Recombinant Mouse u-Plasminogen Activator (uPA) Protein, CF

Name: Recombinant Mouse u-Plasminogen Activator (uPA) Protein, CF
Brand: R&D Systems
SKU: 11143-SE

R&D Systems, Inc. a Bio-Techne Brand

Catalog # 11143-SE

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/Urokinase His-tag

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Key Product Details

Source:	NS0
Accession #:	NP_032899.1
Applications:	Enzyme Activity

View Full Technical Details for Recombinant Mouse u-Plasminogen Activator (uPA) Protein, CF

Product Specifications for Recombinant Mouse u-Plasminogen Activator (uPA) Protein, CF

Source	Mouse myeloma cell line, NS0-derived mouse u-Plasminogen Activator (uPA)/Urokinase protein Gly21-Phe433, with a C-terminal 6-His tag
Purity	>90%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Endotoxin level	<0.10 EU per 1 μg of the protein by the LAL method.
N-terminal sequence Analysis	Lys157, Ile180, Met361
Predicted Molecular Mass	18 kDa (long A chain), 3 kDa (short A chain), 29 kDa (B chain)
SDS-PAGE	24-33 kDa (short form), under non-reducing conditions
Activity	Measured by its ability to cleave a peptide substrate, N-carbobenzyloxy-Gly-Gly-Arg-7-amido-4-methylcoumarin (Z-GGR-AMC). The specific activity is >1700 pmol/min/μg, as measured under the described conditions.

Scientific Data Examples for Recombinant Mouse u-Plasminogen Activator (uPA) Protein, CF

	Recombinant Mouse u‑Plasminogen Activator (uPA)/Urokinase His-tag Protein Enzyme Activity. Recombinant Mouse u-Plasminogen Activator (uPA)/Urokinase His-tag (Catalog # 11143-SE) is measured by its ability to cleave a peptide substrate, N-carbobenzyloxy-Gly-Gly-Arg-7-amido-4-methylcoumarin (Z-GGR-AMC).

Formulation, Preparation and Storage

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

11143-SE

Formulation	Supplied as a 0.2 μm filtered solution in HEPES, NaCl and CaCl₂.
Shipping	The product is shipped with dry ice or equivalent. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage:	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 6 months from date of receipt, -20 to -70 °C as supplied. 3 months, -20 to -70 °C under sterile conditions after opening.

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Background: u-Plasminogen Activator (uPA)/Urokinase

Urokinase Plasminogen Activator (uPA), also known as u-plasminogen activator or urokinase, is a highly-specific serine protease from the peptidase S1 family that cleaves plasminogen to form plasmin making it a key player in the plasminogen activator (PA) system (1, 2). In cancer, the PA system plays a commanding role in tumor growth, angiogenesis, tumor cell invasion, migration, and metastasis. Expression of uPA is minimal in normal cells but is increased several fold in tumor cells by extracellular stimuli elevated in cancer (3) and corresponds to poor outcomes in several types of cancer (2, 4-7). Therefore, uPA has been identified as an excellent target for therapeutic development through inhibition of protease activity or though inhibition of uPA-dependent signaling while in complex with uPA receptor (uPAR) (2, 7). The pro-enzyme of uPA is synthesized with an N-terminal signal peptide and processed into an active disulfide-linked two-chain molecule (2, 7-10). For mouse uPA, the B chain starting at Ile180 corresponds to the catalytic domain. Two forms of the A chain exist, one starting at Gly21 (the long form) and the other at Lys157 (the short form). While the B chain is common for both forms, the long and short A chains are unique to expected 47 kDa and 32 kDa two-chain forms, respectively. The long A chain contains an EGF-like domain and the kringle domain. The long A domain is reported as responsible for the binding of the uPA receptor (uPAR) (2, 7).

Item	Value
References	Ellis, V. (2004) in Handbook of Proteolytic Enzymes. Barrett, A.J. et al. eds., Academic Press, San Diego, pp.1677. Mahmood, N. et al. (2018) Front Oncol. 8:24. Nagamine, Y. et al. (2005) Thromb. Haemost. 93:661. Duffy, M. and C. Duggan. (2004) Clin. Biochem. 37:541. Pappot, H. et al. (2006) Lung Cancer 51:193. Taubert, H. et al. (2010) Br. J. Cancer 102:731. Masucci, M.T. et al. (2022) Cancers. 14:498. Riccio, A. et al. (1985) Nucleic Acids Res. 13:2759. Nagai, M. et al. (1985) Gene 36:183. Jacobs, P. et al. (1985) DNA 4:139.
Long Name	Urokinase-type Plasminogen Activator
Entrez Gene IDs	5328 (Human); 18792 (Mouse); 102135886 (Cynomolgus Monkey)
Alternate Names	ATF, EC 3.4.21, EC 3.4.21.73, PLAU, Urokinase, plasminogen activator, urokinase, u-PA, u-Plasminogen Activator, uPA, uPlasminogen Activator, urinary, urokinase-type plasminogen activator

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Recombinant Mouse u-Plasminogen Activator (uPA) Protein, CF

R&D Systems, Inc. a Bio-Techne Brand

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Product Specifications for Recombinant Mouse u-Plasminogen Activator (uPA) Protein, CF

Scientific Data Examples for Recombinant Mouse u-Plasminogen Activator (uPA) Protein, CF

Formulation, Preparation and Storage

Carrier Free

Reconstitution Calculator

Background: u-Plasminogen Activator (uPA)/Urokinase

Citations for Recombinant Mouse u-Plasminogen Activator (uPA) Protein, CF

Product Documents for Recombinant Mouse u-Plasminogen Activator (uPA) Protein, CF

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