Recombinant Porcine IFN-gamma Protein

Name: Recombinant Porcine IFN-gamma Protein
Brand: R&D Systems
SKU: 985-PI

Catalog # 985-PI | R&D Systems, Inc. a Bio-Techne Brand

Citations (16)

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Key Product Details

Source:	E. coli
Accession #:	P17803
Applications:	Bioactivity

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Technical Data
Citations (16) / Reviews

Product Specifications for Recombinant Porcine IFN-gamma Protein

Source	E. coli-derived porcine IFN-gamma protein Ser21-Lys166
Purity	>97%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Endotoxin level	<1.0 EU per 1 μg of the protein by the LAL method.
N-terminal sequence Analysis	Ser21
Predicted Molecular Mass	17.3 kDa
SDS-PAGE	17.3 kDa
Activity	Measured in an anti-viral assay using PK‑15 porcine kidney epithelial cells infected with encephalomyocarditis (EMC) virus. The ED₅₀ for this effect is 0.015‑0.045 ng/mL.

Formulation, Preparation and Storage

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

985-PI

Carrier

Formulation	Lyophilized from a 0.2 μm filtered solution in Sodium Succinate, Mannitol,Tween® 80 and TCEP with BSA as a carrier protein.
Reconstitution	Reconstitute at 50 μg/mL in sterile PBS containing at least 0.1% human or bovine serum albumin.
Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage:	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 3 months, -20 to -70 °C under sterile conditions after reconstitution.

985-PI/CF

Carrier Free

Formulation	Lyophilized from a 0.2 μm filtered solution in Sodium Succinate, Mannitol,Tween® 80 and TCEP.
Reconstitution	Reconstitute at 100 μg/mL in sterile PBS.
Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage:	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: IFN-gamma

Interferon-gamma (IFN-gamma ), also known as type II or immune interferon, exerts a wide range of immunoregulatory activities and is considered to be the prototype proinflammatory cytokine (1, 2). Mature porcine IFN-gamma exists as a noncovalently linked homodimer of 20-25 kDa variably glycosylated subunits (3). It shares 72%-79% amino acid sequence identity with bovine, canine, equine, and feline IFN-gamma and 41%-57% with cotton rat, human, mouse, rat, and rhesus IFN-gamma. IFN-gamma dimers bind to IFN-gamma RI ( alpha subunits) which then interact with IFN-gamma RII ( beta subunits) to form the functional receptor complex of two alpha and two beta subunits. Inclusion of IFN-gamma RII increases the binding affinity for ligand and the efficiency of signal transduction (4, 5). IFN-gamma is produced by a variety of immune cells under inflammatory conditions, notably by T cells and NK cells (6). It plays a key role in host defense by promoting the development and activation of Th1 cells, chemoattraction and activation of monocytes and macrophages, upregulation of antigen presentation molecules, and immunoglobulin class switching in B cells. It also exhibits antiviral, anti-proliferative, and apoptotic effects (6, 7). In addition, IFN-gamma functions as an anti-inflammatory mediator by promoting the development of regulatory T cells and inhibiting Th17 cell differentiation (8, 9). The pleiotropic effects of IFN-gamma contribute to the development of multiple aspects of atherosclerosis (7).

Item	Value
References	Billiau, A. and P. Matthys (2009) Cytokine Growth Factor Rev. 20:97. Pestka, S. et al. (2004) Immunol. Rev. 202:8. Dijkmans, R. et al. (1990) Nucl. Acids Res. 18:4259. Marsters, S.A. et al. (1995) Proc. Natl. Acad. Sci. 92:5401. Krause, C.D. et al. (2000) J. Biol. Chem. 275:22995. Schroder, K. et al. (2004) J. Leukoc. Biol. 75:163. McLaren, J.E. and D.P. Ramji (2009) Cytokine Growth Factor Rev. 20:125. Muhl, H. and J. Pfeilschifter (2003) Int. Immunopharmacol. 3:1247. Kelchtermans, H. et al. (2008) Trends Immunol. 29:479.
Long Name	Interferon gamma
Entrez Gene IDs	3458 (Human); 15978 (Mouse); 25712 (Rat); 396991 (Porcine); 281237 (Bovine); 403801 (Canine); 493965 (Feline)
Alternate Names	IFG, IFI, IFN-gamma, IFNG, IFNgamma, Immune interferon, interferon gamma, interferon, gamma

Citations for Recombinant Porcine IFN-gamma Protein (16)

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Citations are publications that use Bio-Techne products. Selected citations for Recombinant Porcine IFN-gamma Protein include:

Species: Porcine
Sample Types: Whole Cells
Applications: Bioassay

Z Oreskovic et al. (2022-02-06), Effects of IFNgamma and IL4 rich microenvironment on porcine monocyte-derived dendritic cell activation in vitro Research in Veterinary Science, 2022-0145(0):54-62.
PMID: 35168110
Species: Porcine
Sample Types: Whole Cells
Applications: Cell Culture

P Li et al. (2021-06-23), Genetic engineering of porcine endothelial cell lines for evaluation of human-to-pig xenoreactive immune responses Scientific Reports, 2021-011(1):13131.
PMID: 34162938
Species: Porcine
Sample Types: Whole Cells
Applications: Cell Culture

T Cao et al. (2019-10-31), Npro of Classical Swine Fever Virus Suppresses Type III Interferon Production by Inhibiting IRF1 Expression and Its Nuclear Translocation Viruses, 2019-111(11).
PMID: 31683525
Species: Porcine
Sample Types: In Vivo
Applications: In Vivo

T Huang et al. (2019-06-11), MicroRNA responses associated with Salmonella enterica serovar typhimurium challenge in peripheral blood: effects of miR-146a and IFN-gamma in regulation of fecal bacteria shedding counts in pig BMC Vet. Res., 2019-015(1):195.
PMID: 31186019
Species: Porcine
Sample Types: Whole Cells
Applications: Bioassay

G Ogno et al. (2019-03-11), In vitro characterization of PRRSV isolates with different in vivo virulence using monocyte-derived macrophages Vet. Microbiol., 2019-0231(0):139-146.
PMID: 30955801
Species: Porcine
Sample Types: Whole Tissue
Applications: Bioassay

I Yoo et al. (2018-11-11), Analysis of stage-specific expression of the toll-like receptor family in the porcine endometrium throughout the estrous cycle and pregnancy Theriogenology, 2018-1125(0):173-183.
PMID: 30448720
Species: Porcine
Sample Types: Whole Cells
Applications: Bioassay

H Singleton et al. (2018-03-02), Infection of monocytes with European porcine reproductive and respiratory syndrome virus (PRRSV-1) strain Lena is significantly enhanced by dexamethasone and IL-10 Virology, 2018-00(0).
PMID: 29502802
Species: Porcine
Sample Types: Whole Cells
Applications: Bioassay

MD de Smet et al. (2018-01-01), A Subretinal Cell Delivery Method via Suprachoroidal Access in Minipigs: Safety and Surgical Outcomes Invest. Ophthalmol. Vis. Sci., 2018-059(1):311-320.
PMID: 29346488
Species: Porcine
Sample Types: Whole Cells
Applications: Bioassay

J Nau et al. (2017-08-13), Experimental Porcine Toxoplasma gondii Infection as a Representative Model for Human Toxoplasmosis Mediators Inflamm., 2017-02017(0):3260289.
PMID: 28883687
Species: Porcine
Sample Types: Whole Tissue
Applications: Bioassay

Hakhyun Ka (2016-09-22), Characterization of interferon ? and ? receptor IFNAR1 and IFNAR2 expression and regulation in the uterine endometrium during the estrous cycle and pregnancy in pigs Theriogenology, 2016-088(0):166-173.
PMID: 27769575
Species: Porcine
Sample Types: Whole Cells
Applications: Bioassay

Sang Y et al. (2014-02-05), Genome-wide analysis of antiviral signature genes in porcine macrophages at different activation statuses. PLoS ONE, 2014-09(2):e87613.
PMID: 24505295
Species: Porcine
Sample Types: Whole Cells
Applications: Bioassay

Lannes N et al. (2013-04-08), Regulation of porcine plasmacytoid dendritic cells by cytokines. PLoS ONE, 2013-08(4):e60893.
PMID: 23577175
Species: Porcine
Sample Types: Whole Cells
Applications: Bioassay

Delputte PL et al. (2007-09-01), IFN-alpha treatment enhances porcine Arterivirus infection of monocytes via upregulation of the porcine Arterivirus receptor sialoadhesin. J. Interferon Cytokine Res., 2007-027(9):757-66.
PMID: 17892397
Species: Porcine
Sample Types: Whole Cells
Applications: Bioassay

Poncelet AJ et al. (2007-03-27), Although pig allogeneic mesenchymal stem cells are not immunogenic in vitro, intracardiac injection elicits an immune response in vivo. Transplantation, 2007-083(6):783-90.
PMID: 17414713
Species: Porcine
Sample Types: Whole Cells
Applications: Bioassay

Tambuyzer BR et al. (2005-08-21), Inhibition of microglia multinucleated giant cell formation and induction of differentiation by GM-CSF using a porcine in vitro model. Cytokine, 2005-031(4):270-9.
PMID: 16009563
Species: Porcine
Sample Types: Whole Cells
Applications: Bioassay

Seo SH et al. (2004-07-01), The NS1 gene of H5N1 influenza viruses circumvents the host anti-viral cytokine responses. Virus Res., 2004-0103(1):107-13.
PMID: 15163498

Recombinant Porcine IFN-gamma Protein

Catalog # 985-PI | R&D Systems, Inc. a Bio-Techne Brand

Key Product Details

Product Specifications for Recombinant Porcine IFN-gamma Protein

Formulation, Preparation and Storage

Carrier Free

985-PI

Carrier

985-PI/CF

Carrier Free

Reconstitution Calculator

Background: IFN-gamma

Citations for Recombinant Porcine IFN-gamma Protein (16)

Product Documents for Recombinant Porcine IFN-gamma Protein

Product Datasheets

COA

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