Recombinant Mouse WISP-1/CCN4 Protein, CF

Catalog # 1680-WS | R&D Systems, Inc. a Bio-Techne Brand
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Key Product Details

Accession #

O54775

Source

NS0

Conjugate

Unconjugated

Applications

Bioactivity

Product Specifications

Source

Mouse myeloma cell line, NS0-derived mouse WISP-1/CCN4 protein
Thr23-Asn367, with a C-terminal 10-His tag

Purity

>90%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

Thr23

Predicted Molecular Mass

39.6 kDa

SDS-PAGE

65 kDa, reducing conditions

Activity

Measured by its ability to inhibit the cell growth of DU145 human prostate carcinoma cells.
The ED50 for this effect is 1.5 -9 µg/mL.

Formulation, Preparation and Storage

1680-WS
Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution
Reconstitute at 500 μg/mL in sterile PBS.

Reconstitution Buffer Available:
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Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: WISP-1/CCN4

Mouse WISP-1 (Wnt-induced secreted protein-1; also named CNN4) is a 40 kDa, secreted, heparin-binding glycoprotein that is a member of the CCN (or CTGF/Cyr61/Nov) cysteine-rich protein family (1-6). It is synthesized as a 367 amino acid (aa) precursor that contains a series of structural homology modules. Following a 22 aa signal sequence, there is a 68 aa IGFBP-like domain (aa 53-120), a 66 aa von Willebrand factor type C (VWC) module (aa 121-186), a 41 aa TSP type I domain (aa 220-260) and a 75 aa, C-terminal cysteine knot motif (aa 273-347) (1, 6). The VWC module is associated with protein-protein interaction, the TSP domain binds sulfated glycoconjugates, and the cysteine knot mediates dimerization and receptor binding (4). It is likely that WISP-1 normally circulates as an 80 kDa homodimer (2). Multiple splice forms are reported for human WISP-1, but none for rodent (7-9). Mature mouse WISP-1 is 98%, 84% and 85% aa identical to rat, canine and human WISP-1, respectively. WISP-1 is expressed by fibroblasts and osteoblasts, and may contribute to fracture healing by promoting bone cell formation (1, 10, 11).

References

  1. Pennica, D. et al. (1998) Proc. Natl. Acad. Sci. USA 95:14717.
  2. Tanada, S. et al. (2001) Oncogene 20:5525.
  3. Brigstock, D.R. et al. (2003) J. Clin. Pathol. Mol. Pathol. 56:127.
  4. Desnoyers, L. (2004) Curr. Pharm. Des. 10:3913.
  5. Brigstock, D.R. (2003) J. Endocrinol. 178:169.
  6. Hashimoto, Y. et al. (1998) J. Exp. Med. 187:289.
  7. SwissProt Accession # O54775.
  8. SwissProt Accession # Q99PP0.
  9. SwissProt Accession # O95388
  10. French, D.M. et al. (2004) Am. J. Pathol. 165:855.
  11. Parisi, M.S. et al. (2006) Bone 38:671.

Long Name

Wnt-1 Induced Secreted Protein 1

Alternate Names

CCN4, WISP1

Entrez Gene IDs

8840 (Human); 22402 (Mouse); 102125044 (Cynomolgus Monkey)

Gene Symbol

CCN4

UniProt

O54775

Product Documents for Recombinant Mouse WISP-1/CCN4 Protein, CF

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Recombinant Mouse WISP-1/CCN4 Protein, CF

For research use only

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