Recombinant Rat IFN-gamma Protein

Name: Recombinant Rat IFN-gamma Protein
Brand: R&D Systems
SKU: 585-IF
Rating: 5 (4 reviews)

Catalog # 585-IF | R&D Systems, Inc. a Bio-Techne Brand

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Citations (38)

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Setting the standard in quality research reagents

Key Product Details

Source:	E. coli
Accession #:	P01581
Applications:	Bioactivity

View Full Technical Details for Recombinant Rat IFN-gamma Protein

Technical Data
Citations (38) / Reviews (4)

Product Specifications for Recombinant Rat IFN-gamma Protein

Source	E. coli-derived rat IFN-gamma protein Glu23-Cys156, with an N-terminal Met
Purity	>97%, by SDS-PAGE under reducing conditions and visualized by silver stain
Endotoxin level	<0.01 EU per 1 μg of the protein by the LAL method.
N-terminal sequence Analysis	Met
Predicted Molecular Mass	15.5 kDa
SDS-PAGE	15.5 kDa
Activity	Measured in an anti-viral assay using L‑929 mouse fibroblast cells infected with encephalomyocarditis (EMC) virus. Meager, A. (1987) in Lymphokines and Interferons, a Practical Approach. Clemens, M.J. et al. (eds): IRL Press. 129. The ED₅₀ for this effect is 0.1‑0.6 ng/mL.

Formulation, Preparation and Storage

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

585-IF

Carrier

Formulation	Lyophilized from a 0.2 μm filtered solution in Tris, NaCl and PEG with BSA as a carrier protein.
Reconstitution	Reconstitute at 100 μg/mL in sterile PBS containing at least 0.1% human or bovine serum albumin.
Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage:	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 3 months, -20 to -70 °C under sterile conditions after reconstitution.

585-IF/CF

Carrier Free

Formulation	Supplied as a 0.2 μm filtered solution in Tris, NaCl and PEG 3350.
Shipping	The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage:	3 months from date of receipt, 2 to 8 °C as supplied.

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: IFN-gamma

Interferon-gamma (IFN-gamma ), also known as type II or immune interferon, exerts a wide range of immunoregulatory activities and is considered to be the prototype proinflammatory cytokine (1, 2). Mature rat IFN-gamma exists as a noncovalently linked homodimer of 20 - 25 kDa variably glycosylated subunits (3). It shares 86% amino acid sequence identity with mouse IFN-gamma and 37%‑45% with bovine, canine, cotton rat, equine, feline, human, porcine, and rhesus IFN-gamma. IFN-gamma dimers bind to IFN-gamma RI (alpha subunits) which then interact with IFN-gamma RII (beta subunits) to form the functional receptor complex of two alpha and two beta subunits. Inclusion of IFN-gamma RII increases the binding affinity for ligand and the efficiency of signal transduction (4, 5). IFN‑ gamma is produced by a variety of immune cells under inflammatory conditions, notably by T cells and NK cells (6). It plays a key role in host defense by promoting the development and activation of Th1 cells, chemoattraction and activation of monocytes and macrophages, upregulation of antigen presentation molecules, and immunoglobulin class switching in B cells. It also exhibits antiviral, antiproliferative, and apoptotic effects (6, 7). In addition, IFN-gamma functions as an anti-inflammatory mediator by promoting the development of regulatory T cells and inhibiting Th17 cell differentiation (8, 9). The pleiotropic effects of IFN-gamma contribute to the development of multiple aspects of atherosclerosis (7).

Item	Value
References	Billiau, A. and P. Matthys (2009) Cytokine Growth Factor Rev. 20:97. Pestka, S. et al. (2004) Immunol. Rev. 202:8. Dijkema, R. et al. (1985) EMBO J. 4:761. Marsters, S.A. et al. (1995) Proc. Natl. Acad. Sci. 92:5401. Krause, C.D. et al. (2000) J. Biol. Chem. 275:22995. Schroder, K. et al. (2004) J. Leukoc. Biol. 75:163. McLaren, J.E. and D.P. Ramji (2009) Cytokine Growth Factor Rev. 20:125. Muhl, H. and J. Pfeilschifter (2003) Int. Immunopharmacol. 3:1247. Kelchtermans, H. et al. (2008) Trends Immunol. 29:479.
Long Name	Interferon gamma
Entrez Gene IDs	3458 (Human); 15978 (Mouse); 25712 (Rat); 396991 (Porcine); 281237 (Bovine); 403801 (Canine); 493965 (Feline)
Alternate Names	IFG, IFI, IFN-gamma, IFNG, IFNgamma, Immune interferon, interferon gamma, interferon, gamma

Citations for Recombinant Rat IFN-gamma Protein (38)

Showing 1 - 10 of 38 citations Showing All

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Citations are publications that use Bio-Techne products. Selected citations for Recombinant Rat IFN-gamma Protein include:

Species: Rat
Sample Types: Whole Cells
Applications: Bioassay

AA Perez-Sern et al. (2023-03-16), BCL-XL Overexpression Protects Pancreatic beta-Cells against Cytokine- and Palmitate-Induced Apoptosis International Journal of Molecular Sciences, 2023-024(6).
PMID: 36982731
Species: Mouse
Sample Types: Whole Cells
Applications: Bioassay

A Badia-Sote et al. (2022-04-22), High-Throughput Analysis of Astrocyte Cultures Shows Prevention of Reactive Astrogliosis by the Multi-Nutrient Combination Fortasyn Connect Cells, 2022-011(9).
PMID: 35563732
Species: Mouse
Sample Types: Whole Cells
Applications: Stimulation

N Javeed et al. (2021-08-24), Pro-inflammatory beta cell small extracellular vesicles induce beta cell failure through activation of the CXCL10/CXCR3 axis in diabetes Cell Reports, 2021-036(8):109613.
PMID: 34433033
Species: Rat
Sample Types: Whole Cells
Applications: Bioassay

S Kim et al. (2021-08-16), Astrocytes Stimulate Microglial Proliferation and M2 Polarization In Vitro through Crosstalk between Astrocytes and Microglia International Journal of Molecular Sciences, 2021-022(16).
PMID: 34445510
Species: Rat
Sample Types: Whole Cells
Applications: Bioassay

KM Pusic et al. (2021-08-13), IFNgamma-stimulated dendritic cell extracellular vesicles can be nasally administered to the brain and enter oligodendrocytes PLoS ONE, 2021-016(8):e0255778.
PMID: 34388189
Species: Rat
Sample Types: Whole Cells
Applications: Bioassay

Y Wang et al. (2020-10-16), Genome-wide screens identify Toxoplasma gondii determinants of parasite fitness in IFN&gamma-activated murine macrophages Nat Commun, 2020-111(1):5258.
PMID: 33067458
Species: Rat
Sample Types: Whole Cells
Applications: Bioassay

S Vig et al. (2019-04-05), Cytokine-induced translocation of GRP78 to the plasma membrane triggers a pro-apoptotic feedback loop in pancreatic beta cells Cell Death Dis, 2019-010(4):309.
PMID: 30952835
Species: Rat
Sample Types: Whole Cells
Applications: Bioassay

S Lively et al. (2018-07-24), Microglia Responses to Pro-inflammatory Stimuli (LPS, IFN?+TNF?) and Reprogramming by Resolving Cytokines (IL-4, IL-10) Front Cell Neurosci, 2018-012(0):215.
PMID: 30087595
Species: Rat
Sample Types: Whole Cells
Applications: Bioassay

TR Nardelli et al. (2018-04-09), Prolactin protects against cytokine-induced beta cell death by NFkB and JNK inhibition J. Mol. Endocrinol., 2018-00(0).
PMID: 29632026
Species: Rat
Sample Types: Serum
Applications: ELISA (Standard)

T Erkens et al. (2018-01-31), Analytical performance of a commercial multiplex Luminex-based cytokine panel in the rat J Pharmacol Toxicol Methods, 2018-091(0):43-49.
PMID: 29371052
Species: Rat
Sample Types: Whole Cells
Applications: Bioassay

M Lambelet et al. (2018-01-24), Dysfunctional autophagy following exposure to pro-inflammatory cytokines contributes to pancreatic ?-cell apoptosis Cell Death Dis, 2018-09(2):96.
PMID: 29367588
Species: Rat
Sample Types: Whole Cells
Applications: Bioassay

JE Lim et al. (2017-08-25), A neuropeptide, Substance-P, directly induces tissue-repairing M2 like macrophages by activating the PI3K/Akt/mTOR pathway even in the presence of IFN? Sci Rep, 2017-07(1):9417.
PMID: 28842601
Species: Rat
Sample Types: Whole Cells
Applications: Bioassay

AL Clark et al. (2017-07-17), Targeting Cellular Calcium Homeostasis to Prevent Cytokine-Mediated Beta Cell Death Sci Rep, 2017-07(1):5611.
PMID: 28717166
Species: Rat
Sample Types: Whole Cells
Applications: Bioassay

DA Cunha et al. (2017-07-11), Pancreatic ?-cell protection from inflammatory stress by the endoplasmic reticulum proteins thrombospondin 1 and mesencephalic astrocyte-derived neutrotrophic factor (MANF) J. Biol. Chem., 2017-00(0).
PMID: 28698383
Species: Rat
Sample Types: In Vivo
Applications: In Vivo

O Stojadinov et al. (2017-05-30), Wound healing protects against chemotherapy-induced alopecia in young rats via up-regulating interleukin-1?-mediated signaling Heliyon, 2017-03(5):e00309.
PMID: 28607955
Species: Rat
Sample Types: Whole Cells
Applications: Bioassay

L Cambier et al. (2017-03-01), Y RNA fragment in extracellular vesicles confers cardioprotection via modulation of IL-10 expression and secretion EMBO Mol Med, 2017-09(3):337-352.
PMID: 28167565
Species: Rat
Sample Types: Whole Cells
Applications: Bioassay

F Brozzi et al. (2016-04-04), Ubiquitin D regulates IRE1?/JNK-dependent apoptosis in pancreatic beta cells J Biol Chem, 2016-00(0).
PMID: 27044747
Species: Rat
Sample Types: Whole Cells
Applications: Bioassay

Marroquin Belaunzaran O et al. (2015-06-30), HLA-B27-Homodimer-Specific Antibody Modulates the Expansion of Pro-Inflammatory T-Cells in HLA-B27 Transgenic Rats. PLoS ONE, 2015-010(6):e0130811.
PMID: 26125554
Species: Rat
Sample Types: Whole Cells
Applications: Bioassay

Miljkovic D et al. (2015-02-13), A comparative analysis of multiple sclerosis-relevant anti-inflammatory properties of ethyl pyruvate and dimethyl fumarate. J Immunol, 2015-0194(6):2493-503.
PMID: 25681336
Species: Rat
Sample Types: Whole Cells
Applications: Bioassay

Lazzaro M et al. (2014-09-16), Ceruloplasmin potentiates nitric oxide synthase activity and cytokine secretion in activated microglia. J Neuroinflammation, 2014-011(0):164.
PMID: 25224679
Species: Mouse
Sample Types: Whole Cells
Applications: Bioassay

Salzano S et al. (2014-08-05), Linkage of inflammation and oxidative stress via release of glutathionylated peroxiredoxin-2, which acts as a danger signal. Proc Natl Acad Sci U S A, 2014-0111(33):12157-62.
PMID: 25097261
Species: Rat
Sample Types: Whole Cells
Applications: Bioassay

Dahllof M et al. (2014-07-25), HDAC inhibitor-mediated beta-cell protection against cytokine-induced toxicity is STAT1 Tyr701 phosphorylation independent. J Interferon Cytokine Res, 2014-035(1):63-70.
PMID: 25062500
Species: Rat
Sample Types: Whole Cells
Applications: Bioassay

Brozzi F et al. (2014-07-25), A combined "omics" approach identifies N-Myc interactor as a novel cytokine-induced regulator of IRE1 protein and c-Jun N-terminal kinase in pancreatic beta cells. J Biol Chem, 2014-0289(30):20677-93.
PMID: 24936061
Species: Rat
Sample Types: Whole Cells
Applications: Bioassay

Marroqui L et al. (2014-03-07), BACH2, a candidate risk gene for type 1 diabetes, regulates apoptosis in pancreatic beta-cells via JNK1 modulation and crosstalk with the candidate gene PTPN2. Diabetes, 2014-063(7):2516-27.
PMID: 24608439
Species: Rat
Sample Types: Whole Cells
Applications: Bioassay

Backe M et al. (2014-01-01), Altering beta-cell number through stable alteration of miR-21 and miR-34a expression. Islets, 2014-06(1):e27754.
PMID: 25483877
Species: Rat
Sample Types: Whole Cells
Applications: Bioassay

Steelman A et al. (2013-07-08), Galectin-9 protein is up-regulated in astrocytes by tumor necrosis factor and promotes encephalitogenic T-cell apoptosis. J Biol Chem, 2013-0288(33):23776-87.
PMID: 23836896
Species: Rat
Sample Types: Whole Cells
Applications: Bioassay

Quan Y et al. (2013-02-12), EP2 receptor signaling pathways regulate classical activation of microglia. J Biol Chem, 2013-0288(13):9293-302.
PMID: 23404506
Species: Rat
Sample Types: Whole Cells
Applications: Bioassay

Weaver J et al. (2013-02-11), Regulation of NOX-1 expression in beta cells: a positive feedback loop involving the Src-kinase signaling pathway. Mol Cell Endocrinol, 2013-0369(1):35-41.
PMID: 23410839
Species: Rat
Sample Types: Whole Cells
Applications: Bioassay

Santin I et al. (2012-11-15), USP18 is a key regulator of the interferon-driven gene network modulating pancreatic beta cell inflammation and apoptosis. Cell Death Dis, 2012-13(0):e419.
PMID: 23152055
Species: Rat
Sample Types: Whole Cells
Applications: Bioassay

Moore F et al. (2010-10-27), STAT1 is a master regulator of pancreatic {beta}-cell apoptosis and islet inflammation. J. Biol. Chem., 2010-1286(2):929-41.
PMID: 20980260
Species: Rat
Sample Types: Whole Cells
Applications: Bioassay

Schwarznau A et al. (2009-08-01), IL-1beta receptor blockade protects islets against pro-inflammatory cytokine induced necrosis and apoptosis. J. Cell. Physiol., 2009-0220(2):341-7.
PMID: 19334038
Species: Rat
Sample Types: Whole Cells
Applications: Bioassay

Husberg C et al. (2008-05-28), Cytokine expression profiling of the myocardium reveals a role for CX3CL1 (fractalkine) in heart failure. J. Mol. Cell. Cardiol., 2008-045(2):261-9.
PMID: 18585734
Species: Rat
Sample Types: Whole Cells
Applications: Bioassay

Paintlia AS et al. (2008-01-31), Inhibition of rho family functions by lovastatin promotes myelin repair in ameliorating experimental autoimmune encephalomyelitis. Mol. Pharmacol., 2008-073(5):1381-93.
PMID: 18239032
Species: Rat
Sample Types: Whole Cells
Applications: Bioassay

Schell JB et al. (2007-08-15), Differential ex vivo nitric oxide production by acutely isolated neonatal and adult microglia. J. Neuroimmunol., 2007-0189(1):75-87.
PMID: 17698208
Species: Rat
Sample Types: N/A, Whole Tissue
Applications: ELISA (Standard)

Roche M et al. (2006-09-29), In vivo modulation of LPS-induced alterations in brain and peripheral cytokines and HPA axis activity by cannabinoids. J. Neuroimmunol., 2006-0181(1):57-67.
PMID: 17011047
Species: Rat
Sample Types: In Vivo
Applications: In Vivo

Rhodes KE et al. (2006-04-21), The injury response of oligodendrocyte precursor cells is induced by platelets, macrophages and inflammation-associated cytokines. Neuroscience, 2006-0140(1):87-100.
PMID: 16631314
Species: Rat
Sample Types: Whole Cells
Applications: Bioassay

Butovsky O et al. (2006-03-23), Induction and blockage of oligodendrogenesis by differently activated microglia in an animal model of multiple sclerosis. J. Clin. Invest., 2006-0116(4):905-15.
PMID: 16557302
Species: Rat
Sample Types: In Vivo
Applications: In Vivo

Frewert S et al. (2004-07-08), Intratumoral infusion of interleukin-1beta and interferon-gamma induces tumor invasion with macrophages and lymphocytes in a rat glioma model. Neurosci. Lett., 2004-0364(3):145-8.
PMID: 15196664

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Recombinant Rat IFN-gamma Protein

Name: Anonymous

Application: Immunoassay Standard

Verified Customer | Posted 05/24/2022
Recombinant Rat IFN-gamma Protein

Name: Anonymous

Application: In vitro bioactivity in cell culture

Verified Customer | Posted 05/19/2021
Recombinant Rat IFN-gamma Protein

Name: Leslie Priddy

Application: In vitro bioactivity in cell culture

Verified Customer | Posted 04/03/2018
Recombinant Rat IFN-gamma Protein

Name: Ella Zeldich

Application: In vitro bioactivity in cell culture

Reason for Rating: Works well and consistantly

Verified Customer | Posted 12/11/2017

Recombinant Rat IFN-gamma Protein

Catalog # 585-IF | R&D Systems, Inc. a Bio-Techne Brand

Key Product Details

Product Specifications for Recombinant Rat IFN-gamma Protein

Formulation, Preparation and Storage

Carrier Free

585-IF

Carrier

585-IF/CF

Carrier Free

Reconstitution Calculator

Background: IFN-gamma

Citations for Recombinant Rat IFN-gamma Protein (38)

Customer Reviews for Recombinant Rat IFN-gamma Protein (4)

Customer Images

Recombinant Rat IFN-gamma Protein

Recombinant Rat IFN-gamma Protein

Recombinant Rat IFN-gamma Protein

Recombinant Rat IFN-gamma Protein

Product Documents for Recombinant Rat IFN-gamma Protein

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