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Recombinant Rat CD19 Fc Chimera Protein, CF

Catalog # 10525-CD | R&D Systems, Inc. a Bio-Techne Brand
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10525-CD-050

Key Product Details

Source

CHO

Accession #

Structure / Form

Disulfide-linked homodimer

Conjugate

Unconjugated

Applications

Bioactivity

Product Specifications

Source

Chinese Hamster Ovary cell line, CHO-derived rat CD19 protein
Rat CD19
(Arg19-Gly287)
Accession # NP_001013255.2
IEGRMDP Mouse IgG2a
(Glu98-Lys330)
N-terminus C-terminus

Purity

>90%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

Arg19

Predicted Molecular Mass

57 kDa

SDS-PAGE

80-95 kDa, under reducing conditions

Activity

Measured by its binding ability in a functional ELISA.
When Recombinant Rat CD19 Fc Chimera(Catalog # 10525-CD) is immobilized at 5 µg/mL,100 µL/well, recombinant biotinylated human CD81 Fc Chimera binds with an ED50 of 10-60 μg/mL.

Formulation, Preparation and Storage

10525-CD
Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution Reconstitute at 500 μg/mL in PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: CD19

CD19, also known as B4, is a transmembrane glycoprotein of the immunoglobulin superfamily that plays a central role in B cell activation and humoral immune responses (1, 2). CD19 consists of an extracellular domain (ECD) with two C2-type Ig-like domains, a transmembrane segment, and a cytoplasmic domain with nine tyrosine residues, 3 of which are critical for function (1,2). Within the mature ECD, rat CD19 shares 57% and 88% amino acid sequence identity with human and mouse CD19, respectively. CD19 is expressed throughout B cell development from pre‑B cells through mature B cells, and it is commonly used as a B cell lineage marker (1, 2). It is required for the responsiveness of mature B cell to antigen stimulation, germinal center development, and antibody affinity maturation (1, 2). CD19 associates with the B cell antigen receptor (BCR), CD81, CD38, CD21, CD22, and IFITM1/CD225/Leu-13 (1, 3). These associations enable CD19 to amplify B cell signaling and reduce the threshold for antigen stimulation through the BCR (1, 3). CD19 polymorphisms and up-regulation can lead to the development of autoimmunity by promoting autoantibody production (2). CD19+ target cells can be recognized and killed by T cells that express anti-CD19 chimeric antigen receptors (CARs) (4). This interaction has been shown to be beneficial in the treatment of various B-cell malignancies, such as leukemias and lymphomas (4, 5).

References

  1. Wang, K. et al. (2012) Exp. Hematol Oncol. 1:36.
  2. Del Nargo, C.J. et al. (2005) Immunol Res. 31:229.
  3. Yu, F. et al. (2010) J Neurooncol. 103:187.
  4. Kochenderfer, J. et al. (2015) J Clin Oncol. 33:540.
  5. Lee, D. et al. (2015) Lancet. 385:517.

Alternate Names

CD19, CVID3, Leu-12

Entrez Gene IDs

930 (Human); 12478 (Mouse); 365367 (Rat); 397669 (Porcine); 607898 (Canine); 102145514 (Cynomolgus Monkey); 100147468 (Equine)

Gene Symbol

CD19

UniProt

Product Documents for Recombinant Rat CD19 Fc Chimera Protein, CF

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Note: Certificate of Analysis not available for kit components.

Product Specific Notices for Recombinant Rat CD19 Fc Chimera Protein, CF

For research use only

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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