Recombinant Porcine IL-1 alpha/IL-1F1 Protein

Name: Recombinant Porcine IL-1 alpha/IL-1F1 Protein
Brand: R&D Systems
SKU: 680-PI

R&D Systems, Inc. a Bio-Techne Brand

Catalog # 680-PI

Citations (4)

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Source:	E. coli
Accession #:	P18430
Applications:	Bioactivity

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Product Specifications for Recombinant Porcine IL-1 alpha/IL-1F1 Protein

Source	E. coli-derived porcine IL-1 alpha/IL-1F1 protein Gln119-Ser270, with an N-terminal Met
Purity	>97%, by SDS-PAGE under reducing conditions and visualized by silver stain
Endotoxin level	<0.10 EU per 1 μg of the protein by the LAL method.
N-terminal sequence Analysis	Met
Predicted Molecular Mass	17.5 kDa
Activity	Measured in a cell proliferation assay using D10.G4.1 mouse helper T cells. Symons, J.A. et al. (1987) in Lymphokines and Interferons, a Practical Approach. Clemens, M.J. et al. (eds): IRL Press. 272. The ED₅₀ for this effect is 10-60 pg/mL.

Formulation, Preparation and Storage

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

680-PI

Carrier

Formulation	Lyophilized from a 0.2 μm filtered solution in PBS and DTT with BSA as a carrier protein.
Reconstitution	Reconstitute at 100 μg/mL in sterile PBS containing at least 0.1% human or bovine serum albumin.
Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage:	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 3 months, -20 to -70 °C under sterile conditions after reconstitution.

680-PI/CF

Carrier Free

Formulation	Lyophilized from a 0.2 μm filtered solution in PBS and DTT.
Reconstitution	Reconstitute at 100 μg/mL in sterile PBS.
Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage:	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 3 months, -20 to -70 °C under sterile conditions after reconstitution.

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The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: IL-1 alpha/IL-1F1

Interleukin 1 (IL-1) is a name that designates two proteins, IL-1 alpha and IL-1 beta, that are the products of distinct genes, but show approximately 25% amino acid sequence identity and recognize the same cell surface receptors. Although IL-1 production is generally considered to be a consequence of inflammation, evidence suggests that IL-1 is also temporarily upregulated during bone formation and the menstrual cycle and can be induced in response to nervous system stimulation. In response to stimuli produced by inflammatory agents, infections, or microbial endotoxins, a dramatic increase in the production of IL-1 by macrophages and various other cells is seen. Cells in particular known to produce IL-1 include osteoblasts, monocytes, macrophages, keratinocytes, Kupffer cells, hepatocytes, thymic and salivary gland epithelium, Schwann cells, fibroblasts and glia (oligodendroglia, astrocytes and microglia).

IL-1 alpha and IL-1 beta are both synthesized as 31 kDa precursors that are subsequently cleaved into proteins with molecular weights of approximately 17,000. Neither precursor contains a typical hydrophobic signal peptide sequence and most of the precursor form of IL-1 alpha remains in the cytosol of cells, although there is evidence for a membrane-bound form of the precursor form of IL-1 alpha. The IL-1 alpha precursor reportedly shows full biological activity in the EL-4 assay. Among various species, the amino acid sequence of mature IL-1 alpha is conserved 60% to 70% and porcine IL-1 has been found to be biologically active on murine cell lines. Both forms of IL-1 bind to the same receptors, designated as type I and type II. Evidence suggests that only the type I receptor is capable of signal transduction and that the type II receptor may function as a decoy, binding IL-1 and thus preventing the binding of IL-1 to the type I receptor.

Item	Value
References	Dower, S.K. and J.E. Sims, in Guidebook to Cytokines and their receptors (1994) N.A. Nicole ed. Oxford University Press NY p. 17.
Long Name	Interleukin 1 alpha
Entrez Gene IDs	3552 (Human); 16175 (Mouse); 24493 (Rat); 397094 (Porcine); 101926546 (Cynomolgus Monkey)
Alternate Names	BAF, Hematopoietin-1, IL-1 alpha, IL-1A, IL-1F1, IL1, IL1 alpha, IL1-ALPHA, IL1A, IL1F1, IL1F1hematopoietin-1, LAF, LEM, interleukin 1, alpha, interleukin-1 alpha, preinterleukin 1 alpha, pro-interleukin-1-alpha

Citations for Recombinant Porcine IL-1 alpha/IL-1F1 Protein (4)

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Citations are publications that use Bio-Techne products. Selected citations for Recombinant Porcine IL-1 alpha/IL-1F1 Protein include:

Species: Porcine
Sample Types: Whole Cells
Applications: Bioassay

Delputte PL et al. (2007), IFN-alpha treatment enhances porcine Arterivirus infection of monocytes via upregulation of the porcine Arterivirus receptor sialoadhesin. J. Interferon Cytokine Res., 27(9):757-66.
PMID: 17892397
Species: Porcine
Sample Types: Whole Cells
Applications: Bioassay

Kelley MJ et al. (2007), Synergism of TNF and IL-1 in the induction of matrix metalloproteinase-3 in trabecular meshwork. Invest. Ophthalmol. Vis. Sci., 48(6):2634-43.
PMID: 17525194
Species: Porcine
Sample Types: Whole Tissue
Applications: Bioassay

McNulty AL et al. (2007), Enhanced integrative repair of the porcine meniscus in vitro by inhibition of interleukin-1 or tumor necrosis factor alpha. Arthritis Rheum., 56(9):3033-42.
PMID: 17729298
Species: Porcine
Sample Types: Whole Cells
Applications: Bioassay

Pritchard S et al. (2006), Effects of interleukin-1 on calcium signaling and the increase of filamentous actin in isolated and in situ articular chondrocytes. Arthritis Rheum., 54(7):2164-74.
PMID: 16802354

Recombinant Porcine IL-1 alpha/IL-1F1 Protein

R&D Systems, Inc. a Bio-Techne Brand

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Product Specifications for Recombinant Porcine IL-1 alpha/IL-1F1 Protein

Formulation, Preparation and Storage

Carrier Free

680-PI

Carrier

680-PI/CF

Carrier Free

Reconstitution Calculator

Background: IL-1 alpha/IL-1F1

Citations for Recombinant Porcine IL-1 alpha/IL-1F1 Protein (4)

Product Documents for Recombinant Porcine IL-1 alpha/IL-1F1 Protein

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