Recombinant Rat IL-13 Protein

Name: Recombinant Rat IL-13 Protein
Brand: R&D Systems
SKU: 1945-RL
Rating: 5 (2 reviews)

Catalog # 1945-RL | R&D Systems, Inc. a Bio-Techne Brand

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Citations (5)

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Key Product Details

Source:	E. coli
Accession #:	P42203
Applications:	Bioactivity

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Product Specifications for Recombinant Rat IL-13 Protein

Source	E. coli-derived rat IL-13 protein Thr19-His131
Purity	>97%, by SDS-PAGE under reducing conditions and visualized by silver stain
Endotoxin level	<0.10 EU per 1 μg of the protein by the LAL method.
N-terminal sequence Analysis	Thr19
Predicted Molecular Mass	12.3 kDa
Activity	Measured in a cell proliferation assay using TF‑1 human erythroleukemic cells. Kitamura, T. et al. (1989) J. Cell Physiol. 140:323. The ED₅₀ for this effect is 1-4 ng/mL.

Formulation, Preparation and Storage

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

1945-RL

Carrier

Formulation	Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.
Reconstitution	Reconstitute at 10 μg/mL in sterile PBS containing at least 0.1% human or bovine serum albumin.
Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage:	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 3 months, -20 to -70 °C under sterile conditions after reconstitution.

1945-RL/CF

Carrier Free

Formulation	Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution	Reconstitute at 100 μg/mL in sterile PBS.
Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage:	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: IL-13

IL-13 is a 17 kDa immunoregulatory cytokine that plays a key role in the pathogenesis of allergic asthma and atopy. It is secreted by Th1 and Th2 CD4⁺ T cells, NK cells, visceral smooth muscle cells, eosinophils, mast cells, and basophils (1 - 3). IL-13 circulates as a monomer with two internal disulfide bonds that contribute to a bundled four alpha -helix configuration (4, 5). Mature rat IL-13 shares 59%, 75%, and 60% amino acid sequence identity with human, mouse, and rhesus IL-13, respectively. Despite the low homology, it exhibits cross-species activity between human, mouse, and rat (6, 7). IL-13 has diverse activities on numerous cell types (8). On macrophages, IL-13 suppresses the production of proinflammatory cytokines and other cytotoxic substances. On B cells, IL-13 induces immunoglobulin class switching to IgE, upregulates the expression of MHC class II, CD71, CD72, and CD23, and costimulates proliferation. IL-13 upregulates IL-6 while downregulating IL-1 and TNF-alpha production by fibroblasts and endothelial cells. IL-13 binds with low affinity to IL-13 R alpha 1, triggering IL-13 R alpha 1 association with IL-4 R alpha. This high affinity receptor complex also functions as the type 2 IL-4 receptor complex (9, 10). Additionally, IL-13 binds with high affinity to IL-13 R alpha 2 which is expressed intracellularly, on the cell surface, and as a soluble molecule (11 - 14). IL-13 R alpha 2 regulates the bioavailability of both IL-13 and IL-4 and is overexpressed in glioma and several bronchial pathologies (10, 15, 16). Compared to wild type IL-13, the atopy-associated R110Q variant of IL-13 elicits increased responsiveness from eosinophils that express low levels of IL-13 R alpha 2 (17).

Item	Value
References	Wills-Karp, M. (2004) Immunol. Rev. 202:175. Nakajima H. and K. Takatsu (2007) Int. Arch. Allergy Immunol. 142:265. Lakkis, F.G and E.N. Cruet (1993) Biochem. Biophys. Res. Commun. 197:612. Moy, F.J. et al. (2001) J. Mol. Biol. 310:219. Eisenmesser, E.Z. et al. (2001) J. Mol. Biol. 310:231. Ruetten, H. and C. Thiemermann (1997) Shock 8:409. Lakkis, F.G. et al. (1997) Biochem. Biophys. Res. Commun. 235:529. Wynn, T.A. (2003) Annu. Rev. Immunol. 21:425. Andrews, A.L. et al. (2002) J. Biol. Chem. 277:46073. Tabata, Y. et al. (2007) Curr. Allergy Asthma Rep. 7:338. Chiaramonte, M.G. et al. (2003) J. Exp. Med. 197:687. Daines, M.O. and G.K. Hershey (2002) J. Biol. Chem. 227:10387 Matsumura, M. et al. (2007) Biochem. Biophys. Res. Commun.360:464. Tabata, Y. et al. (2007) J. Immunol. 177:7905. Andrews, A.L. et al. (2006) J. Allergy Clin. Immunol. 118:858. Joshi, B.H. et al. (2006) Vitam. Horm. 74:479. Andrews, A.-L. et al. (2007) J. Allergy Clin. Immunol. 120:91.
Long Name	Interleukin 13
Entrez Gene IDs	3596 (Human); 16163 (Mouse); 116553 (Rat); 574325 (Primate)
Alternate Names	ALRHMGC116789, BHR1interleukin-13, IL-13, IL-13MGC116788, IL13, MGC116786, NC30, P600, interleukin 13

Citations for Recombinant Rat IL-13 Protein (5)

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Citations are publications that use Bio-Techne products. Selected citations for Recombinant Rat IL-13 Protein include:

Species: Rat
Sample Types: Tissue Explant
Applications: Bioassay

CJ Lawrimore et al. (2019-09-10), Ethanol Induction of Innate Immune Signals Across BV2 Microglia and SH-SY5Y Neuroblastoma Involves Induction of IL-4 and IL-13 Brain Sci, 2019-09(9).
PMID: 31510019
Species: Rat
Sample Types: Whole Cells
Applications: Bioassay

JE Lim et al. (2017-08-25), A neuropeptide, Substance-P, directly induces tissue-repairing M2 like macrophages by activating the PI3K/Akt/mTOR pathway even in the presence of IFN? Sci Rep, 2017-07(1):9417.
PMID: 28842601
Species: Rat
Sample Types: Whole Cells
Applications: Bioassay

Steelman A et al. (2013-07-08), Galectin-9 protein is up-regulated in astrocytes by tumor necrosis factor and promotes encephalitogenic T-cell apoptosis. J Biol Chem, 2013-0288(33):23776-87.
PMID: 23836896
Species: Rat
Sample Types: Whole Cells
Applications: Bioassay

Krejci P et al. (2008-10-12), Fibroblast growth factor inhibits interferon gamma-STAT1 and interleukin 6-STAT3 signaling in chondrocytes. Cell. Signal., 2008-121(1):151-60.
PMID: 18950705
Species: Human
Sample Types: Whole Cells
Applications: Bioassay

Miller R et al. (2008-03-07), Development of an in vitro potency bioassay for therapeutic IL-13 antagonists: the A-549 cell bioassay. J. Immunol. Methods, 2008-0334(1):134-41.
PMID: 18355834

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Customer Reviews for Recombinant Rat IL-13 Protein (2)

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Recombinant Rat IL-13 Protein

Name: Anonymous

Application: In vitro bioactivity in cell culture

Reason for Rating: Observed good biological activity in vitro.

Verified Customer | Posted 11/20/2021
Recombinant Rat IL-13 Protein

Name: Brad Morrison

Application: Enzymatic activity in vitro

Reason for Rating: Worked like a charm for stimulating an IL-13Ra1 signaling reporter cell line (293T).

Verified Customer | Posted 01/15/2016

Added IL-13 10ng/mL and 100ng/mL to full culture media overnight and measured secreted reporter the next day.

Recombinant Rat IL-13 Protein

Catalog # 1945-RL | R&D Systems, Inc. a Bio-Techne Brand

Key Product Details

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Product Specifications for Recombinant Rat IL-13 Protein

Formulation, Preparation and Storage

Carrier Free

1945-RL

Carrier

1945-RL/CF

Carrier Free

Reconstitution Calculator

Background: IL-13

Citations for Recombinant Rat IL-13 Protein (5)

Customer Reviews for Recombinant Rat IL-13 Protein (2)

Recombinant Rat IL-13 Protein

Recombinant Rat IL-13 Protein

Product Documents for Recombinant Rat IL-13 Protein

Product Datasheets

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