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Recombinant Rat EphB1 Fc Chimera Protein, CF

Bio-Techne includes R&D Systems | Catalog # 1596-B1

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1596-B1-200

Key Product Details

Source

NS0

Accession #

Structure / Form

Disulfide-linked homodimer

Conjugate

Unconjugated

Applications

Binding Activity

Product Specifications

Source

Mouse myeloma cell line, NS0-derived rat EphB1 protein
Rat EphB1
(Met18-Gln538)
Accession # P09759
IEGRMD Human IgG1
(Pro100-Lys330)
N-terminus C-terminus

Purity

>95%, by SDS-PAGE under reducing conditions and visualized by silver stain.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

Met18

Predicted Molecular Mass

85 kDa (monomer)

SDS-PAGE

102 kDa, reducing conditions

Activity

Measured by its binding ability in a functional ELISA.
Immobilized Recombinant Rat EphB1 Fc Chimera at 2 µg/mL (100 µL/well) can bind recombinant mouse Ephrin‑B1 Fc Chimera with a linear range of 0.31-20 ng/mL.
Optimal dilutions should be determined by each laboratory for each application.

Formulation, Preparation and Storage

1596-B1
Formulation Lyophilized from a 0.2 μm filtered solution in Tris.
Reconstitution
Reconstitute at 200 μg/mL in sterile PBS.

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Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: EphB1

EphB1, also known as Elk, Cek6, Net, and Hek6 (1), is a member of the Eph receptor family which binds members of the ephrin ligand family. There are two classes of receptors, designated A and B. Both the A and B class receptors have an extracellular region consisting of a globular domain, a cysteine-rich domain, and two fibronectin type III domains. This is followed by the transmembrane region and the cytoplasmic region. The cytoplasmic region contains a juxtamembrane motif with two tyrosine residues, which are the major autophosphorylation sites, a kinase domain, and a conserved sterile alpha motif (SAM) in the carboxy tail which contains one conserved tyrosine residue. Activation of kinase activity occurs after ligand recognition and binding. EphB1 has been shown to bind ephrin-B2, ephrin-B1,
ephrin‑A3, ephrin-A1, ephrin-A4, and ephrin-B3 (2, 3). The extracellular domains of human and rat EphB1 share 99% amino acid identity. Only membrane-bound or
Fc‑clustered ligands are capable of activating the receptor in vitro. While soluble monomeric ligands bind the receptor, they do not induce receptor autophosphorylation and activation (2). In vivo, the ligands and receptors display reciprocal expression (3). It has been found that nearly all the receptors and ligands are expressed in developing and adult neural tissue (3). The ephrin/Eph families also appear to play a role in angiogenesis (3).

References

  1. Eph Nomenclature Committee [letter]. (1997) Cell 90:403.
  2. Flanagan, J.G. and P. Vanderhaeghen (1998) Annu. Rev. Neurosci. 21:309.
  3. Pasquale, E.B. (1997) Curr. Opin. Cell Biol. 9:608.

Long Name

Eph Receptor B1

Alternate Names

Cek6, Elk, Hek6, Net

Entrez Gene IDs

2047 (Human); 270190 (Mouse); 24338 (Rat)

Gene Symbol

EPHB1

UniProt

Additional EphB1 Products

Product Documents for Recombinant Rat EphB1 Fc Chimera Protein, CF

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Note: Certificate of Analysis not available for kit components.

Product Specific Notices for Recombinant Rat EphB1 Fc Chimera Protein, CF

For research use only

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