Product Specifications for Recombinant Rat Reg3G Protein, CF
E. coli-derived rat Reg3G protein Glu27-Ala174, with an N-terminal Met and a C-terminal 6-His tag
>95%, by SDS-PAGE with silver staining
<0.10 EU per 1 μg of the protein by the LAL method.
N-terminal sequence Analysis
Predicted Molecular Mass
17 kDa, reducing conditions
Measured in a cell proliferation assay using RT4‑D6P2T rat schwannoma cells. The ED50 for this effect is 0.15-0.75 μg/mL.
Formulation, Preparation and Storage
What does CF mean?
CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our
Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant
protein to be stored at a more dilute concentration.
The carrier free version does not contain BSA.
What formulation is right for me?
In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or
as an ELISA standard.
In contrast, the carrier free protein is recommended for applications, in which the presence of BSA
Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitute at 500 μg/mL in sterile PBS.
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage:
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
3 months, -20 to -70 °C under sterile conditions after reconstitution.
Regenerating islet-derived protein 3-gamma (Reg3G), also known as pancreatitis-associated protein 3 (PAP3), is one of several secreted Reg proteins that contain a single C-type lectin domain and contribute to anti-microbial immunity in the gut (1, 2). Reg3G is secreted as a 148 amino acid (aa) proprotein, and 11 residues are cleaved from the N-terminus to generate the 15 kDa mature protein (3). This short propeptide serves as an inhibitor that limits the activity of the proprotein (3, 4). Mature rat Reg3G shares 65% and 85% aa sequence identity with human and mouse Reg3G, respectively. Reg3G is expressed by Paneth cells of the small intestine and is released into the gut lumen (5). It is up-regulated in gastrointestinal epithelium by bacterial inflammation or by defects in the mucous lining (5-8). Its expression and secretion is also induced in the lung in response to MRSA (drug resistant S. aureus) and in damaged skeletal muscle and peripheral neurons (4, 9). Reg3G binds to bacterial peptidoglycans, exerts bacteriostatic and bactericidal activities, and protects the mucosa against bacterial invasion (4, 5, 10, 11). It also inhibits inflammation by limiting the number of Th1 cells in the lamina propria (11). R&D in-house data indicate that Reg3G can induce the proliferation of Schwann cells, a property shared with Reg3B (12).
Parikh, A. et al. (2012) Biomol. Concepts 3:57.
Narushima, Y. et al. (1997) Gene 185:159.
Mukherjee, S. et al. (2009) J. Biol. Chem. 284:4881.
Choi, S-M. et al. (2013) J. Exp. Med. 210:551.
Cash, H.L. et al. (2006) Science 313:1126.
Lee, K.S. et al. (2012) PLoS ONE 7:e30786.
Keilbaugh, S.A. et al. (2005) Gut 54:623.
Burger-van Passen, N. et al. (2012) PLoS ONE 7:e38798.
Klasan, G.S. et al. (2014) Muscle Nerve 49:61.
Brandl, K. et al. (2007) J. Exp. Med. 204:1891.
Vaishnava, S. et al. (2011) Science 334:255.
Livesey, F.J. et al. (1997) Nature 390:614.
Regenerating Islet-derived III gamma
Entrez Gene IDs
130120 (Human); 19695 (Mouse); 24620 (Rat)
LPPM429, MGC118999, MGC119001, PAP IB, PAP-1B, PAP1B, PAP1BMGC118998, PAP3, PAPIB, PAPIII, Pancreatitis-associated protein 1B, Pancreatitis-associated protein IB, REG-3-gamma, REG-III, Reg III-gamma, Reg3G, Regenerating islet-derived protein III-gamma, UNQ429, regenerating gene III, regenerating islet-derived 3 gamma, regenerating islet-derived protein 3-gamma
Citations for Recombinant Rat Reg3G Protein, CF
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Product Documents for Recombinant Rat Reg3G Protein, CF
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