Recombinant Mouse Vasorin/SLIT-like 2 Protein, CF

Name: Recombinant Mouse Vasorin/SLIT-like 2 Protein, CF
Brand: R&D Systems
SKU: 8948-VN

R&D Systems, Inc. a Bio-Techne Brand

Catalog # 8948-VN

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Key Product Details

Source:	NS0
Accession #:	Q9CZT5
Applications:	Bioactivity

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Product Specifications for Recombinant Mouse Vasorin/SLIT-like 2 Protein, CF

Source	Mouse myeloma cell line, NS0-derived mouse Vasorin/SLIT-like 2 protein Cys25-Pro576, with a C-terminal 6-His tag
Purity	>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Endotoxin level	<0.10 EU per 1 μg of the protein by the LAL method.
N-terminal sequence Analysis	Cys25
Predicted Molecular Mass	61 kDa
SDS-PAGE	72-97 kDa, reducing conditions
Activity	Measured by its binding ability in a functional ELISA. When Recombinant Mouse TGF- beta 2 (Catalog # 7346-B2) is immobilized at 1 µg/mL (100 µL/well), the concentration of Recombinant Mouse Vasorin/SLIT-like 2 that produces 50% of the optimal binding response is approximately 0.3-1.8 μg/mL

Formulation, Preparation and Storage

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

8948-VN

Formulation

Lyophilized from a 0.2 μm filtered solution in MOPS, NaCl, Ethylene Glycol and Trehalose.

Reconstitution

Reconstitute at 250 μg/mL in water.

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage:

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Background: Vasorin/SLIT-like 2

Vasorin, also known as SLIT-like 2, is an approximately 110 kDa transmembrane protein that is expressed on vascular smooth muscle cells and in the developing skeletal system (1, 2). It is down-regulated following vascular injury (1). A 95 kDa soluble form of Vasorin can be shed from the cell surface by ADAM17 mediated cleavage (3). The shed soluble form of Vasorin binds to TGF-beta 1, 2, and 3 and prevents TGF-beta induced effects including intimal formation and epithelial mesenchymal transition (1, 3). Soluble Vasorin is elevated in the serum of hepatic carcinoma patients relative to normal or non-cancerous hepatic disease (4). It promotes tumor cell proliferation, migration, and survival (4). Mature mouse Vasorin consists of a 552 amino acid (aa) extracellular domain (ECD) with 10 leucine rich repeats (LRR) flanked by one LRRNT and one LRRCT domain, one EGF-like domain, and one Fibronectin type-III domain, a 21 aa transmembrane segment, and a 76 aa cytoplasmic domain (1). Within the ECD, mouse Vasorin shares 85% and 96% aa sequence identity with human and rat Vasorin, respectively.

Item	Value
References	Ikeda, Y. et al. (2004) Proc. Natl. Acad. Sci. USA 101:10732. Krautzberger, A.M. et al. (2012) Gene Expr. Patterns 12:167. Malapeira, J. et al. (2011) Oncogene 30:1912. Li, S. et al. (2015) Oncotarget 6:10045.
Entrez Gene IDs	114990 (Human); 246154 (Mouse)
Alternate Names	Atia, Protein slit-like 2, SLIT like 2, Slitl2, VASN, Vasorin, slit-like 2, slit-like 2 (Drosophila)

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Recombinant Mouse Vasorin/SLIT-like 2 Protein, CF

R&D Systems, Inc. a Bio-Techne Brand

Key Product Details

Complete Your Research

Complete Your Experiment

Product Specifications for Recombinant Mouse Vasorin/SLIT-like 2 Protein, CF

Formulation, Preparation and Storage

Carrier Free

Reconstitution Calculator

Background: Vasorin/SLIT-like 2

Citations for Recombinant Mouse Vasorin/SLIT-like 2 Protein, CF

Product Documents for Recombinant Mouse Vasorin/SLIT-like 2 Protein, CF

Product Datasheets

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