Recombinant Rat DLL1 Fc Chimera Protein, CF

Name: Recombinant Rat DLL1 Fc Chimera Protein, CF
Brand: R&D Systems
SKU: 3970-DL
Rating: 5 (1 reviews)

R&D Systems, Inc. a Bio-Techne Brand

Catalog # 3970-DL

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Key Product Details

Source:	CHO
Accession #:	P97677
Structure / Form:	Disulfide-linked homodimer
Applications:	Binding Activity

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Product Specifications for Recombinant Rat DLL1 Fc Chimera Protein, CF

Source

Chinese Hamster Ovary cell line, CHO-derived rat DLL1 protein

Rat DLL-1 (Ser22 - Ser534) Accession # P97677	IEGRMDP	Mouse IgG_2A (Glu98 - Lys330)
N-terminus		C-terminus

Purity

>95%, by SDS-PAGE under reducing conditions and visualized by silver stain

Endotoxin level

<0.10 EU per 1 μg of the protein by the LAL method.

N-terminal sequence Analysis

Ser22

Predicted Molecular Mass

82.6 kDa (monomer)

SDS-PAGE

90-95 kDa, reducing conditions

Activity

Measured by its binding ability in a functional ELISA. When rrNotch-1 (Catalog # 1057-TK) is coated at 5 μg/mL, rrDLL-1 binds with an apparent K_D<5 nM.

Formulation, Preparation and Storage

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

3970-DL

Formulation	Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution	Reconstitute at 200 μg/mL in PBS. Reconstitution Buffer Available: Size / Price Qty Loading... RB01:
Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage:	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: DLL1

Delta-like protein 1 (DLL1) is a 90 ‑ 100 kDa type I transmembrane protein in the Delta/Serrate/Lag-2 (DSL) family of Notch ligands. Mature rat DLL1 consists of a 520 amino acid (aa) extracellular domain (ECD) with one DSL domain and eight EGF‑like repeats, a 23 aa transmembrane segment, and a 154 aa cytoplasmic domain (1). Within the ECD, rat DLL1 shares 90% and 95% aa sequence identity with human and mouse DLL1, respectively. It shares 26%, 36%, and 53% aa sequence identity with rat DLL2, 3, and 4, respectively. The ADAM9, 12, or 17‑ mediated proteolysis of DLL1 releases a 60 kDa ECD fragment and regulates the Notch-dependent proliferation of hematopoietic and myogenic progenitor cells (2 ‑ 4). The residual membrane-bound portion of DLL1 can be cleaved by presenilin-dependent gamma -secretase, enabling the cytoplasmic domain to migrate to the nucleus (5). DLL1 localizes to adherens junctions on neuronal processes through its association with the scaffolding protein MAGI1 (6). DLL1 is widely expressed, and it plays an important role in embryonic somite formation, cochlear hair cell differentiation, lymphocyte differentiation, and the maintenance of neural and myogenic progenitor cells (4, 7 ‑ 13). The up‑regulation of DLL1 in arterial endothelial cells following injury or angiogenic stimulation is central to postnatal arteriogenesis (14). DLL1 is also overexpressed in cervical carcinoma and glioma and contributes to tumor progression (15 ‑ 16).

Item	Value
References	Bettenhausen, B. et al. (1995) Development 121:2407. Dyczynska, E. et al. (2007) J. Biol. Chem. 282:436. Karanu, F.N. et al. (2001) Blood 97:1960. Sun, D. et al. (2008) J. Cell Sci. 121:3815. Ikeuchi, T. and S.S. Sisodia (2003) J. Biol. Chem. 278:7751. Mizuhara, E. et al. (2005) J. Biol. Chem. 280:26499. Takahashi, Y. et al. (2003) Development 130:4259. Teppner, I. et al. (2007) BMC Dev. Biol. 7:68. Kiernan, A.E. et al. (2005) Development 132:4353. Schmitt, T.M. and J.C. Zuniga-Pflucker (2002) Immunity 17:749. Hozumi, K. et al. (2004) Nat. Immunol. 5:638. Shimojo, H. et al. (2008) Neuron 58:52. Schuster-Gossler, K. et al. (2007) Proc. Natl. Acad. Sci. 104:537. Limbourg, A. et al. (2007) Circ. Res. 100:363. Purow, B.W. et al. (2005) Cancer Res. 65:2353. Gray, G.E. et al. (1999) Am. J. Pathol. 154:785.
Long Name	Delta-like 1
Entrez Gene IDs	28514 (Human); 13388 (Mouse); 84010 (Rat)
Alternate Names	DL1, DLL1, Delta, Delta 1, Delta1, Drosophila Delta homolog 1, H-Delta-1, delta (Drosophila)-like 1, delta-like 1 (Drosophila), delta-like protein 1

Citations for Recombinant Rat DLL1 Fc Chimera Protein, CF (1)

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Citations are publications that use Bio-Techne products. Selected citations for Recombinant Rat DLL1 Fc Chimera Protein, CF include:

Species: Mouse
Sample Types: Whole Cells
Applications: Bioassay

(2016), Delta/Notch-Like EGF-Related Receptor (DNER) Is Not a Notch Ligand PLoS ONE, 11(9):e0161157.
PMID: 27622512

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Recombinant Rat DLL1 Fc Chimera Protein, CF

Name: Anonymous

Application: In vitro bioactivity in cell culture

Verified Customer | Posted 08/25/2021

fibroblast derived ipsc lines were grown for 48 hr on gelatine-coated plates which contain 100 ng/ml rDLL1. mRNA level of Hes1 was detected to check if DLL1 stimulated Hes1 expression.

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Recombinant Rat DLL1 Fc Chimera Protein, CF

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Product Specifications for Recombinant Rat DLL1 Fc Chimera Protein, CF

Formulation, Preparation and Storage

Carrier Free

Reconstitution Calculator

Background: DLL1

Citations for Recombinant Rat DLL1 Fc Chimera Protein, CF (1)

Customer Reviews for Recombinant Rat DLL1 Fc Chimera Protein, CF (1)

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Recombinant Rat DLL1 Fc Chimera Protein, CF

Product Documents for Recombinant Rat DLL1 Fc Chimera Protein, CF

Product Datasheets

COA

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SDS

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