Recombinant Rat CCL2/JE/MCP-1 Protein

Name: Recombinant Rat CCL2/JE/MCP-1 Protein
Brand: R&D Systems
SKU: 3144-JE
Rating: 5 (1 reviews)

R&D Systems, Inc. a Bio-Techne Brand

Catalog # 3144-JE

Citations (7)

(1)

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Key Product Details

Source:	NS0
Accession #:	P14844
Applications:	Bioactivity

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Product Specifications for Recombinant Rat CCL2/JE/MCP-1 Protein

Source	Mouse myeloma cell line, NS0-derived rat CCL2/JE/MCP-1 protein Gln24-Asn148
Purity	>90%, by SDS-PAGE under reducing conditions and visualized by silver stain.
Endotoxin level	<0.10 EU per 1 μg of the protein by the LAL method.
N-terminal sequence Analysis	No results obtained: Gln24 predicted
Predicted Molecular Mass	14 kDa
SDS-PAGE	25-35 kDa, reducing conditions
Activity	Measured by its ability to chemoattract BaF3 mouse pro‑B cells transfected with human CCR2A. The ED₅₀ for this effect is 30-150 ng/mL.

Formulation, Preparation and Storage

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

3144-JE

Carrier

Formulation	Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose and with BSA as a carrier protein.
Reconstitution	Reconstitute at 100 μg/mL in sterile PBS containing at least 0.1% human or bovine serum albumin.
Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage:	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 3 months, -20 to -70 °C under sterile conditions after reconstitution.

3144-JE/CF

Carrier Free

Formulation	Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.
Reconstitution	Reconstitute at 100 μg/mL in sterile PBS.
Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage:	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 3 months, -20 to -70 °C under sterile conditions after reconstitution.

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The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: CCL2/JE/MCP-1

Rat CCL2 is a member of the beta (C-C) subfamily of chemokines that is a chemoattractant for monocytes and basophils but not eosinophils or neutrophils (1-4). Rat CCL2 is secreted as a 14 kDa glycoprotein monomer (5) but noncovalent dimers probably occur (1). The first five amino acids of the mature protein are essential for activity; deletion of the N-terminal glutamine, which is pyrrolidone carboxylic acid-modified, dramatically decreases activity on basophils and, surprisingly, stimulates eosinophil chemotaxis (4). The rat CCL2 propeptide shares 82% amino acid (a.a.) identity with mouse CCL2 over the 148 a.a. sequence and 57%, 52%, 52%, 52% and 52% a.a. identity with equine, human, porcine, canine and guinea pig CCL2, respectively, over the first 100 aa. Rat and mouse CCL2 have a 49 aa extension at the C-terminus as compared to human CCL2. Fibroblasts, tumor cells, smooth muscle cells, endothelial cells, and mononuclear phagocytes can produce CCL2 either constitutively or upon mitogenic stimulation. CCL2 is best known as a chemotactic agent for mononuclear cells. It also induces enzyme and cytokine release by monocytes, NK cells and lymphocytes, and histamine release by basophils, primarily due to interaction with CCR2 receptors on these cells. Additionally, it is believed to reduce IL-12 production by dendritic cells and promote a Th2 phenotype in CD4⁺ T cells. The role of CCL2 in recruiting monocytes to sites of inflammation is implicated in the pathology of multiple sclerosis, atherosclerosis and allergic asthma (6).

Item	Value
References	VanCoillie, E.V. et al. (1999) Cytokine Growth Factor Rev. 10:61. Luther, S.A. and J.G. Cyster (2001) Nat. Immunol. 2:102. Lu, B. et al. (1998) J. Exp. Med. 187:601. Weber, M. et al. (1996) J. Exp. Med. 183:681. Yoshimura, T. et al. (1991) Biochem. Biophys. Res. Commun. 174:504. Daly, C. et al. (2003) Microcirculation 10:247.
Entrez Gene IDs	6347 (Human); 20296 (Mouse); 24770 (Rat); 403981 (Canine)
Alternate Names	C-C motif chemokine ligand 2, CCL2, GDCF-2, HC11, HSMCR30, MCAF, MCP-1, Mcp1, SCYA2, SMC-CF

Citations for Recombinant Rat CCL2/JE/MCP-1 Protein (7)

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Citations are publications that use Bio-Techne products. Selected citations for Recombinant Rat CCL2/JE/MCP-1 Protein include:

Species: Rat
Sample Types: In Vivo
Applications: In Vivo

H Tsushima et al. (2020), Effects of adipokine administration to the hypothalamic preoptic area on body temperature in rats J. Pharmacol. Sci., 0(0).
PMID: 32684333
Species: Rat
Sample Types: Whole Cells
Applications: Bioassay

X Ding et al. (2020), Serum IgG-induced microglial activation enhances neuronal cytolysis via the NO/sGC/PKG pathway in children with opsoclonus-myoclonus syndrome and neuroblastoma J Neuroinflammation, 17(1):190.
PMID: 32546235
Species: Rat
Sample Types: Whole Cells
Applications: Bioassay

HT Lee et al. (2017), A Crucial Role of CXCL14 for Promoting Regulatory T Cells Activation in Stroke Theranostics, 7(4):855-875.
PMID: 28382159
Species: Rat
Sample Types: Whole Cells
Applications: Bioassay

Nathalie Strazielle et al. (2016), T-Lymphocytes Traffic into the Brain across the Blood-CSF Barrier: Evidence Using a Reconstituted Choroid Plexus Epithelium. PLoS ONE, 0(0):1932-6203.
PMID: 26942913
Species: Rat
Sample Types: In Vivo
Applications: In Vivo

Huang C et al. (2014), Minocycline, a microglial inhibitor, blocks spinal CCL2-induced heat hyperalgesia and augmentation of glutamatergic transmission in substantia gelatinosa neurons. J Neuroinflammation, 11(0):7.
PMID: 24405660
Species: Rat
Sample Types: Whole Cells
Applications: Bioassay

Vomaske J et al. (2012), Cytomegalovirus CC chemokine promotes immune cell migration. J Virol, 86(21):11833-44.
PMID: 22915808
Species: Rat
Sample Types: Whole Cells
Applications: Bioassay

Bhangoo SK et al. (2007), CXCR4 chemokine receptor signaling mediates pain hypersensitivity in association with antiretroviral toxic neuropathy. Brain Behav. Immun., 21(5):581-91.
PMID: 17292584

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Recombinant Rat CCL2/JE/MCP-1 Protein

Name: Jagadeesh Janjanam

Application: Cell migration/motility

Verified Customer | Posted 06/19/2017

Recombinant Rat CCL2/JE/MCP-1 Protein

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Product Specifications for Recombinant Rat CCL2/JE/MCP-1 Protein

Formulation, Preparation and Storage

Carrier Free

3144-JE

Carrier

3144-JE/CF

Carrier Free

Reconstitution Calculator

Background: CCL2/JE/MCP-1

Citations for Recombinant Rat CCL2/JE/MCP-1 Protein (7)

Customer Reviews for Recombinant Rat CCL2/JE/MCP-1 Protein (1)

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Recombinant Rat CCL2/JE/MCP-1 Protein

Product Documents for Recombinant Rat CCL2/JE/MCP-1 Protein

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