Recombinant Mouse VSTM2B Fc Chimera Protein, CF

Name: Recombinant Mouse VSTM2B Fc Chimera Protein, CF
Brand: rnd
SKU: 10076-VT

Catalog # 10076-VT

Datasheet / COA / SDS

Order Information

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Key Product Details

Source:	NS0
Accession #:	Q9JME9
Structure / Form:	Disulfide-linked homodimer
Applications:	Bioactivity

View Full Technical Details for Recombinant Mouse VSTM2B Fc Chimera Protein, CF

Product Specifications

Source

Mouse myeloma cell line, NS0-derived mouse VSTM2B protein

Mouse VSTM2B (Thr29-Ser263) Accession # Q9JME9	IEGRMD	Human IgG₁ (Pro100-Lys330)
N-terminus		C-terminus

Purity

>90%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

Endotoxin level

<0.10 EU per 1 μg of the protein by the LAL method.

N-terminal sequence Analysis

Thr29

Predicted Molecular Mass

51 kDa

SDS-PAGE

61-70 kDa, reducing conditions

Activity

Measured by its ability to inhibit anti-CD3 antibody induced IFN-gamma secretion by human peripheral blood mononuclear cells (PBMC). The ED₅₀ for this effect is 2-10 μg/mL.

Recombinant Mouse VSTM2B Fc Chimera Protein, CF Scientific Data Examples

	Recombinant Mouse VSTM2B Fc Chimera Protein Bioactivity Recombinant Mouse VSTM2B Fc Chimera (Catalog # 10076-VT) inhibits IFN-gamma secretion by human peripheral blood mononuclear cells in the presence of anti-CD3 antibody. The ED₅₀ for this effect is 2-10 μg/mL.
	Recombinant Mouse VSTM2B Fc Chimera Protein SDS-PAGE 2 μg/lane of Recombinant Mouse VSTM2B Fc Chimera was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by Coomassie® Blue staining, showing bands at 61-70 kDa and 120-140 kDa, respectively.

Formulation, Preparation and Storage

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

10076-VT

Formulation	Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution	Reconstitute at 200 μg/mL in PBS.
Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage:	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 3 months, ≤ -20 °C under sterile conditions after reconstitution.

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Desired Reconstitution Concentration

Background: VSTM2B

V-set and transmembrane domain-containing protein 2B, also known as VSTM-2B, is a single pass Type I membrane protein. Mouse VSTM-2B contains an N-Terminal signal peptide (1-28 aa), an extracellular domain (aa 29-263) which contains a single Ig-like V-Type domain (aa 29-143) with a single disulfide bond between C49 and C127, a helical transmembrane domain (aa 264-284) and a single histidine in the cytoplasm (aa 285). VSTM2B is expressed in multiple organs, with the highest expression in the pyloric antrum. The extracellular domain shares 89% and 96% sequence identity for the human and rat homolog, respectively. Currently, both the expression pattern and biological functions of VSTM2B remain unknown. our in house data show that VSTM2B inhibits anti-CD3 induced IL-2 and IFN-g secretion on human T cells.

Item	Value
Long Name	V-Set And Transmembrane Domain Containing 2B
Entrez Gene IDs	342865 (Human); 58188 (Mouse); 361560 (Rat)
Alternate Names	V-set and transmembrane domain-containing protein 2B, VSTM2B

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