Recombinant P. heparinus Chondroitinase AC Protein, CF Best Seller

R&D Systems, part of Bio-Techne | Catalog # 8384-GH

R&D Systems, part of Bio-Techne
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Key Product Details

Source

E. coli

Accession #

Q59288

Conjugate

Unconjugated

Applications

Enzyme Activity

View all Chondroitinase AC Proteins and Enzymes »

Product Specifications

Source

E. coli-derived p. heparinus Chondroitinase AC protein
Gln23-Lys700 with an N-terminal Met and 6-His tag

Purity

>95%, by SDS-PAGE under reducing conditions and visualized by Colloidal Coomassie® Blue stain at 5 μg per lane.

Endotoxin Level

<0.01 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

Met

Predicted Molecular Mass

78 kDa

SDS-PAGE

66 kDa, reducing conditions

Activity

Measured by its ability to hydrolyze chondroitin sulfate.
The specific activity is >50,000 pmol/min/μg, as measured under the described conditions.

Formulation, Preparation and Storage

8384-GH
Formulation Supplied as a 0.2 μm filtered solution in Tris, NaCl and Brij-35.
Shipping The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 6 months from date of receipt, -20 to -70 °C as supplied.
  • 3 months, -20 to -70 °C under sterile conditions after opening.

Background: Chondroitinase AC

Chondroitinase AC from P. heparinum is a depolymerizing lyase that degrades chondroitin sulfates A and C, but not chondroitin sulfate B (1). Both chondroitin sulfate A and C contain D-glucuronic acid, N-acetylgalactosamine, and sulfate residues in equal molar quantities (2). The sulfate ester is at the 4-O position of N-acetylgalactosamine residues on chondroitin sulfate A and at the 6-O position of N-acetylgalactosamine residues on chondroitin sulfate C. In contrast chondroitin sulfate B contains L-iduronic acid instead of glucuronic acid (3). Chondroitinase AC cleaves, via an elimination mechanism, chondroitin sulfate chains between N-acetylgalactosamine and glucuronic acid residues. The reaction yields oligosaccharide products, primarily disaccharides, containing unsaturated uronic acids that can be detected by UV spectroscopy. The enzyme shows approximately equal activity with chondroitin sulfates A orC (3). Chondrotinase AC can be used to specifically degrade chondroitin A and C and distinguish among different species of glycosaminoglycans (4).

References

  1. Tkalec, A.L. et al. (2000) Appl. Environ. Microbiol. 66:29.
  2. Saito, H., et al. (1968) J. Biol. Chem. 243:1536.
  3. Gu, K. et al. (1995) Biochem. J. 312:569.
  4. Wu, Z.L. et al. (2011) Glycobiology 21:625.

Entrez Gene IDs

8251875 (P. heparinus)

Gene Symbol

PHEP_0786

UniProt

Q59288

Additional Chondroitinase AC Products

Product Documents for Recombinant P. heparinus Chondroitinase AC Protein, CF

Product Datasheets

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Product Specific Notices for Recombinant P. heparinus Chondroitinase AC Protein, CF

For research use only

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