Recombinant Rat 4-1BB Ligand/TNFSF9 Protein

Name: Recombinant Rat 4-1BB Ligand/TNFSF9 Protein
Brand: R&D Systems
SKU: 9188-4L

R&D Systems, Inc. a Bio-Techne Brand

Catalog # 9188-4L

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Key Product Details

Source:	NS0
Accession #:	Q80WE6
Applications:	Bioactivity

View Full Technical Details for Recombinant Rat 4-1BB Ligand/TNFSF9 Protein

Product Specifications for Recombinant Rat 4-1BB Ligand/TNFSF9 Protein

Source	Mouse myeloma cell line, NS0-derived rat 4-1BB Ligand/TNFSF9 protein Arg103-Gly308, with an N-terminal 6-His tag
Purity	>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Endotoxin level	<0.10 EU per 1 μg of the protein by the LAL method.
N-terminal sequence Analysis	His
Predicted Molecular Mass	24 kDa
SDS-PAGE	33-43 kDa, reducing conditions
Activity	Measured by its binding ability in a functional ELISA. When Recombinant Rat 4-1BB/TNFRSF9 Fc Chimera (Catalog # 7968-4B) is Immobilized at 100 ng/mL (100 µL/well), the concentration of Recombinant Rat 4-1BB Ligand/TNFSF9 that produces 50% optimal binding response is approximately 1-6 ng/mL. Measured by its ability to co-stimulate IL-2 secretion by mouse T cells in the presence of anti-CD3. The ED₅₀ for for this effect is 5-30 ng/mL

Scientific Data Examples for Recombinant Rat 4-1BB Ligand/TNFSF9 Protein

	Recombinant Rat 4-1BB Ligand/TNFSF9 Protein Bioactivity When Recombinant Rat 4-1BB/TNFRSF9 Fc Chimera (Catalog # 7968-4B) is coated at 100 ng/mL, Recombinant Rat 4-1BB Ligand/TNFSF9 (Catalog # 9188-4L) binds with a ED₅₀ of 1-6 ng/mL.
	Recombinant Rat 4-1BB Ligand/TNFSF9 Protein Bioactivity Recombinant Rat 4-1BB Ligand (Catalog # 9188-4L) co-stimulates IL-2 secretion by mouse T cells in the presence of anti-CD3. The ED₅₀ for this effect is 5-30 ng/mL.

Formulation, Preparation and Storage

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

9188-4L

Carrier

Formulation	Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.
Reconstitution	Reconstitute at 250 μg/mL in PBS.
Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage:	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 3 months, -20 to -70 °C under sterile conditions after reconstitution.

9188-4L/CF

Carrier Free

Formulation	Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.
Reconstitution	Reconstitute at 250 μg/mL in PBS.
Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage:	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 3 months, -20 to -70 °C under sterile conditions after reconstitution.

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Background: 4-1BB Ligand/TNFSF9

4-1BB Ligand (4-1BBL), also known as CD137L, is a type II transmembrane protein that belongs to the TNF superfamily of molecules and plays an important role in immune response activation (1). The approximately 50 kDa rat 4-1BB Ligand consists of an 82 aa cytoplasmic domain, a 21 aa transmembrane segment, and a 206 aa extracellular domain (ECD) (2). Within the ECD, rat 4-1BB Ligand shares 34% and 86% aa sequence identity with human and mouse 4-1BB Ligand, respectively. 4-1BB Ligand is expressed by activated B cells, monocytes, macrophages, dendritic cells (DC), T cells, lymphoma and multiple myeloma cells, hematopoietic stem cells, early myeloid progenitors, neurons, and astrocytes (3-9). A 26 kDa soluble form of 4-1BB Ligand can be released from the surface of activated cells and retains bioactivity (10). 4-1BB Ligand binds to 4-1BB/TNFRSF9/CD137 on activated CD4⁺ and CD8⁺ T cells, thymocytes, and NK cells as well as on monocytes, neutrophils, DC, and eosinophils. In response to 4-1BB Ligand binding, 4-1BB transduces a co-stimulatory signal that promotes the proliferation, activation, and survival of CD4⁺ and CD8⁺ T cells (4, 11, 12). T cell co-stimulation through CD28 is important for the initial T cell expansion, while 4-1BB acts later in the response (12, 13). 4-1BB Ligand function supports the survival and responsiveness of memory T cells during viral infection (13-15). Reverse signaling through
4-1BB Ligand on monocytes induces the production of inflammatory cytokines (5). On macrophages, 4-1BB Ligand associates in cis with TLR4 and enhances inflammatory cytokine production in response to TLR4 ligation (6). Its expression on early myeloid progenitor cells limits the development of dendritic cells, monocytes, and B cells (9).

Item	Value
References	Wang, C. et al. (2009) Immunol. Rev. 229:192. Dong, Q.M. et al. (2005) Acta Biochim. Biophys. Sin. 37:694. Reali, C. et al. (2003) J. Neurosci. Res. 74:67. DeBenedette, M.A. et al. (1997) J. Immunol. 158:551. Langstein, J. et al. (1998) J. Immunol. 160:2488. Kang, Y.J. et al. (2007) Nat. Immunol. 8:601. Baessler, T. et al. (2010) Blood 115:3058. Gullo, C. et al. (2010) PLoS One 5:e10845. Lee, S.-W. et al. (2008) Nat. Immunol. 9:917. Salih, H.R. et al. (2001) J. Immunol. 167:4059. Wen, T. et al. (2002) J. Immunol. 168:4897. Cannons, J.L. et al. (2001) J. Immunol. 167:1313. Bertram, E.M. et al. (2002) J. Immunol. 168:3777. Bukczynski, J. et al. (2004) Proc. Natl. Acad. Sci. USA 101:1291. Lin, G.H.Y. et al. (2009) J. Immunol. 182:934.
Entrez Gene IDs	8744 (Human); 21950 (Mouse); 353218 (Rat); 102129676 (Cynomolgus Monkey)
Alternate Names	4-1BB Ligand, 4-1BB-L, 4-1BBL, 41BB Ligand, CD137L, TNFSF9, homolog of mouse 4-1BB-L, receptor 4-1BB ligand, tumor necrosis factor (ligand) superfamily, member 9, tumor necrosis factor ligand superfamily member 9

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Recombinant Rat 4-1BB Ligand/TNFSF9 Protein

R&D Systems, Inc. a Bio-Techne Brand

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Product Specifications for Recombinant Rat 4-1BB Ligand/TNFSF9 Protein

Scientific Data Examples for Recombinant Rat 4-1BB Ligand/TNFSF9 Protein

Formulation, Preparation and Storage

Carrier Free

9188-4L

Carrier

9188-4L/CF

Carrier Free

Reconstitution Calculator

Background: 4-1BB Ligand/TNFSF9

Citations for Recombinant Rat 4-1BB Ligand/TNFSF9 Protein

Product Documents for Recombinant Rat 4-1BB Ligand/TNFSF9 Protein

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