci., 59(15):5836-5846.
PMID: 30535424
Species: Rat
Sample Types: Whole Cells
Applications: Bioassay

C Chang et al. (2018), Downregulation of TRPC6 expression is a critical molecular event during FK506 treatment for overactive bladder Cell Calcium, 77(0):8-19.
PMID: 30476735
Species: Rat
Sample Types: In Vivo
Applications: In Vivo

M Li et al. (2018), Effects of Triple-Mutated Hypoxia-Inducible Factor-1? on Angiogenesis and Cardiac Function Improvement in Rats with Myocardial Infarction Cell. Physiol. Biochem., 50(6):2329-2340.
PMID: 30423574
Species: Rat
Sample Types: In Vivo
Applications: In Vivo

(2016), Angiogenic microspheres promote neural regeneration and motor function recovery after spinal cord injury in rats Sci Rep, 6(0):33428.
PMID: 27641997
Species: Rat
Sample Types: Whole Cells
Applications: Bioassay

M Vitacolonn (2016), In-vivo quantification of the revascularization of a human acellular dermis seeded with EPCs and MSCs in co-culture with fibroblasts and pericytes in the dorsal chamber model in pre-irradiated tissue Cell Tissue Bank, 0(0).
PMID: 28004288
Species: Rat
Sample Types: Whole Tissue
Applications: Bioassay

(2016), Caspase-3 dependent nitrergic neuronal apoptosis following cavernous nerve injury is mediated via RhoA and ROCK activation in major pelvic ganglion Sci Rep, 6(0):29416.
PMID: 27388816
Species: Rat
Sample Types: Complex Sample Type
Applications: Bioassay

Sjoqvist S et al. (2014), Experimental orthotopic transplantation of a tissue-engineered oesophagus in rats. Nat Commun, 5(0):3562.
PMID: 24736316
Species: Rat
Sample Types: In Vivo
Applications: In Vivo

Weinkopff T et al. (2014), Filarial excretory-secretory products induce human monocytes to produce lymphangiogenic mediators. PLoS Negl Trop Dis, 8(7):e2893.
PMID: 25010672
Species: Rat
Sample Types: In Vivo
Applications: In Vivo

Trichonas G et al. (2010), A novel nonradioactive method to evaluate vascular barrier breakdown and leakage. Invest. Ophthalmol. Vis. Sci., 51(3):1677-82.
PMID: 19875655
Species: Human
Sample Types: Cell Culture Supernates
Applications: Binding Assay

Irani Y et al. (2009), Production of scFv antibody fragments from a hybridoma with functional activity against human vascular endothelial growth factor. Hybridoma (Larchmt), 28(3):205-9.
PMID: 19445630
Species: Mouse
Sample Types: Whole Cells
Applications: Bioassay

Hu J et al. (2009), Blockade of Wnt signaling inhibits angiogenesis and tumor growth in hepatocellular carcinoma. Cancer Res., 69(17):6951-9.
PMID: 19690140
Species: Rat
Sample Types: In Vivo
Applications: In Vivo

Kempen DH et al. (2009), Effect of local sequential VEGF and BMP-2 delivery on ectopic and orthotopic bone regeneration. Biomaterials, 30(14):2816-25.
PMID: 19232714
Species: Rat
Sample Types: In Vivo
Applications: In Vivo

Rocha FG et al. (2008), The effect of sustained delivery of vascular endothelial growth factor on angiogenesis in tissue-engineered intestine. Biomaterials, 29(19):2884-90.
PMID: 18396329
Species: Rat
Sample Types: In Vivo
Applications: In Vivo

Skondra D et al. (2008), Characterization of azurocidin as a permeability factor in the retina: involvement in VEGF-induced and early diabetic blood-retinal barrier breakdown. Invest. Ophthalmol. Vis. Sci., 49(2):726-31.
PMID: 18235021
Species: Rat
Sample Types: Whole Cells
Applications: Bioassay

Marko SB et al. (2008), VEGF promotes vascular sympathetic innervation. Am. J. Physiol. Heart Circ. Physiol., 294(6):H2646-52.
PMID: 18408130
Species: Rat
Sample Types: Whole Cells
Applications: Bioassay

Cho CH et al. (2007), Homogeneous differentiation of hepatocyte-like cells from embryonic stem cells: applications for the treatment of liver failure. FASEB J., 22(3):898-909.
PMID: 17942827
Species: Rat
Sample Types: In Vivo
Applications: In Vivo

Kuiper EJ et al. (2007), Effect of VEGF-A on expression of profibrotic growth factor and extracellular matrix genes in the retina. Invest. Ophthalmol. Vis. Sci., 48(9):4267-76.
PMID: 17724216
Species: Rat
Sample Types: In Vivo
Applications: In Vivo

Namisaki T et al. (2006), Salvage effect of the vascular endothelial growth factor on chemically induced acute severe liver injury in rats. J. Hepatol., 44(3):568-75.
PMID: 16310882
Species: Rat
Sample Types: In Vivo
Applications: In Vivo

Rhodes KE et al. (2006), The injury response of oligodendrocyte precursor cells is induced by platelets, macrophages and inflammation-associated cytokines. Neuroscience, 140(1):87-100.
PMID: 16631314
Species: Rat
Sample Types: In Vivo
Applications: In Vivo

Lennernas B et al. (2004), Antiangiogenic effect of metronomic paclitaxel treatment in prostate cancer and non-tumor tissue in the same animals: a quantitative study. APMIS, 112(3):201-9.
PMID: 15153162
Species: N/A
Sample Types: N/A
Applications: ELISA (Standard)

Rota R et al. (2004), Marked inhibition of retinal neovascularization in rats following soluble-flt-1 gene transfer. J Gene Med, 6(9):992-1002.
PMID: 15352072
Species: Xenopus
Sample Types: In Vivo
Applications: In Vivo

Mogi K et al. (2003), Xenopus neurula left-right asymmetry is respeficied by microinjecting TGF-beta5 protein. Int. J. Dev. Biol., 47(1):15-29.
PMID: 12653248
Species: Rat
Sample Types: Whole Cells
Applications: Bioassay

Maynard SE et al. (2003), Excess placental soluble fms-like tyrosine kinase 1 (sFlt1) may contribute to endothelial dysfunction, hypertension, and proteinuria in preeclampsia. J. Clin. Invest., 111(5):649-58.
PMID: 12618519
Species: Rat
Sample Types: In Vivo
Applications: In Vivo

Lopez-Bellido R et al. (0), Growth Factor Signaling Regulates Mechanical Nociception in Flies and Vertebrates. J Neurosc39(30):6012-6030.
PMID: 31138657

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Recombinant Rat VEGF 164 Protein

Name: Leslie Priddy

Application: In vivo study

Verified Customer | Posted 04/01/2018

Recombinant Rat VEGF 164 Protein

R&D Systems, Inc. a Bio-Techne Brand

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Product Specifications for Recombinant Rat VEGF 164 Protein

Scientific Data Examples for Recombinant Rat VEGF 164 Protein

Formulation, Preparation and Storage

Carrier Free

564-RV

Carrier

564-RV/CF

Carrier Free

Reconstitution Calculator

Background: VEGF

Citations for Recombinant Rat VEGF 164 Protein (33)

Customer Reviews for Recombinant Rat VEGF 164 Protein (1)

Recombinant Rat VEGF 164 Protein

Product Documents for Recombinant Rat VEGF 164 Protein

Product Datasheets

COA

Certificate of Analysis

SDS

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