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Recombinant Mouse VSTM2B Fc Chimera Protein, CF

Catalog # 10076-VT | R&D Systems, Inc. a Bio-Techne Brand
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10076-VT-050

Key Product Details

Source

NS0

Accession #

Structure / Form

Disulfide-linked homodimer

Conjugate

Unconjugated

Applications

Bioactivity

Product Specifications

Source

Mouse myeloma cell line, NS0-derived mouse VSTM2B protein
Mouse VSTM2B
(Thr29-Ser263)
Accession # Q9JME9
IEGRMD Human IgG1
(Pro100-Lys330)
N-terminus C-terminus

Purity

>90%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

Thr29

Predicted Molecular Mass

51 kDa

SDS-PAGE

61-70 kDa, reducing conditions

Activity

Measured by its ability to inhibit anti-CD3 antibody induced IFN-gamma secretion by human peripheral blood mononuclear cells (PBMC).
The ED50 for this effect is 2-10 μg/mL.

Scientific Data Images for Recombinant Mouse VSTM2B Fc Chimera Protein, CF

Recombinant Mouse VSTM2B Fc Chimera Protein Bioactivity

Recombinant Mouse VSTM2B Fc Chimera Protein Bioactivity

Recombinant Mouse VSTM2B Fc Chimera (Catalog # 10076-VT) inhibits IFN-gamma secretion by human peripheral blood mononuclear cells in the presence of anti-CD3 antibody. The ED50 for this effect is 2-10 μg/mL.
Recombinant Mouse VSTM2B Fc Chimera Protein SDS-PAGE

Recombinant Mouse VSTM2B Fc Chimera Protein SDS-PAGE

2 μg/lane of Recombinant Mouse VSTM2B Fc Chimera was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by Coomassie® Blue staining, showing bands at 61-70 kDa and 120-140 kDa, respectively.

Formulation, Preparation and Storage

10076-VT
Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution Reconstitute at 200 μg/mL in PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, ≤ -20 °C under sterile conditions after reconstitution.

Background: VSTM2B

V-set and transmembrane domain-containing protein 2B, also known as VSTM-2B, is a single pass Type I membrane protein. Mouse VSTM-2B contains an N-Terminal signal peptide (1-28 aa), an extracellular domain (aa 29-263) which contains a single Ig-like V-Type domain (aa 29-143) with a single disulfide bond between C49 and C127, a helical transmembrane domain (aa 264-284) and a single histidine in the cytoplasm (aa 285). VSTM2B is expressed in multiple organs, with the highest expression in the pyloric antrum. The extracellular domain shares 89% and 96% sequence identity for the human and rat homolog, respectively. Currently, both the expression pattern and biological functions of VSTM2B remain unknown. our in house data show that VSTM2B inhibits anti-CD3 induced IL-2 and IFN-g secretion on human T cells.

Long Name

V-Set And Transmembrane Domain Containing 2B

Alternate Names

V-set and transmembrane domain-containing protein 2B

Entrez Gene IDs

342865 (Human); 58188 (Mouse); 361560 (Rat)

Gene Symbol

VSTM2B

UniProt

Product Documents for Recombinant Mouse VSTM2B Fc Chimera Protein, CF

Certificate of Analysis

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Product Specific Notices for Recombinant Mouse VSTM2B Fc Chimera Protein, CF

For research use only

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