Recombinant P. heparinus Chondroitinase B Protein, CF

R&D Systems, part of Bio-Techne | Catalog # 6974-GH

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6974-GH-020
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Key Product Details

  • R&D Systems E. coli-derived Recombinant P. heparinus Chondroitinase B Protein (6974-GH)
  • Quality control testing to verify active proteins with lot specific assays by in-house scientists
  • All R&D Systems proteins are covered with a 100% guarantee

Source

E. coli

Accession #

Q46079

Conjugate

Unconjugated

Applications

Enzyme Activity

View all Chondroitin B Lyase/Chondroitinase B Proteins and Enzymes »

Product Specifications

Source

E. coli-derived p. heparinus Chondroitin B Lyase/Chondroitinase B protein
Gln26-His506, with an N-terminal Met and 6-His tag

Purity

>95%, by SDS-PAGE under reducing conditions and visualized by Colloidal Coomassie® Blue stain at 5 μg per lane.

Endotoxin Level

<1.0 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

Inconclusive results, Met predicted. Protein identity confirmed by MS analysis of tryptic fragments.

Predicted Molecular Mass

55 kDa

SDS-PAGE

52-56 kDa, reducing conditions

Activity

Measured by its ability to hydrolyze chondroitin sulfate B.
The specific activity is >90,000 pmol/min/μg, as measured under the described conditions.

Formulation, Preparation and Storage

6974-GH
Formulation Supplied as a 0.2 μm filtered solution in Tris and NaCl.
Shipping The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 6 months from date of receipt, -20 to -70 °C as supplied.
  • 3 months, -20 to -70 °C under sterile conditions after opening.

Background: Chondroitin B Lyase/Chondroitinase B

Chondroitinase B from P. heparinus is a depolymerizing lyase that is especially active on the sulfated polysaccharide dermatan sulfate (DS) (1, 2). DS is a sulfated polysaccharide that is abundantly located in the skin, but is also present in the blood vessels, heart valves, tendons, and lungs. DS may play roles in coagulation, cardiovascular disease, carcinogenesis, infection, wound repair, and fibrosis (3). DS with a repeating disaccharide unit of IdoA alpha1-3GlcNAc is most closely related to chondroitin sulfate (CS) that has a repeating disaccharide unit of GlcA beta1-3GlcNAc. Due to this similarity, DS was originally referred to as chondroitin sulfate B, and the DS-depolymerizing enzyme was named Chondroitinase B. DS contains more sulfate than CS at levels comparable to the anticoagulant drug heparin, which is the reason that purified commercial heparin is more likely to be contaminated by DS. Chondroitinase B can be used to specifically degrade and distinguish DS in glycosaminoglycan preparations (4, 5, 6).

References

  1. Oike, Y. et al. (1980) Biochem. J. 191:193.
  2. Prabhakar, V. et al. (2009) J. Biol. Chem. 284:974.
  3. Trowbridge, J.M. and Gallo, R.L. (2002) Glycobiology 12:117R.
  4. Gu, K. et al. (1995) Biochem. J. 312:356.
  5. Tkalec, A.L. et al. (2000) Appl. Environ. Microbiol. 66:29.
  6. Wu, Z.L. et al. (2011) Glycobiology 21:625.

Alternate Names

Chondroitinase B

Entrez Gene IDs

8251878 (P. heparinus)

Gene Symbol

PHEP_0789

UniProt

Q46079

Additional Chondroitin B Lyase/Chondroitinase B Products

Product Documents for Recombinant P. heparinus Chondroitinase B Protein, CF

Product Datasheets

Certificate of Analysis

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Product Specific Notices for Recombinant P. heparinus Chondroitinase B Protein, CF

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