Recombinant Mouse VEGF 120 Protein

Name: Recombinant Mouse VEGF 120 Protein
Brand: R&D Systems
SKU: 494-VE
Price: 340 USD

R&D Systems, Inc. a Bio-Techne Brand

Catalog # 494-VE

Citations (8)

Product Datasheet / COA / SDS

Catalog #

Availability

Size / Price

Qty

Setting the standard in quality research reagents

Key Product Details

Source:	E. coli
Accession #:	AAB22254
Structure / Form:	Disulfide-linked homodimer
Applications:	Bioactivity

View Full Technical Details for Recombinant Mouse VEGF 120 Protein

Product Specifications for Recombinant Mouse VEGF 120 Protein

Source	E. coli-derived mouse VEGF protein Ala27-Arg146 (Gly117Glu & Glu118Arg)
Purity	>97%, by SDS-PAGE under reducing conditions and visualized by silver stain.
Endotoxin level	<0.10 EU per 1 μg of the protein by the LAL method.
N-terminal sequence Analysis	Ala27
Predicted Molecular Mass	14 kDa
Activity	Measured in a cell proliferation assay using HUVEC human umbilical vein endothelial cells. The ED₅₀ for this effect is 1-4 ng/mL.

Formulation, Preparation and Storage

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

494-VE

Carrier

Formulation	Lyophilized from a 0.2 μm filtered solution in Acetonitrile and TFA with BSA as a carrier protein.
Reconstitution	Reconstitute at 50 μg/mL in sterile PBS containing at least 0.1% human or bovine serum albumin.
Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage:	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 3 months, -20 to -70 °C under sterile conditions after reconstitution.

494-VE/CF

Carrier Free

Formulation	Lyophilized from a 0.2 μm filtered solution in Acetonitrile and TFA.
Reconstitution	Reconstitute at 50 µg/mL in sterile PBS.
Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage:	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

Volume (to add to vial)

Mass (in vial)

Desired Reconstitution Concentration

Background: VEGF

Vascular endothelial growth factor (VEGF or VEGF-A), also known as vascular permeability factor (VPF), is a potent mediator of both angiogenesis and vasculogenesis in the fetus and adult (1‑3). It is a member of the PDGF family that is characterized by a cysteine-knot structure formed by eight conserved cysteine residues (4). Alternately spliced isoforms of 121, 145, 165, 183, 189, and 206 amino acids (aa) have been identified in humans, with 120, 164 and 188 aa isoforms found in mouse (2, 4). VEGF₁₂₀ and VEGF₁₂₁ lack basic heparin-binding regions and are freely diffusible (4). Mouse embryos expressing only the VEGF₁₂₀ isoform do not survive to term and show defects in skeletogenesis (5). Mouse VEGF₁₂₀ shares 98% aa sequence identity with corresponding regions of rat, 89% with canine, feline, equine and porcine, and 87% with human, ovine and bovine VEGF, respectively. VEGF binds the type I transmembrane receptor tyrosine kinases VEGF R1 (also called Flt-1) and VEGF R2 (Flk-1/KDR) on endothelial cells (4). Although affinity is highest for binding to VEGF R1, VEGF R2 appears to be the primary mediator of VEGF angiogenic activity (3, 4). Human VEGF₁₆₅ binds the semaphorin receptor, Neuropilin-1; VEGF₁₂₁ binding has also been reported (6). VEGF is required during embryogenesis to regulate the proliferation, migration, and survival of endothelial cells (3, 4). In adults, VEGF functions mainly in wound healing and the female reproductive cycle (3). Pathologically, it is involved in tumor angiogenesis and vascular leakage (7, 8). Circulating VEGF levels correlate with disease activity in autoimmune diseases such as rheumatoid arthritis, multiple sclerosis and systemic lupus erythematosus (9). VEGF is induced by hypoxia and cytokines such as IL‑1, IL-6, IL-8, oncostatin M and TNF-alpha (3, 4, 10).

Item	Value
References	Breier, G. et al. (1992) Development 114:521. Shima, D.T. et al. (1996) J. Biol. Chem. 271:3877. Byrne, A.M. et al. (2005) J. Cell. Mol. Med. 9:777. Robinson, C.J. and S.E. Stringer (2001) J. Cell. Sci. 114:853. Zelzer, E. et al. (2002) Development 129:1893. Pan, Q. et al. (2007) J. Biol. Chem. 282:24049. Weis, S.M. and D.A. Cheresh (2005) Nature 437:497. Thurston, G. (2002) J. Anat. 200:575. Carvalho, J.F. et al. (2007) J. Clin. Immunol. 27:246. Angelo, L.S. and R. Kurzrock (2007) Clin. Cancer Res. 13:2825.
Long Name	Vascular Endothelial Growth Factor
Entrez Gene IDs	7422 (Human); 22339 (Mouse); 83785 (Rat); 281572 (Bovine); 403802 (Canine); 493845 (Feline); 30682 (Zebrafish)
Alternate Names	MVCD1, VAS, VEGF, VEGF-A, VEGFA, VEGFMGC70609, VPF, VPFvascular endothelial growth factor, Vascular permeability factor, Vasculotropin, vascular endothelial growth factor A

Citations for Recombinant Mouse VEGF 120 Protein (8)

Showing 1 - 8 of 8 citations Showing All

Filter By:

Search Title:

Citations are publications that use Bio-Techne products. Selected citations for Recombinant Mouse VEGF 120 Protein include:

Species: N/A
Sample Types: Recombinant Protein
Applications: Western Blot

Y Ichiyama et al. (2022), Assessment of mouse VEGF neutralization by ranibizumab and aflibercept PLoS ONE, 17(12):e0278951.
PMID: 36542626
Species: Mouse
Sample Types: Organoid
Applications: Bioassay

J Wang et al. (2021), Endothelial Wnts control mammary epithelial patterning via fibroblast signaling Cell Reports, 34(13):108897.
PMID: 33789106
Species: Porcine
Sample Types: Whole Cells
Applications: Bioassay

Lin H et al. (2008), Adenoviral expression of vascular endothelial growth factor splice variants differentially regulate bone marrow-derived mesenchymal stem cells. J. Cell. Physiol., 216(2):458-68.
PMID: 18288639
Species: Mouse
Sample Types: In Vivo
Applications: In Vivo

Horst AK et al. (2006), Carcinoembryonic antigen-related cell adhesion molecule 1 modulates vascular remodeling in vitro and in vivo. J. Clin. Invest., 116(6):1596-605.
PMID: 16680193
Species: Mouse
Sample Types: In Vivo
Applications: In Vivo

Niida S et al. (2005), VEGF receptor 1 signaling is essential for osteoclast development and bone marrow formation in colony-stimulating factor 1-deficient mice. Proc. Natl. Acad. Sci. U.S.A., 102(39):14016-21.
PMID: 16172397
Species: Mouse
Sample Types: In Vivo
Applications: In Vivo

Qian DZ et al. (2004), The histone deacetylase inhibitor NVP-LAQ824 inhibits angiogenesis and has a greater antitumor effect in combination with the vascular endothelial growth factor receptor tyrosine kinase inhibitor PTK787/ZK222584. Cancer Res., 64(18):6626-34.
PMID: 15374977
Species: Mouse
Sample Types: In Vivo
Applications: In Vivo

Usui T et al. (2004), VEGF164(165) as the pathological isoform: differential leukocyte and endothelial responses through VEGFR1 and VEGFR2. Invest. Ophthalmol. Vis. Sci., 45(2):368-74.
PMID: 14744874
Species: Mouse
Sample Types: In Vivo
Applications: In Vivo

Banerjee K et al. (2004), CXCR2-/- mice show enhanced susceptibility to herpetic stromal keratitis: a role for IL-6-induced neovascularization. J. Immunol., 172(2):1237-45.
PMID: 14707102

Recombinant Mouse VEGF 120 Protein

R&D Systems, Inc. a Bio-Techne Brand

Key Product Details

Customers also Viewed

Product Specifications for Recombinant Mouse VEGF 120 Protein

Formulation, Preparation and Storage

Carrier Free

494-VE

Carrier

494-VE/CF

Carrier Free

Reconstitution Calculator

Background: VEGF

Citations for Recombinant Mouse VEGF 120 Protein (8)

Product Documents for Recombinant Mouse VEGF 120 Protein

Product Datasheets

COA

Certificate of Analysis

SDS

Bio-Techne Brands

Stay Connected