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Recombinant Rat CD96 Fc Chimera Protein, CF

Catalog # 10400-CD | R&D Systems, Inc. a Bio-Techne Brand
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10400-CD-050

Key Product Details

Source

NS0

Accession #

Structure / Form

Disulfide-linked homodimer

Conjugate

Unconjugated

Applications

Bioactivity

Product Specifications

Source

Mouse myeloma cell line, NS0-derived rat CD96 protein
Rat CD96
(Glu25-Met537)
Accession # Q5BK49.1
IEGRMDP Mouse IgG2a
(Glu98-Lys330)
N-terminus C-terminus

Purity

>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

Glu25

Predicted Molecular Mass

84 kDa

SDS-PAGE

135-160 kDa, under reducing conditions

Activity

Measured by its binding ability in a functional ELISA.
When Recombinant Rat CD155/PVR (Catalog # 9764-CD) is immobilized at 1 µg/mL (100 µL/well), Recombinant Rat CD96 Fc Chimera (Catalog # 10400-CD) binds with an ED50 of 10-70 ng/mL

Scientific Data Images for Recombinant Rat CD96 Fc Chimera Protein, CF

Recombinant Rat CD96 Fc Chimera Protein Binding Activity

Recombinant Rat CD96 Fc Chimera Protein Binding Activity

When Recombinant Rat CD155/PVR (Catalog # 9764-CD) is immobilized at 1 µg/mL (100 µL/well), Recombinant Rat CD96 Fc Chimera (Catalog # 10400-CD) binds with an ED50 of 10-70 ng/mL.
Recombinant Rat CD96 Fc Chimera Protein SDS-PAGE

Recombinant Rat CD96 Fc Chimera Protein SDS-PAGE

2 μg/lane of Recombinant Rat CD96 Fc Chimera (Catalog # 10400-CD) was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by Coomassie® Blue staining, showing bands at 135-160 kDa and 270-320 kDa, respectively.

Formulation, Preparation and Storage

10400-CD
Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution Reconstitute at 500 μg/mL in PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: CD96

CD96, also known as Tactile (T cell-activated increased late expression), is a 160 kDa type I transmembrane glycoprotein of the Ig superfamily that shows peak expression 6-9 days after activation of T, NK, and a subpopulation of B cells (1, 2). The mature extracellular domain (ECD) of rat CD96 contains three highly N- and O-glycosylated Ig-like domains, with the two N-terminal domains being V-type and the distal domain a C-type structure (1). Within the mature ECD, rat CD96 shares 79% and 52% amino acid sequence identity with mouse and human CD96, respectively. In human, a splice variant with a 16 aa deletion in the second V-type domain, called CD96v2, can be expressed (2). CD96 is also frequently expressed on acute myeloid leukemia and myelodysplastic stem cells (3, 4). It is expressed on CD4+ and CD8+ T cells, plus NK cells and select B cells (5). Low expression of adhesive human CD96 is a rare cause of C syndrome, a set of developmental anomalies in cranial bone (trigonocephaly), skin and viscera, demonstrating a role for CD96 in development of these tissues (2, 6). An 80 kDa soluble form is elevated in human serum during chronic hepatitis B (9). The most N-terminal Ig-like domain of human CD96 binds to CD155/PVR (poliovirus receptor), but CD96/CD155 interaction is species-specific (2, 7, 10). On NK cells, co-stimulatory CD96 and DNAM-1 (CD226) are thought to have paired activity with inhibitory TIGIT, all of which bind CD155 and various nectins (11, 12). CD96 can promote NK cell adhesion to, and killing of target cells, including tumors that express CD155 (10, 11).

References

  1. Wang, P.L. et al. (1992) J. Immunol. 148:2600.
  2. Meyer, D. et al. (2009) J. Biol. Chem. 284:2235.
  3. Hosen, N. et al. (2007) Proc. Natl. Acad. Sci. USA 104:11008.
  4. Xie, W. et al. (2010) Cytometry A 77:840.
  5. Eriksson, E. M. et al. (2012) PLOS One 7:e51696.
  6. Kaname, T. et al. (2007) Am. J. Hum. Genet. 81:835.
  7. Seth, S. et al. (2007) Biochem. Biophys. Res. Commun. 364:959.
  8. Protein Accession # BAE32358.
  9. Gong, J. et al. (2008) Clin. Exp. Immunol. 155:207.
  10. Fuchs, A. et al. (2004) J. Immunol. 172:3394.
  11. Stanietsky, N. and O. Mandelboim (2010) FEBS Lett. 584:4895.
  12. Xu, Z. and B. Jin (2010) Cell. Mol. Immunol. 7:11.

Alternate Names

CD96, TACTILE

Entrez Gene IDs

10225 (Human); 84544 (Mouse); 498079 (Rat); 102121080 (Cynomolgus Monkey)

Gene Symbol

CD96

UniProt

Additional CD96 Products

Product Documents for Recombinant Rat CD96 Fc Chimera Protein, CF

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Note: Certificate of Analysis not available for kit components.

Product Specific Notices for Recombinant Rat CD96 Fc Chimera Protein, CF

For research use only

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