Recombinant Rat PDGF-AB Protein, CF

Name: Recombinant Rat PDGF-AB Protein, CF
Brand: R&D Systems
SKU: 1115-AB

Catalog # 1115-AB | R&D Systems, Inc. a Bio-Techne Brand

Citations (2)

Product Datasheet / COA / SDS

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Key Product Details

Source:	E. coli
Accession #:	AAB59693.1 (A chain) & Q05028.1 (B chain)
Structure / Form:	Disulfide-linked heterodimer
Applications:	Bioactivity

View Full Technical Details for Recombinant Rat PDGF-AB Protein, CF

Technical Data
Citations (2) / Reviews

Product Specifications for Recombinant Rat PDGF-AB Protein, CF

Source

E. coli-derived rat PDGF-AB protein

Rat PDGF-A
(Ser87 - Arg196)
Accession # AAB59693.1

Rat PDGF-B (Ser74 - Thr182) Accession # Q05028.1
N-terminus	C-terminus

Purity

>97%, by SDS-PAGE under reducing conditions and visualized by silver stain.

Endotoxin level

<0.10 EU per 1 μg of the protein by the LAL method.

N-terminal sequence Analysis

Ser87 (A chain) & Ser74 (B chain)

Predicted Molecular Mass

24.8 kDa

SDS-PAGE

24.8 kDa

Activity

Measured in a cell proliferation assay using NR6R‑3T3 mouse fibroblast cells. Raines, E.W. et al. (1985) Methods Enzymol. 109:749. The ED₅₀ for this effect is 1-3 ng/mL.

Formulation, Preparation and Storage

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

1115-AB

Formulation	Lyophilized from a 0.2 μm filtered solution in Acetonitrile and Na2HPO₄.
Reconstitution	Reconstitute at 100 μg/mL in sterile 4 mM HCl. Reconstitution Buffer Available: Size / Price Qty Loading... RB03:
Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage:	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: PDGF-AB

Platelet-derived growth factor (PDGF) family of mesenchymal mitogenic factors consists of four homodimers (PDGF-AA, PDGF-BB, PDGF-CC and PDGF-DD) and one heterodimer (PDGF-AB). These proteins are expressed by a number of different cells and tissues including endothelial cells, epithelial cells, hematopoietic cells, connective tissue, nervous tissue, brain, muscle, kidney and liver. PDGF expression is highly regulated with PDGF levels increasing following injury and/or disease. An analysis of PDGF purified from human platelets suggest that approximately 70% of sera-derived PDGF is PDGF-AB (1).

PDGF isoforms exert their cellular effect by binding to the structurally similar receptor tyrosine kinases PDGF receptor-alpha and PDGF receptor-beta. PDGF-AB binding induces receptor dimerization resulting in alpha alpha receptor homodimers and alpha beta receptor heterodimers. Binding of PDGF receptors has been reported to result in stimulation of mitogenicity and chemotaxis of fibroblasts, stimulation of granule release by neutrophils and monocytes, facilitation of steroid synthesis by Leydig cells, stimulation of neutrophil phagocytosis, stimulation of collagen, fibronectin, proteoglycan, and hyaluronic acid synthesis, modulation of thrombospondin expression and secretion, stimulation of collagenase activity and secretion, induction of contraction of rat aorta strips in vitro, and transient induction of T cell IL-2 secretion accompanied by a down-regulation of IL-4 and IFN-gamma production (2).

Item	Value
References	Raines, E.W. (2001) in Cytokine Reference, J.J. Oppenheim and M. Feldmann, chief editors, Academic Press, San Diego, California, pp. 755. Heldin, C-H, Eriksson, U. and Ostman, A. (2002) Arch. Biochem. Biophysic. 398:284.
Long Name	Platelet-derived Growth Factor AB
Alternate Names	PDGF-AB, PDGFAB

Citations for Recombinant Rat PDGF-AB Protein, CF (2)

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Citations are publications that use Bio-Techne products. Selected citations for Recombinant Rat PDGF-AB Protein, CF include:

Species: Rat
Sample Types: Whole Cells
Applications: Bioassay

JL Kadrmas et al. (2020-11-09), Genetic analyses in mouse fibroblast and melanoma cells demonstrate novel roles for PDGF-AB ligand and PDGF receptor alpha Sci Rep, 2020-110(1):19303.
PMID: 33168840
Species: Rat
Sample Types: Whole Cells
Applications: Bioassay

X Sun et al. (2018-05-11), Differentiation of adipose-derived stem cells into Schwann cell-like cells through intermittent induction: potential advantage of cellular transient memory function Stem Cell Res Ther, 2018-09(1):133.
PMID: 29751848

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Product Documents for Recombinant Rat PDGF-AB Protein, CF

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