Recombinant Rat B7-H4 Fc Chimera Protein, CF

Name: Recombinant Rat B7-H4 Fc Chimera Protein, CF
Brand: R&D Systems
SKU: 10085-B7

Catalog # 10085-B7

Product Datasheet / COA / SDS

Order Information

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Key Product Details

Source:	NS0
Accession #:	Q501W4
Structure / Form:	Disulfide-linked homodimer
Applications:	Bioactivity

View Full Technical Details for Recombinant Rat B7-H4 Fc Chimera Protein, CF

Product Specifications

Source

Mouse myeloma cell line, NS0-derived rat B7-H4 protein

Rat B7-H4 (Phe29-Gly257) Accession # Q501W4	IEGRMDP	Mouse IgG_2a (Glu98-Lys330)
N-terminus		C-terminus

Purity

>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

Endotoxin level

<0.10 EU per 1 μg of the protein by the LAL method.

N-terminal sequence Analysis

Phe29

Predicted Molecular Mass

52 kDa

SDS-PAGE

74-85 kDa, reducing conditions

Activity

Measured by its ability to inhibit anti-CD3 antibody induced IL-2 or IFN-gamma secretion by human T cells. The ED₅₀ for this effect is 1-6 μg/mL.

Recombinant Rat B7-H4 Fc Chimera Protein, CF Scientific Data Examples

	Recombinant Rat B7-H4 Fc Chimera Protein Bioactivity Recombinant Rat B7-H4 Fc Chimera (Catalog # 10085-B7) inhibits anti-CD3 antibody induced IFN-gamma secretion by human T cells. The ED₅₀ for this effect is 1-6 μg/mL.
	Recombinant Rat B7-H4 Fc Chimera Protein SDS-PAGE 2 μg/lane of Recombinant Rat B7‑H4 Fc Chimera (Catalog # 10085-B7) was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by Coomassie® Blue staining, showing bands at 74-85 kDa and 150-170 kDa, respectively.

Formulation, Preparation and Storage

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

10085-B7

Formulation	Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.
Reconstitution	Reconstitute at 500 μg/mL in PBS.
Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage:	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: B7-H4

B7-H4, also known as B7x, B7S1, and V-set domain-containing T-cell activation inhibitor 1, is a 50-80 kDa glycosylated member of the B7 family of immunomodulatory proteins (1-5). Mature rat B7-H4 consists of a 235 amino acid (aa) extracellular domain (ECD) with two Ig-like domains. Within the ECD, rat B7-H4 shares 90% and 99% aa sequence identity with human and mouse B7-H4, respectively. Alternate splicing of human B7-H4 generates an additional isoform that lacks the first Ig-like domain. B7-H4 is expressed on the surface of activated lymphocytes, macrophages, monocytes, dendritic cells, epithelial cells, and bone marrow-derived mesenchymal stem cells (4-8). Its binding to activated T cells dampens T cell responses and induces cell cycle arrest in the T cell (3-5). Reverse signaling can induce either cell cycle arrest or apoptosis in the B7-H4 expressing cell (9, 10). B7-H4 is up-regulated in several carcinomas in correlation with tumor progression and metastasis (2, 7, 11, 12). A soluble form of B7-H4 is elevated in the serum of ovarian cancer, renal cell carcinoma, and rheumatoid arthritis patients, also in correlation with advanced disease status (13-15). Soluble B7-H4 functions as a decoy molecule that blocks the inhibitory influence of B7-H4 on immune activation (15). Despite evidence for the involvement of B7-H4 in immune regulation, mice deficient in its expression do not show significant immune deficiencies, suggesting compensation by other molecules in vivo (16).

Item	Value
References	Yi, K.H. and L. Chen (2009) Immunol. Rev. 229:145. Salceda, S. et al. (2005) Exp. Cell Res. 306:128. Zang, X. et al. (2003) Proc. Natl. Acad. Sci. 100:10388. Prasad, V.R. et al. (2003) Immunity 18:863. Sica, G.L. et al. (2003) Immunity 18:849. Kryczek, I. et al. (2006) J. Exp. Med. 203:871. Tringler, B. et al. (2005) Clin. Cancer Res. 11:1842. Xue, Q. et al. (2010) Stem Cells Dev. 19:27. Song, H. et al. (2008) Cancer Lett. 266:227. Park, G.B. et al. (2009) Immunology 128:360. Zang, X. et al. (2007) Proc. Natl. Acad. Sci. 104:19458. Krambeck, A.E. et al. (2006) Proc. Natl. Acad. Sci. 103:10391. Simon, I. et al. (2006) Cancer Res. 66:1570. Thompson, R.H. et al. (2008) Cancer Res. 68:6054. Azuma, T. et al. (2009) PloS Med. 6:e1000166. Suh, W.-K., et al. (2006) Mol. Cell. Biol. 26:6403.
Long Name	B7 Homolog 4
Entrez Gene IDs	79679 (Human); 242122 (Mouse); 295322 (Rat)
Alternate Names	B7-H4, B7H4, B7H4T-cell costimulatory molecule B7x, B7S1, B7S1VCTN1, B7XPRO1291, B7h.5, B7x, FLJ22418, Immune costimulatory protein B7-H4, Protein B7S1, T cell costimulatory molecule B7x, V-set domain containing T cell activation inhibitor 1, V-set domain-containing T-cell activation inhibitor 1, Vtcn1

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