Product Specifications for Recombinant Mouse Vasorin/SLIT-like 2 Protein, CF
Mouse myeloma cell line, NS0-derived mouse Vasorin/SLIT-like 2 protein Cys25-Pro576, with a C-terminal 6-His tag
>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
<0.10 EU per 1 μg of the protein by the LAL method.
N-terminal sequence Analysis
Predicted Molecular Mass
72-97 kDa, reducing conditions
Measured by its binding ability in a functional ELISA. When Recombinant Mouse TGF- beta 2 (Catalog # 7346-B2) is immobilized at 1 µg/mL (100 µL/well), the concentration of Recombinant Mouse Vasorin/SLIT-like 2 that produces 50% of the optimal binding response is approximately 0.3-1.8 μg/mL
Formulation, Preparation and Storage
What does CF mean?
CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our
Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant
protein to be stored at a more dilute concentration.
The carrier free version does not contain BSA.
What formulation is right for me?
In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or
as an ELISA standard.
In contrast, the carrier free protein is recommended for applications, in which the presence of BSA
Lyophilized from a 0.2 μm filtered solution in MOPS, NaCl, Ethylene Glycol and Trehalose.
Reconstitute at 250 μg/mL in water.
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage:
Background: Vasorin/SLIT-like 2
Vasorin, also known as SLIT-like 2, is an approximately 110 kDa transmembrane protein that is expressed on vascular smooth muscle cells and in the developing skeletal system (1, 2). It is down-regulated following vascular injury (1). A 95 kDa soluble form of Vasorin can be shed from the cell surface by ADAM17 mediated cleavage (3). The shed soluble form of Vasorin binds to TGF-beta 1, 2, and 3 and prevents TGF-beta induced effects including intimal formation and epithelial mesenchymal transition (1, 3). Soluble Vasorin is elevated in the serum of hepatic carcinoma patients relative to normal or non-cancerous hepatic disease (4). It promotes tumor cell proliferation, migration, and survival (4). Mature mouse Vasorin consists of a 552 amino acid (aa) extracellular domain (ECD) with 10 leucine rich repeats (LRR) flanked by one LRRNT and one LRRCT domain, one EGF-like domain, and one Fibronectin type-III domain, a 21 aa transmembrane segment, and a 76 aa cytoplasmic domain (1). Within the ECD, mouse Vasorin shares 85% and 96% aa sequence identity with human and rat Vasorin, respectively.
Ikeda, Y. et al. (2004) Proc. Natl. Acad. Sci. USA 101:10732.
Krautzberger, A.M. et al. (2012) Gene Expr. Patterns 12:167.
Malapeira, J. et al. (2011) Oncogene 30:1912.
Li, S. et al. (2015) Oncotarget 6:10045.
Entrez Gene IDs
114990 (Human); 246154 (Mouse)
Atia, Protein slit-like 2, SLIT like 2, Slitl2, VASN, Vasorin, slit-like 2, slit-like 2 (Drosophila)
Citations for Recombinant Mouse Vasorin/SLIT-like 2 Protein, CF
Please visit our Instrument Citation Database to search our collection of scientific articles that feature our instruments.
Product Documents for Recombinant Mouse Vasorin/SLIT-like 2 Protein, CF
Which Brands are Currently Available on bio‑techne.com? R&D Systems, Tocris Bioscience and ProteinSimple branded products are available to purchase through bio‑techne.com. ProteinSimple branded instruments are available to quote. ACD branded products will be available on bio‑techne.com in the near future. Novus Biologicals branded products are not currently available on bio‑techne.com and can be found at novusbio.com.
Setting the standard in quality research reagents for over 30 years
A trusted leader in quality life science reagents
Your trusted supplier for innovative and high performance life science reagents
Proprietary systems and consumables for simpler, more quantitative and affordable protein analysis
Proprietary RNAscope® technology capable of detecting and quantifying RNA biomarkers in situ at single molecule sensitivity
A world leader in developing liquid biopsy based diagnostics