Recombinant Rat EGF Protein

Name: Recombinant Rat EGF Protein
Brand: R&D Systems
SKU: 3214-EG

R&D Systems, Inc. a Bio-Techne Brand

Catalog # 3214-EG

Citations (12)

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Setting the standard in quality research reagents

Key Product Details

Source:	E. coli
Accession #:	P07522
Applications:	Bioactivity

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Product Specifications for Recombinant Rat EGF Protein

Source	E. coli-derived rat EGF protein Asn974-Arg1026, with an N-terminal Met
Purity	>97%, by SDS-PAGE under reducing conditions and visualized by silver stain.
Endotoxin level	<0.10 EU per 1 μg of the protein by the LAL method.
N-terminal sequence Analysis	Met
Predicted Molecular Mass	6.3 kDa
Activity	Measured in a cell proliferation assay using Balb/3T3 mouse embryonic fibroblast cells. Rubin, J.S. et al. (1991) Proc. Natl. Acad. Sci. USA 88:415. The ED₅₀ for this effect is 10-60 pg/mL.

Formulation, Preparation and Storage

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

3214-EG

Carrier

Formulation	Lyophilized from a 0.2 μm filtered solution in Acetonitrile and TFA with BSA as a carrier protein.
Reconstitution	Reconstitute at 100 μg/mL in sterile 10 mM Acetic Acid containing at least 0.1% human or bovine serum albumin.
Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage:	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 3 months, -20 to -70 °C under sterile conditions after reconstitution.

3214-EG/CF

Carrier Free

Formulation	Lyophilized from a 0.2 μm filtered solution in Acetonitrile and TFA.
Reconstitution	Reconstitute at 100 μg/mL in sterile 10 mM Acetic Acid.
Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage:	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: EGF

Epidermal growth factor (EGF) is a small, potent growth factor capable of inducing cell proliferation, differentiation, and survival. EGF is the founding member of the EGF family that also includes TGF-alpha, amphiregulin (AR), betacellulin (BTC), epiregulin (EPR), heparin‑binding EGF‑like growth factor (HB‑EGF), epigen, and the neuregulins (NRG)-1 through -6 (1). Members of The EGF family are characterized by a shared structural motif, the EGF‑like domain, which contains three intramolecular disulfide bonds that are formed by six similarly spaced, conserved cysteine residues (2). These disulfide bonds are essential for proper protein conformation and receptor binding. All EGF family members are synthesized as type I transmembrane precursor proteins that may contain several EGF domains in the extracellular region. The mature proteins are released from the cell surface by regulated proteolysis (1). The full length EGF protein is 1207 amino acids (aa) (EGF precursor) containing nine EGF domains and nine LDLR class B repeats. However, the mature protein is much smaller, only 53 aa, and is generated by proteolytic cleavage of the EGF domain proximal to the transmembrane region (3). EGF is well conserved across mammals with mature human EGF 70% identical to mature mouse and rat EGF. Physiologically, EGF is found in various body fluids, including blood, milk, urine, saliva, seminal fluid, pancreatic juice, cerebrospinal fluid, and amniotic fluid (4). EGF is a high affinity ligand of the EGF receptor (ErbB). Four ErbB (HER) family receptor tyrosine kinases including EGFR/ErbB1, ErbB2, ErbB3 and ErbB4, mediate responses to EGF family members (5). EGF binding induces dimerization of the EGF receptor resulting in activation of the protein tyrosine kinase signaling pathway. These receptors undergo a complex pattern of ligand-induced homo- or hetero-dimerization to transduce EGF family signals (6, 7). EGF binds ErbB1 and depending on the context, induces the formation of homodimers or heterodimers containing ErbB2. Dimerization results in autophosphorylation of the receptor at specific tyrosine residues to create docking sites for a variety of signaling molecules (5, 8). Biological activities ascribed to EGF include epithelial development, angiogenesis, inhibition of gastric acid secretion, fibroblast proliferation, and colony formation of epidermal cells in culture.

Item	Value
References	Harris, R.C. et al. (2003) Exp. Cell Res. 284:2. Carpenter, G. and Cohen, S. (1990) J. Biol. Chem. 265:7709. Saggi, S.J. et al. (1992) DNA Cell Biol. 11:481. Carpenter, G. and Zendegui, J.G. (1986) Exp. Cell Res. 164:1. Jorissen, R.N. et al. (2003) Exp. Cell Res. 284:31. Gamett, D.C. et al. (1997) J. Biol. Chem. 272:12052. Qian, X. et al. (1994) Proc. Natl. Acad. Sci. 91:1500. Qian, X. et al. (1999) J. Biol. Chem. 274:574.
Long Name	Epidermal Growth Factor
Entrez Gene IDs	1950 (Human); 13645 (Mouse); 25313 (Rat)
Alternate Names	EGF, HOMG4, URG, Urogastrone, beta-urogastrone, epidermal growth factor, epidermal growth factor (beta-urogastrone), hEGF, pro-epidermal growth factor

Citations for Recombinant Rat EGF Protein (12)

Showing 1 - 10 of 12 citations Showing All

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Citations are publications that use Bio-Techne products. Selected citations for Recombinant Rat EGF Protein include:

Species: Rat
Sample Types: Whole Cells
Applications: Bioassay

A López-Márq et al. (2022), Sox9 is involved in the thyroid differentiation program and is regulated by crosstalk between TSH, TGFbeta and thyroid transcription factors Scientific Reports, 12(1):2144.
PMID: 35140269
Species: Rat
Sample Types: Whole Cell
Applications: Cell Culture

Z Berkova et al. (2022), Decellularized Pancreatic Tail as Matrix for Pancreatic Islet Transplantation into the Greater Omentum in Rats Journal of functional biomaterials, 13(4).
PMID: 36278640
Species: Rat
Sample Types: Whole Cells
Applications: Bioassay

LT Frasson et al. (2021), Let-7, Lin28 and Hmga2 Expression in Ciliary Epithelium and Retinal Progenitor Cells Investigative Ophthalmology Visual Science, 2021;62(3):31.
PMID: 33749722
Species: Rat
Sample Types: Cells
Applications: Cell Culture

M Nakamura et al. (2020), Canine mesenchymal stromal cell-conditioned medium promotes survival and neurite outgrowth of neural stem cells J. Vet. Med. Sci., 0(0).
PMID: 32249241
Species: Rat
Sample Types: Whole Cells
Applications: Bioassay

B Fan et al. (2020), Long noncoding RNA mediates stroke-induced neurogenesis Stem Cells, 0(0).
PMID: 32346940
Species: Rat
Sample Types: Whole Cells
Applications: Bioassay

W Zhang et al. (2019), Implantation of engineered conduction tissue in the rat heart Mol Med Rep, 0(0).
PMID: 30720107
Species: Rat
Sample Types: Whole Cells
Applications: Bioassay

D Stöckmann et al. (2018), Non-Canonical Activation of the Epidermal Growth Factor Receptor by Carbon Nanoparticles Nanomaterials (Basel), 8(4).
PMID: 29690640
Species: Rat
Sample Types: Tissue Homogenates
Applications: Bioassay

M Zhang et al. (2017), Comparative Analysis of the Cell Fates of Induced Schwann Cells from Subcutaneous Fat Tissue and Naï¿½ve Schwann Cells in the Sciatic Nerve Injury Model Biomed Res Int, 2017(0):1252851.
PMID: 28713821
Species: Rat
Sample Types: In Vivo
Applications: In Vivo

O Stojadinov et al. (2017), Wound healing protects against chemotherapy-induced alopecia in young rats via up-regulating interleukin-1?-mediated signaling Heliyon, 3(5):e00309.
PMID: 28607955
Matsumoto et al. (2014), A combination of Wnt and growth factor signaling induces Arl4c expression to form epithelial tubular structures. EMBO J, 33(7):702-18.
PMID: 24562386
Species: Rat
Sample Types: Whole Cells
Applications: Bioassay

Kandasamy M et al. (2014), TGF-beta signalling in the adult neurogenic niche promotes stem cell quiescence as well as generation of new neurons. J Cell Mol Med, 18(7):1444-59.
PMID: 24779367
Species: Rat
Sample Types: Whole Cells
Applications: Bioassay

Gilbert RE et al. (2011), Histone deacetylase inhibition attenuates diabetes-associated kidney growth: potential role for epigenetic modification of the epidermal growth factor receptor. Kidney Int., 79(12):1312-21.
PMID: 21389970

Recombinant Rat EGF Protein

R&D Systems, Inc. a Bio-Techne Brand

Key Product Details

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Product Specifications for Recombinant Rat EGF Protein

Formulation, Preparation and Storage

Carrier Free

3214-EG

Carrier

3214-EG/CF

Carrier Free

Reconstitution Calculator

Background: EGF

Citations for Recombinant Rat EGF Protein (12)

Product Documents for Recombinant Rat EGF Protein

Product Datasheets

COA

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SDS

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