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Recombinant Mouse WIF-1 Protein, CF

Catalog # 135-WF | R&D Systems, Inc. a Bio-Techne Brand
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135-WF-050

Key Product Details

Source

NS0

Accession #

Conjugate

Unconjugated

Applications

Bioactivity

Product Specifications

Source

Mouse myeloma cell line, NS0-derived mouse WIF-1 protein
Mouse WIF-1
(Gly29-Trp379)
Accession # Q9WUA1
HIEGR GGGSGGGSGGGS  10-His tag
N-terminus C-terminus

Purity

>90%, by SDS-PAGE under reducing conditions and visualized by silver stain.

Endotoxin Level

<0.01 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

Gly29

Predicted Molecular Mass

41.1 kDa

SDS-PAGE

50-55 kDa, reducing conditions

Activity

Measured by its ability to inhibit Wnt-3a-induced alkaline phosphatase production by MC3T3‑E1 mouse preosteoblast cells.
The ED50 for this effect is 0.7-3.5 μg/mL.

Formulation, Preparation and Storage

135-WF
Formulation Lyophilized from a 0.2 μm filtered solution in Acetic Acid, NaCl, PEG3350 and CHAPS, pH5.0.
Reconstitution
Reconstitute at 500 μg/mL in sterile, deionized water.  .

Reconstitution Buffer Available:
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Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: WIF-1

WIF-1 (Wnt Inhibitory Factor-1) is a secreted Wnt inhibitor. Like sFRPs (secreted Frizzled-related proteins), WIF-1 binds to Wnt proteins and inhibits their receptor binding, thus inhibiting all Wnt signaling pathways (1, 2). WIF-1 is synthesized as a 379 amino acid (aa) precursor that contains a 28 aa N-terminal signal sequence, a WIF domain (aa 38-177), 5 EGF-like repeats (aa 178-338), and a C-terminal hydrophilic domain (1). Mature mouse WIF-1 shares 95%, 99%, 87% and 85% aa sequence identity with human, rat, equine and Xenopus WIF-1, respectively. A potential 220 aa isoform with an alternate N-terminus that lacks the WIF domain is identical with the 379 aa form after aa 179 (3).  The WIF domain contains a phospholipid binding site that interacts with lipid-modified Wnt molecules, while a binding site for sulfated proteoglycans within the EGF repeats contributes to binding and inhibition (4). WIF-1 fine-tunes Wnt signaling by time- and location-specific expression in the fetal and postnatal mouse, especially during joint development and cardiomyogenesis (5-7). Binding of Wnts 3a, 4, 5, 7a, 9a and 11, with little or no binding to Wnts 5b, 7b, and 9b, has been shown (5). Binding and inhibition of other molecules in cartilage, such as CTGF (connective tissue growth factor), has also been shown (6). WIF-1 expression is up‑regulated by BMP-4/ Smad1 signaling during fetal lung development (8). WIF-1 functions as a tumor suppressor in cancers of the prostate, breast, lung, bladder, cervix, liver and brain (9-12). Its down‑regulation by promoter hypermethylation is detected in a variety of tumors (11-13). WIF‑1 anti‑tumor effects include antagonizing angiogenesis, growth and invasion, and inducing apoptosis, senescence, or differentiation (2, 10-13). WIF-1 has been used experimentally to antagonize tumors or regulate developmental Wnt activities (10-15).

References

  1. Hsieh, J-C. et al. (1999) Nature 398:431.
  2. Zhang, B. and J. Ma (2010) Protein Cell 1:898.
  3. GenBank Accession # AAH04048.
  4. Malinauskas, T. et al. (2011) Nat. Struct. Mol. Biol. 18:886.
  5. Surmann-Schmitt, C. et al. (2009) J. Cell Sci. 122:3627.
  6. Surmann-Schmitt, C. et al. (2011) J. Cell Physiol. Sept. 16 [Epub ahead of print]
  7. Buermans, H.P.J. et al. (2010) PLoS ONE 5:e15504.
  8. Xu, B. et al. (2011) Development 138:925.
  9. Wissmann, C. et al. (2003) J. Pathol 201:204.
  10. Hu, J. et al. (2009) Cancer Res. 69:6951.
  11. Ramachandran, I. et al. (2011) Oncogene Oct. 17 [Epub ahead of print]
  12. Lambiv, W.L. et al. (2011) Neuro. Oncol. 13:736.
  13. Kansara, M. et al. (2009) J. Clin. Invest. 119:837.
  14. Yee, D.S. et al. (2010) Mol. Cancer 9:162.
  15. Schaniel, C. et al. (2011) Blood 118:2420.

Long Name

Wnt Inhibitory Factor

Alternate Names

WIF1

Entrez Gene IDs

11197 (Human); 24117 (Mouse)

Gene Symbol

WIF1

UniProt

Additional WIF-1 Products

Product Documents for Recombinant Mouse WIF-1 Protein, CF

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

Product Specific Notices for Recombinant Mouse WIF-1 Protein, CF

For research use only

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