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Recombinant Rat FGF-BP Protein

Bio-Techne includes R&D Systems | Catalog # 1413-FB

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Carrier Free
1413-FB-025/CF

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With Carrier
1413-FB-025

Key Product Details

Source

NS0

Accession #

Conjugate

Unconjugated

Applications

Binding Activity

Product Specifications

Source

Mouse myeloma cell line, NS0-derived rat FGF-BP protein
Glu21-Cys238, with a C-terminal Asp and 10-His tag

Purity

>90%, by SDS-PAGE under reducing conditions and visualized by silver stain.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

Glu21

Predicted Molecular Mass

26 kDa

SDS-PAGE

Multiple bands between 25-37 kDa, reducing conditions

Activity

Measured by its binding ability in a functional ELISA.
Immobilized rhFGF-basic at 5 µg/mL (100 µL/well) can bind rrFGF-BP with a linear range of 0.8-50 ng/mL.

Formulation, Preparation and Storage

Carrier Free
What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

Carrier: 1413-FB
Formulation Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.
Reconstitution Reconstitute at 10 μg/mL in sterile PBS containing at least 0.1% human or bovine serum albumin.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Carrier Free: 1413-FB/CF
Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution Reconstitute at 100 μg/mL in sterile PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: FGF-BP

Fibroblast growth factor binding protein (FGF-BP), also known as HBp17, is a secreted glycoprotein that increases the bioavailability of FGFs (1). Mature FGF-BP is a 34 kDa O-glycosylated protein with five conserved intrachain disulfide bonds (2 - 4). FGF-BP contains a heparin-binding domain (aa 113 - 146) and a distinct FGF-binding region (aa 197 - 238) (5). Mature rat FGF-BP shares 54% and 81% aa sequence identity with mouse and rat FGF-BP, respectively. FGF-BP is expressed throughout development and in adult squamous epithelium (6, 7). It is upregulated in injured skin, renal tubular epithelium, and spinal nerves as well as in carcinomas of the skin, colon, and pancreas (3, 8 - 11). FGF-BP binds FGF -1, -2, -7, -10, and -22 which are secreted and sequestered in the extracellular matrix (ECM) (8, 12). The interactions of FGF-BP with heparin sulfate proteoglycans (HSPG) and FGF, modulates their activities (7, 9 13, 14). FGF-BP enhances the mitogenic effects of FGFs, thereby contributing to epithelial, endothelial, and neuronal tissue repair, angiogenesis, and tumor growth (8 - 10, 12, 15, 16).

 

References

  1. Abuharbeid, S. et al. (2006) Int. J. Biochem. Cell Biol. 38:1463.
  2. Aigner, A. et al. (2000) Growth Factors 18:51.
  3. Tassi, E. et al. (2006) Cancer Res. 66:1191.
  4. Lametsch, R. et al. (2000) J. Biol. Chem. 275:19469.
  5. Xie, B. et al. (2006) J. Biol. Chem. 281:1137.
  6. Aigner, A. et al. (2002) Histochem. Cell Biol. 117:1.
  7. Wu, D. et al. (1991) J. Biol. Chem. 266:16778.
  8. Beer, H.-D. et al. (2005) Oncogene 24:5269.
  9. Ray, P.E. et al. (2006) Am. J. Physiol. Regul. Integr. Comp. Physiol. 290:R105.
  10. Tassi, E. et al. (2007) Am. J. Physiol. Regul. Integr. Comp. Physiol. 293:R775.
  11. Kurtz, A. et al. (2004) Neoplasia 6:595.
  12. Tassi, E. et al. (2001) J. Biol. Chem. 276:40247.
  13. Mongiat, M. et al. (2001) J. Biol. Chem. 276:10263.
  14. Kurtz, A. et al. (1997) Oncogene 14:2671.
  15. Aigner, A. et al. (2001) Int. J. Cancer 92:510.
  16. Czubayko, F. et al. (1997) Nat. Med. 3:1137.

Long Name

Fibroblast Growth Factor Binding Protein

Alternate Names

FGF-BP1, FGFBP, FGFBP-1, FGFBP1, HBp17

Entrez Gene IDs

9982 (Human); 14181 (Mouse); 64535 (Rat)

Gene Symbol

FGFBP1

UniProt

Additional FGF-BP Products

Product Documents for Recombinant Rat FGF-BP Protein

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Note: Certificate of Analysis not available for kit components.

Product Specific Notices for Recombinant Rat FGF-BP Protein

For research use only

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Reconstitution Calculator

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