Recombinant Rat TrkA Fc Chimera Protein, CF

Name: Recombinant Rat TrkA Fc Chimera Protein, CF
Brand: R&D Systems
SKU: 1056-TK

R&D Systems, Inc. a Bio-Techne Brand

Catalog # 1056-TK

Citations (3)

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Key Product Details

Source:	NS0
Accession #:	P35739
Structure / Form:	Disulfide-linked homodimer
Applications:	Bioactivity

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Product Specifications for Recombinant Rat TrkA Fc Chimera Protein, CF

Source

Mouse myeloma cell line, NS0-derived rat TrkA protein

Rat TrkA (Ala33 - Pro418) & (Ala34 - Pro418) Accession # P35739	IEGRMD	Human IgG₁ (Pro100 - Lys330)
N-terminus		C-terminus

Purity

>90%, by SDS-PAGE under reducing conditions and visualized by silver stain

Endotoxin level

<0.10 EU per 1 μg of the protein by the LAL method.

N-terminal sequence Analysis

Ala33 & Ala34

Predicted Molecular Mass

69 kDa (monomer)

SDS-PAGE

120-130 kDa, reducing conditions

Activity

Measured by its ability to inhibit NGF-induced proliferation of TF‑1 human erythroleukemic cells. The ED₅₀ for this effect is 10-40 ng/mL in the presence of 10 ng/mL of rrNGF.

Formulation, Preparation and Storage

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

1056-TK

Formulation	Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution	Reconstitute at 100 μg/mL in sterile PBS. Reconstitution Buffer Available: Size / Price Qty Loading... RB01:
Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage:	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 3 months, -20 to -70 °C under sterile conditions after reconstitution.

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Background: TrkA

Trk A, the product of the proto-oncogene trk, is a member of the neurotrophic tyrosine kinase receptor family that has three members. Trk A, Trk B and Trk C preferentially bind NGF, NT-4 and BDNF, and NT-3, respectively. All Trk family proteins share a conserved complex subdomain organization consisting of a signal peptide, two cysteine-rich domains, a cluster of three leucine-rich motifs, and two immunoglobulin-like domains in the extracellular region, as well as an intracellular region that contains the tyrosine kinase domain. Two distinct rat Trk A isoforms (TrkA-I and Trk-A-II) that differ by a 6-amino acid insertion in their extracellular domain have been identified. The longer Trk A isoform is the only isoform expressed within neuronal tissues whereas the shorter Trk A-I is expressed mainly in non-neuronal tissues. NGF binds to Trk A with low affinity and activates its cytoplasmic kinase, initiating a signaling cascade that mediates neuronal survival and differentiation. Higher affinity binding of NGF requires the coexpression of Trk A with the p75 NGF receptor (NGF R), a member of the tumor necrosis factor receptor superfamily. NGF R binds all neurotrophins with low affinity and modulates Trk activity as well as alters the specificity of Trk receptors for their ligands. NGF R can also mediate cell death when expressed independent of Trk.

Item	Value
References	Esposito, D. et al. (2001) J. Biol. Chem. 276:32687. Sofroniew, M.V. et al. (2001) Annu. Rev. Neurosci. 24:1217.
Long Name	Neurotrophic Tyrosine Kinase Receptor A
Entrez Gene IDs	4914 (Human); 18211 (Mouse); 59109 (Rat)
Alternate Names	DKFZp781I14186, EC 2.7.10, EC 2.7.10.1, MTChigh affinity nerve growth factor receptor, NTRK-1, NTRK1, Neurotrophic tyrosine kinase receptor type 1, TRK1-transforming tyrosine kinase protein, TRKAOncogene TRK, TRKTRK1, Trk-A, TrkA, neurotrophic tyrosine kinase, receptor, type 1, p140-TrkA, tyrosine kinase receptor A

Citations for Recombinant Rat TrkA Fc Chimera Protein, CF (3)

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Citations are publications that use Bio-Techne products. Selected citations for Recombinant Rat TrkA Fc Chimera Protein, CF include:

Species: Rat
Sample Types: Whole Cells
Applications: Cell Culture

HS Domingues et al. (2020), Pushing myelination: developmental regulation of myosin expression drives oligodendrocyte morphological differentiation J. Cell. Sci., 0(0).
PMID: 32620697
Species: Mouse
Sample Types: Whole Cells
Applications: Bioassay

(2012), Neurotrophic actions initiated by proNGF in adult sensory neurons may require peri-somatic glia to drive local cleavage to NGF. J. Neurochem., 122(3):523-36.
PMID: 22621370
Species: Mouse
Sample Types: Whole Cells
Applications: Bioassay

Kim YE et al. (2010), Monitoring apoptosis and neuronal degeneration by real-time detection of phosphatidylserine externalization using a polarity-sensitive indicator of viability and apoptosis. Nat Protoc, 5(8):1396-405.
PMID: 20671723

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