Skip to main content

Recombinant Rat Fas/TNFRSF6/CD95 Fc Chimera Protein, CF

Catalog # 2159-FA | R&D Systems, Inc. a Bio-Techne Brand
Catalog #
Availability
Size / Price
Qty
Loading...
2159-FA-050

Key Product Details

Source

NS0

Accession #

Structure / Form

Disulfide-linked homodimer

Conjugate

Unconjugated

Applications

Bioactivity

Product Specifications

Source

Mouse myeloma cell line, NS0-derived rat Fas/TNFRSF6/CD95 protein
Rat Fas
(Gln22 - Lys170)
Accession # NP_631933
IEGRMD Human IgG1
(Pro100 - Lys330)
N-terminus C-terminus

Purity

>90%, by SDS-PAGE under reducing conditions and visualized by silver stain.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

No results obtained: Gln22 predicted

Predicted Molecular Mass

43.3 kDa (monomer)

SDS-PAGE

60-65 kDa, reducing conditions

Activity

Measured by its ability to inhibit Fas Ligand-induced apoptosis of Jurkat human acute T cell leukemia cells. Cheng, J. et al. (1994) Science 263:1759.
The ED50 for this effect is 0.05-0.25 µg/mL in the presence of 100 ng/mL recombinant rat Fas Ligand.

Formulation, Preparation and Storage

2159-FA
Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution
Reconstitute at 100 μg/mL in sterile PBS.

Reconstitution Buffer Available:
Size / Price
Qty
Loading...
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Fas/TNFRSF6/CD95

Fas, also known as APO-1 or CD95, belongs to the death receptor subfamily of the TNF receptor superfamily and is designated TNFRSF6 (1 ‑ 3). Rat Fas cDNA encodes 324 amino acids (aa) that include a 21 aa signal peptide, a 150 aa extracellular domain (ECD) that contains three cysteine-rich TNFR repeats, a 17 aa transmembrane sequence, and a 136 aa cytoplasmic domain containing a death domain (DD), which is required for transducing apoptotic signals (4). Mature rat Fas ECD shares 68% aa sequence identity with mouse Fas, and 56 ‑ 58% with human, feline, bovine and porcine Fas. A 114 aa potential rat Fas isoform contains an alternate start site at aa 211, thus lacking all three TNFR repeats (5). The Fas ligand (FasL, TNFSF6) is a type II transmembrane protein of the TNF family that can be expressed on activated T-lymphocytes, NK cells and cells in immune privileged sites, or shed in soluble form (2). Engagement of FAS induces oligomerization of preformed Fas trimers (1, 2). The activated receptor recruits the adaptor molecule FADD to form the Death-Inducing Signaling Complex (DISC). Upon activation, caspases in the DISC initiate the apoptotic signaling cascade (6). Fas is prominent in epithelial cells, hepatocytes, activated mature lymphocytes, virus-transformed lymphocytes and tumor cells. It is an essential mediator in the activation-induced death of T lymphocytes that terminates the immune reaction (1, 2, 7). In immune-privileged tissues, infiltrating Fas-bearing lymphocytes and inflammatory cells are killed by FasL engagement (8). Both humans and mice with genetic defects in Fas accumulate abnormal lymphocytes and develop systemic autoimmunity (1 ‑ 3). The Fas pathway also appears to cross-communicate with the BIM (mitochondrial/intrinsic) apoptosis pathway (1).

References

  1. Bouillet, P. and L.A. O’Reilly (2009) Nat. Rev. Immunol. 9:514.
  2. Strasser, A. et al. (2009) Immunity 30:180.
  3. Ashkenazi, A. and V. Dixit (1999) Curr. Opin. Cell Biol. 11:255.
  4. SwissProt accession Q63199.
  5. GenBank protein accession EDM13159.
  6. Thorburn, A. (2003) Cellular Signaling 16:139.
  7. Barreiro, R. et al. (2004) J. Immunol. 173:1519.
  8. Ferguson, T.A. and T.S Griffith (2006) Immunol. Rev. 213:228.

Long Name

Fibroblast-associated

Alternate Names

Apo-1, APT1, CD95, TNFRSF6

Entrez Gene IDs

355 (Human); 14102 (Mouse); 246097 (Rat); 102140989 (Cynomolgus Monkey); 493881 (Feline)

Gene Symbol

FAS

UniProt

Product Documents for Recombinant Rat Fas/TNFRSF6/CD95 Fc Chimera Protein, CF

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Note: Certificate of Analysis not available for kit components.

Product Specific Notices for Recombinant Rat Fas/TNFRSF6/CD95 Fc Chimera Protein, CF

For research use only

Citations for Recombinant Rat Fas/TNFRSF6/CD95 Fc Chimera Protein, CF (3)

Showing  1 - 3 of 3 publications Showing All
Filter By:
Search Title:

Citations are publications that use Bio-Techne products. Selected citations for Recombinant Rat Fas/TNFRSF6/CD95 Fc Chimera Protein, CF include:

There are no citations that match your criteria.

Showing  1 - 3 of 3 publications Showing All

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

÷
=
Loading...
Setting the standard in quality research reagents