Recombinant Rat Lipocalin-2/NGAL Protein, CF

Name: Recombinant Rat Lipocalin-2/NGAL Protein, CF
Brand: R&D Systems
SKU: 3508-LC
Price: 417 USD

Catalog # 3508-LC | R&D Systems, Inc. a Bio-Techne Brand

Citations (3)

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Key Product Details

Source:	NS0
Accession #:	P30152
Applications:	Binding Activity

View Full Technical Details for Recombinant Rat Lipocalin-2/NGAL Protein, CF

Product Specifications for Recombinant Rat Lipocalin-2/NGAL Protein, CF

Source	Mouse myeloma cell line, NS0-derived rat Lipocalin-2/NGAL protein Gln21-Asn198, with a C-terminal 6-His tag
Purity	>95%, by SDS-PAGE under reducing conditions and visualized by silver stain
Endotoxin level	<1.0 EU per 1 μg of the protein by the LAL method.
N-terminal sequence Analysis	No results obtained: Gln21 predicted
Predicted Molecular Mass	21 kDa
SDS-PAGE	27 kDa, reducing conditions
Activity	Measured by its ability to bind Iron(III) dihydroxybenzoic acid [Fe(DHBA)₃]. The binding of Fe(DHBA)₃ results in the quenching of Trp fluorescence in Lipocalin-2. Recombinant Rat Lipocalin‑2/NGAL can bind >1.0 µM of Fe(DHBA)₃, as measured under the described conditions.

Formulation, Preparation and Storage

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

3508-LC

Formulation	Lyophilized from a 0.2 μm filtered solution in MES and NaCl.
Reconstitution	Reconstitute at 500 μg/mL in sterile 25 mM MES and 150 mM NaCl, pH 6.5.
Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage:	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 6 months from date of receipt, -20 to -70 °C as supplied. 3 months, -20 to -70 °C under sterile conditions after reconstitution.

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Background: Lipocalin-2/NGAL

Lipocalin-2, also known as neutrophil gelatinase-associated Lipocalin and uterocalin (NGAL), has been implicated in a variety of processes including cell differentiation, tumorigenesis, and apoptosis (1‑3). It binds a bacterial catecholate sidropore bound to ferric ion such as enterobactin with a subnanomolar dissociation constant (K_D= 0.41 nM) (4). The bound ferric enterobactin complex breaks down slowly in a month into dihydroxybenzoyl serine and dihydroxybenzoic acid (DHBA). It also binds to a ferric DHBA complex with much less K_D values (7.9 nM) (4). Secretion of Lipocalin‑2 in immune cells increases by stimulation of Toll‑like receptor as a acute phase response to infection. As a result, it acts as a potent bacteriostatic reagents by sequestering iron (5). Moreover, Lipocalin‑2 can alter the invasive and metastatic behavior of Ras‑transformed breast cancer cells in vitro and in vivo by reversing epithelial to mesenchymal transition inducing activity of Ras, through restoration of E‑cadherin expression, via effects on the Ras‑MAPK signaling pathway (6). In the kidney, Lipocalin‑2‑mediated iron trafficking may be involved in protection from renal injury, and it has been implicated as a marker for early kidney failure (7, 8).

Item	Value
References	Kjeldsen L, et al. (2000) Biochim Biophys Acta. 1482:272. Devireddy, L.R. et al. (2001) Science 293:829. Yang, M.B. et al. (2002) Mol. Cell. 10:1045. Goetz, D.H. et al. (2002) Mol. Cell 10:1033. Flo, T.H. et al. (2004) Nature 432:917. Hanai, J. et al. (2005) J. Biol. Chem. 280:13641. Mori, K. et al. (2005) J. Clin. Invest. 115:610. Mishra, J. et al. (2005) Lancet 365:1231.
Long Name	Neutrophil Gelatinase-associated Lipocalin
Entrez Gene IDs	3934 (Human); 16819 (Mouse); 170496 (Rat); 102137149 (Cynomolgus Monkey)
Alternate Names	24p3, 25 kDa alpha-2-microglobulin-related subunit of MMP-9, HNL, LCN2, Lipocalin-2, Lipocalin2, MSFI, NGAL, NGALlipocalin-2, Oncogene 24p3, Siderocalin, Uterocalin, lipocalin 2, lipocalin 2 (oncogene 24p3), migration-stimulating factor inhibitor, neutrophil gelatinase-associated lipocalin, p25

Citations for Recombinant Rat Lipocalin-2/NGAL Protein, CF (3)

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Citations are publications that use Bio-Techne products. Selected citations for Recombinant Rat Lipocalin-2/NGAL Protein, CF include:

Species: Rat
Sample Types: In Vivo
Applications: In Vivo

W Tang et al. (2018-03-23), Lipocalin 2 Suppresses Ocular Inflammation by Inhibiting the Activation of NF-?? Pathway in Endotoxin-Induced Uveitis Cell. Physiol. Biochem., 2018-046(1):375-388.
PMID: 29590655
Species: Rat
Sample Types: Whole Cells
Applications: Bioassay

Chia W et al. (2015-05-21), Expression and localisation of brain-type organic cation transporter (BOCT/24p3R/LCN2R) in the normal rat hippocampus and after kainate-induced excitotoxicity. Neurochem Int, 2015-087(0):43-59.
PMID: 26004810
Sample Types: N/A
Applications: ELISA (Standard)

Soler-Garcia AA et al. (2009-03-11), Iron-related proteins: candidate urine biomarkers in childhood HIV-associated renal diseases. Clin J Am Soc Nephrol, 2009-04(4):763-71.
PMID: 19279121

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