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Recombinant Rat Nogo-A Fc Chimera Protein, CF

Catalog # 2445-NG | R&D Systems, Inc. a Bio-Techne Brand
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2445-NG-050

Key Product Details

Source

NS0

Accession #

Structure / Form

Disulfide-linked homodimer

Conjugate

Unconjugated

Applications

Bioactivity

Product Specifications

Source

Mouse myeloma cell line, NS0-derived rat Nogo-A protein
Rat Nogo-A
(Thr544 - Phe725)
Accession # Q9JK11
IEGRMD Human IgG1
(Pro100 - Lys330)
N-terminus C-terminus

Purity

>95%, by SDS-PAGE under reducing conditions and visualized by silver stain.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

Thr544

Predicted Molecular Mass

46.1 kDa (monomer)

SDS-PAGE

75-85 kDa, reducing conditions

Activity

Measured by its ability to inhibit neurite outgrowth of dissociated E13 chick embryonic dorsal root ganglia (DRG) neurons.
Able to significantly inhibit neurite outgrowth when immobilized as a 3 µL droplet containing 300 ng on a nitrocellulose-coated microplate.

Formulation, Preparation and Storage

2445-NG
Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution
Reconstitute at 100 μg/mL in sterile PBS.

Reconstitution Buffer Available:
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Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Nogo-A

Rat Nogo-A is a member of the reticulon family of transmembrane proteins. This family is characterized by the presence of a nonsignal sequence-containing N-terminus, a topologically conserved approximately 200 amino acid (aa) C-terminus that contains two transmembrane domains and an ER-retention motif, and a punctate intracellular distribution within the ER that is reminescent of a reticulum (1, 2). Nogo-A in rat exists in four isoforms (3 - 5). The full length rat Nogo-A is 1163 aa in length and contains a 989 aa N-terminus, a 21 aa transmembrane segment, a 94 aa connecting “loop”, a second 21 aa transmembrane segment, and a 38 aa C-terminus. Three areas are of particular interest. One is a stretch of 66 aa within the 94 aa transmembrane connecting loop (SWISSPROT defines this region as being 94 aa in length while the original cloning papers identified it as being 66 aa in length). This segment is reported to bind to the GPI-linked Nogo receptor/p75 complex on axons and induce growth cone collapse (6 - 8). Two other areas in the N-terminus have also been discovered to have bioactivity (6, 9, 10). The amino acid segment 59 - 172 is reported to block fibroblast spreading, while the aa segment 544 - 725 blocks neurite outgrowth and block fibroblast spreading (6, 10). The exact topology of Nogo-A is unclear. With two transmembrane segments, the N- and C-termini may be extracellular with the “loop” region intracellular, or the situation could be reversed (11, 12). Alternatively, the loop region and N-terminus may be on the same side of the membrane (6). Nogo-A is expressed in neurons, endothelial cells. oligodendrocytes, fibroblasts and myoblasts (10, 13, 14). Rat Nogo-A is 78% aa identical to human Nogo-A overall, with 98% aa identical in the loop region and 81% aa identity in the aa 544 - 725 segment.

References

  1. Oertle, T. et al. (2003) FASEB J. 17:1238.
  2. GrandPre, T. et al. (2000) Nature 403:439.
  3. Chen, M.S. et al. (2000) Nature 403:434.
  4. Morris, N.J. et al. (1999) Biochim. Biophys. Acta 1450:68.
  5. Ito, T. and S.M. Schwartz (1999) GenBank Accession # Q9JK11.
  6. Oertle, T. et al. (2003) J. Neurosci. 23:5393.
  7. Fournier, A.E. et al. (2001) Nature 409:341.
  8. Wang, K.C. et al. (2002) Nature 420:74.
  9. Prinjha, R. et al. (2000) Nature 403:383.
  10. Dodd, D.A. et al. (2005) J. Biol. Chem. 280:12494.
  11. Huber, A.B. and M.E. Schwab (2000) Biol. Chem. 381:407.
  12. Ng, C.E.L. and B.L. Tang (2002) J. Neurosci. Res. 67:559.
  13. Wang, X. et al. (2002) J. Neurosci. 22:5505.
  14. Acevedo, L. et al. (2004) Nat. Med. 10:382.

Long Name

Reticulon 4A

Alternate Names

NI220, NogoA, RTN4, RTN4A

Entrez Gene IDs

57142 (Human); 68585 (Mouse); 83765 (Rat)

Gene Symbol

RTN4

UniProt

Product Documents for Recombinant Rat Nogo-A Fc Chimera Protein, CF

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

Product Specific Notices for Recombinant Rat Nogo-A Fc Chimera Protein, CF

For research use only

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