What receptors does FGF basic bind?

Name: Recombinant Rat FGF basic/FGF2/bFGF Protein
Brand: R&D Systems
SKU: 3339-FB

FGF receptor specificity has been reviewed in multiple citations. Please find more information at: //www.rndsystems.com/resources/articles/fibroblast-growth-factors-and-their-receptors

Recombinant Rat FGF basic/FGF2/bFGF Protein

R&D Systems, Inc. a Bio-Techne Brand

Catalog # 3339-FB

Citations (33)

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Source:	E. coli
Accession #:	P13109
Applications:	Bioactivity

View Full Technical Details for Recombinant Rat FGF basic/FGF2/bFGF Protein

Product Specifications for Recombinant Rat FGF basic/FGF2/bFGF Protein

Source	E. coli-derived rat FGF basic/FGF2/bFGF protein Ala11-Ser154
Purity	>95%, by SDS-PAGE under reducing conditions and visualized by silver stain.
Endotoxin level	<0.01 EU per 1 μg of the protein by the LAL method.
N-terminal sequence Analysis	Ala11
Predicted Molecular Mass	16.2 kDa
Activity	Measured in a cell proliferation assay using NR6R‑3T3 mouse fibroblast cells. Rizzino, A. et al. (1988) Cancer Res. 48:4266; Thomas, K. et al. (1987) Methods Enzymol. 147:120. The ED₅₀ for this effect is 0.3-1.8 ng/mL.

Formulation, Preparation and Storage

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

3339-FB

Carrier

Formulation	Lyophilized from a 0.2 μm filtered solution in MOPS, Na₂SO₄, EDTA and DTT with BSA as a carrier protein.
Reconstitution	Reconstitute at 100 μg/mL in sterile PBS containing at least 0.1% human or bovine serum albumin.
Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage:	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 3 months, -20 to -70 °C under sterile conditions after reconstitution.

3339-FB/CF

Carrier Free

Formulation	Lyophilized from a 0.2 μm filtered solution in MOPS, Na₂SO₄, EDTA and DTT.
Reconstitution	Reconstitute at 100 μg/mL in sterile PBS.
Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage:	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 3 months, -20 to -70 °C under sterile conditions after reconstitution.

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The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: FGF basic/FGF2/bFGF

FGF basic (FGF-2, HBGF-2) is one of at least 22 mitogenic proteins of the FGF family, which show 35-60% amino acid conservation (1-3). Unlike other FGFs, FGF acidic and basic lack signal peptides and are secreted by an alternate pathway. Storage pools within the cell or on cell surface heparan sulfate proteoglycans (HSPG) are likely. The predicted 18 kDa FGF basic isoform can be located in both the cytoplasm and the nucleus and is presumed to be the form secreted (4). Transcription from alternate start sites produces 21-23 kDa forms found only in the nucleus. High and low molecular weight human FGF basic targets the expression of different genes when expressed in NIH-3T3 cells (5). The 18 kDa rat sequence has 98% amino acid (aa) identity with mouse, and 96-97% aa identity with human, bovine and sheep FGF basic (6, 7). Autocrine, intracrine and paracrine actions of FGF basic have been identified. Binding of FGF to heparin or cell surface HSPG is necessary for binding, dimerization and activation of tyrosine kinase FGF receptors. FGF basic binds other proteins, polysaccharides and lipids with lower affinity (3). Expression of FGF basic is nearly ubiquitous but disruption of the mouse FGF basic gene gives a relatively mild phenotype, suggesting compensation by other FGF family members. FGF basic modulates such normal processes as angiogenesis, wound healing and tissue repair, embryonic development and differentiation, neuronal function and neural degeneration. Transgenic over-expression of FGF basic results in excessive proliferation and angiogenesis reminiscent of a variety of pathological conditions (1-3).

Item	Value
References	Coulier, F. et al. (1997) J. Mol. Evol. 44:43. Fernig, D. et al. (1994) Prog. Growth Factor Res. 5:353. Presta, M. et al. (2005) Cytokine Growth Factor Rev. 16:159. Claus, P. et al. (2003) J. Biol. Chem. 278:479. Quarto, N. et al. (2005) Gene 356:49. Tsuneto, M. et al. (2005) Biochem. Biophys. Res. Commun. 335:1239. Shimasaki, S. et al. (1988) Biochem. Biophys. Res. Commun. 157:256.
Long Name	Fibroblast Growth Factor basic
Entrez Gene IDs	2247 (Human); 14173 (Mouse); 281161 (Bovine); 403857 (Canine); 100033955 (Equine)
Alternate Names	Basic fibroblast growth factor, FGF basic, FGF-2, FGF2, FGFBprostatropin, HBGF-2, Prostatropin, bFGF, basic fibroblast growth factor bFGF, fibroblast growth factor 2 (basic), heparin-binding growth factor 2

Citations for Recombinant Rat FGF basic/FGF2/bFGF Protein (33)

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Citations are publications that use Bio-Techne products. Selected citations for Recombinant Rat FGF basic/FGF2/bFGF Protein include:

Species: Rat
Sample Types: Whole Cells
Applications: Bioassay

SY Park et al. (2022), Neuroprotective Effect of Bcl-2 on Lipopolysaccharide-Induced Neuroinflammation in Cortical Neural Stem Cells International Journal of Molecular Sciences, 23(12).
PMID: 35742844
M Furuhata et al. (2021), Real-time assessment of guided bone regeneration in critical size mandibular bone defects in rats using collagen membranes with adjunct fibroblast growth factor-2 Journal of dental sciences, 16(4):1170-1181.
PMID: 34484585
Species: Rat
Sample Types: Whole Cells
Applications: Bioassay

T Shimizu et al. (2021), Tension Sensor Based on Fluorescence Resonance Energy Transfer Reveals Fiber Diameter-Dependent Mechanical Factors During Myelination Frontiers in Cellular Neuroscience, 15(0):685044.
PMID: 34408628
Species: Rat
Sample Types: Whole Cells
Applications: Bioassay

X Wang et al. (2021), Photobiomodulation Promotes Repair Following Spinal Cord Injury by Regulating the Transformation of A1/A2 Reactive Astrocytes Frontiers in Neuroscience, 15(0):768262.
PMID: 34795557
Species: Rat
Sample Types: cell culture

M Nakamura et al. (2020), Canine mesenchymal stromal cell-conditioned medium promotes survival and neurite outgrowth of neural stem cells J. Vet. Med. Sci., 0(0).
PMID: 32249241
Species: Rat
Sample Types: Whole Cells
Applications: Bioassay

B Fan et al. (2020), Long noncoding RNA mediates stroke-induced neurogenesis Stem Cells, 0(0).
PMID: 32346940
Species: Rat
Sample Types: Whole Cells
Applications: Bioassay

S Mukherjee et al. (2020), Alcohol increases exosome release from microglia to promote complement C1q induced cellular death of proopiomelanocortin neurons in the hypothalamus in a rat model of fetal alcohol spectrum disorders J. Neurosci., 0(0).
PMID: 32887744
Species: Rat
Sample Types: Whole Cells
Applications: Bioassay

W Zhang et al. (2019), Implantation of engineered conduction tissue in the rat heart Mol Med Rep, 0(0).
PMID: 30720107
Species: Rat
Sample Types: Whole Cells
Applications: Bioassay

SR Sankavaram et al. (2019), Adult Neural Progenitor Cells Transplanted into Spinal Cord Injury Differentiate into Oligodendrocytes, Enhance Myelination, and Contribute to Recovery Stem Cell Reports, 0(0).
PMID: 31031190
Species: Rat
Sample Types: Whole Cells
Applications: Bioassay

EH Lee et al. (2019), Dopamine neuron induction and the neuroprotective effects of thyroid hormone derivatives Sci Rep, 9(1):13659.
PMID: 31541140
Species: Rat
Sample Types: Whole Cells
Applications: Bioassay

X Liang et al. (2019), Overexpression of cortistatin alleviates oxygen/glucose-deprivation-induced ER stress and prompts neural stem cell proliferation via SSTR2 Exp. Mol. Pathol., 0(0):104351.
PMID: 31809712
Species: Rat
Sample Types: Whole Cells
Applications: Bioassay

AE Zanno et al. (2019), Reducing Th2 inflammation through neutralizing IL-4 antibody rescues myelination in IUGR rat brain J Neurodev Disord, 11(1):34.
PMID: 31839002
Species: Rat
Sample Types: Whole Cells
Applications: Cell Culture

HH Park et al. (2019), Mitochondria damaged by Oxygen Glucose Deprivation can be Restored through Activation of the PI3K/Akt Pathway and Inhibition of Calcium Influx by Amlodipine Camsylate Sci Rep, 9(1):15717.
PMID: 31673096
Species: Rat
Sample Types: Whole Cells
Applications: Bioassay

X Sun et al. (2018), Differentiation of adipose-derived stem cells into Schwann cell-like cells through intermittent induction: potential advantage of cellular transient memory function Stem Cell Res Ther, 9(1):133.
PMID: 29751848
Species: Rat
Sample Types: Whole Cells
Applications: Bioassay

JD Karkera et al. (2017), Oncogenic Characterization and Pharmacologic Sensitivity of Activating Fibroblast Growth Factor Receptor (FGFR) Genetic Alterations to the Selective FGFR Inhibitor Erdafitinib Mol. Cancer Ther., 0(0).
PMID: 28416604
Species: Rat
Sample Types: Whole Cells
Applications: Bioassay

D Wang et al. (2017), Sox10(+) adult stem cells contribute to biomaterial encapsulation and microvascularization Sci Rep, 7(0):40295.
PMID: 28071739
Species: Rat
Sample Types: Whole Cells
Applications: Bioassay

JW Robinson et al. (2017), Dephosphorylation is the mechanism of fibroblast growth factor inhibition of guanylyl cyclase-B Cell. Signal., 40(0):222-229.
PMID: 28964968
Species: Rat
Sample Types: Whole Cells
Applications: Bioassay

Joong-Soo Han (2016), Hippocalcin Is Required for Astrocytic Differentiation through Activation of Stat3 in Hippocampal Neural Precursor Cells Front Mol Neurosci, 9(0):110.
PMID: 27840601
Species: Rat
Sample Types: In Vivo
Applications: In Vivo

(2016), Angiogenic microspheres promote neural regeneration and motor function recovery after spinal cord injury in rats Sci Rep, 6(0):33428.
PMID: 27641997
Species: Rat
Sample Types: Whole Cells
Applications: Bioassay

M Vitacolonn (2016), In-vivo quantification of the revascularization of a human acellular dermis seeded with EPCs and MSCs in co-culture with fibroblasts and pericytes in the dorsal chamber model in pre-irradiated tissue Cell Tissue Bank, 0(0).
PMID: 28004288
Species: Rat
Sample Types: Whole Cells
Applications: Bioassay

He X et al. (2015), Vitamin C facilitates dopamine neuron differentiation in fetal midbrain through TET1- and JMJD3-dependent epigenetic control manner. Stem Cells, 33(0):1320.
PMID: 25535150
Species: Rat
Sample Types: Whole Cells
Applications: Bioassay

Xu W et al. (2015), Perivascular-derived stem cells with neural crest characteristics are involved in tendon repair. Stem Cells Dev, 24(7):857-68.
PMID: 25381682
Species: Rat
Sample Types: Whole Cells
Applications: Bioassay

Romariz S et al. (2014), Long-lasting anxiolytic effect of neural precursor cells freshly prepared but not neurosphere-derived cell transplantation in newborn rats. BMC Neurosci, 15(0):94.
PMID: 25086450
Species: Rat
Sample Types: Whole Cells
Applications: Bioassay

Covacu R et al. (2014), Change of fate commitment in adult neural progenitor cells subjected to chronic inflammation. J Neurosci, 34(35):11571-82.
PMID: 25164655
Species: Rat
Sample Types: In Vivo
Applications: In Vivo

Reed et al. (2014), Pathogen sensing pathways in human embryonic stem cell derived-endothelial cells: role of NOD1 receptors. PLoS ONE, 9(4):e91119.
PMID: 24690886
Species: Rat
Sample Types: Whole Tissue
Applications: Bioassay

Dawes L et al. (2014), Interactions between lens epithelial and fiber cells reveal an intrinsic self-assembly mechanism. Dev Biol, 385(2):291-303.
PMID: 24211762
Species: Rat
Sample Types: Whole Cells
Applications: Bioassay

Kandasamy M et al. (2014), TGF-beta signalling in the adult neurogenic niche promotes stem cell quiescence as well as generation of new neurons. J Cell Mol Med, 18(7):1444-59.
PMID: 24779367
Species: Rat
Sample Types: Whole Cells
Applications: Bioassay

Matsushita et al. (2013), Meclozine facilitates proliferation and differentiation of chondrocytes by attenuating abnormally activated FGFR3 signaling in achondroplasia. PLoS ONE, 8(12):e81569.
PMID: 24324705
Species: Rat
Sample Types: Whole Cells
Applications: Bioassay

Qi et al. (2013), The effects of topographical patterns and sizes on neural stem cell behavior. PLoS ONE, 8(3):e59022.
PMID: 23527077
Species: Rat
Sample Types: Whole Tissue
Applications: Bioassay

Dawes L et al. (2013), Wnt-frizzled signaling is part of an FGF-induced cascade that promotes lens fiber differentiation. Invest Ophthalmol Vis Sci, 54(3):1582-90.
PMID: 23385791
Species: Rat
Sample Types: Whole Cells
Applications: Bioassay

Asumda FZ et al. (2011), Age-related changes in rat bone-marrow mesenchymal stem cell plasticity. BMC Cell Biol., 12(0):44.
PMID: 21992089
Species: Mouse
Sample Types: Whole Tissue
Applications: Bioassay

Krejci P et al. (2009), Fibroblast growth factor inhibits interferon gamma-STAT1 and interleukin 6-STAT3 signaling in chondrocytes. Cell. Signal., 21(1):151-60.
PMID: 18950705
Species: Rat
Sample Types: Whole Cells
Applications: Bioassay

Hunt DP et al. (2007), A highly enriched niche of precursor cells with neuronal and glial potential within the hair follicle dermal papilla of adult skin. Stem Cells, 26(1):163-72.
PMID: 17901404

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FAQs for Recombinant Rat FGF basic/FGF2/bFGF Protein

What receptors does FGF basic bind?

FGF receptor specificity has been reviewed in multiple citations. Please find more information at: //www.bio-techne.com/resources/articles/fibroblast-growth-factors-and-their-receptors

Recombinant Rat FGF basic/FGF2/bFGF Protein

R&D Systems, Inc. a Bio-Techne Brand

Key Product Details

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Product Specifications for Recombinant Rat FGF basic/FGF2/bFGF Protein

Formulation, Preparation and Storage

Carrier Free

3339-FB

Carrier

3339-FB/CF

Carrier Free

Reconstitution Calculator

Background: FGF basic/FGF2/bFGF

Citations for Recombinant Rat FGF basic/FGF2/bFGF Protein (33)

FAQs for Recombinant Rat FGF basic/FGF2/bFGF Protein

Product Documents for Recombinant Rat FGF basic/FGF2/bFGF Protein

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