Recombinant Mouse Tryptase beta-1/Mcpt7 Protein, CF
R&D Systems, part of Bio-Techne | Catalog # 1937-SE
Key Product Details
- R&D Systems NS0-derived Recombinant Mouse Tryptase beta-1/Mcpt7 Protein (1937-SE)
- Quality control testing to verify active proteins with lot specific assays by in-house scientists
- All R&D Systems proteins are covered with a 100% guarantee
Source
NS0
Accession #
Structure / Form
Mature form
Conjugate
Unconjugated
Applications
Enzyme Activity
Product Specifications
Source
Mouse myeloma cell line, NS0-derived mouse Tryptase beta-1/MCP-7 protein
Ile29-Phe273, with an C-terminal 10-His tag
Ile29-Phe273, with an C-terminal 10-His tag
Purity
>95%, by SDS-PAGE under reducing conditions and visualized by silver stain.
Endotoxin Level
<1.0 EU per 1 μg of the protein by the LAL method.
N-terminal Sequence Analysis
Ile29
Predicted Molecular Mass
28.8 kDa
SDS-PAGE
36 kDa and 37 kDa, reducing conditions
Activity
Measured by its ability to cleave a colorimetric peptide substrate, N-carbobenzyloxy-Lys-ThioBenzyl ester (Z-Lys-SBzl), in the presence of 5,5’Dithio-bis (2-nitrobenzoic acid) (DTNB). Edwards, K.M. et al. (1999) J. Biol. Chem. 274:30468.
The specific activity is >75 pmol/min/µg, as measured under the described conditions.
The specific activity is >75 pmol/min/µg, as measured under the described conditions.
Formulation, Preparation and Storage
1937-SE
| Formulation | Supplied as a 0.2 μm filtered solution in Tris, NaCl and CaCl2. |
| Shipping | The product is shipped with dry ice or equivalent. Upon receipt, store it immediately at the temperature recommended below. |
| Stability & Storage | Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
|
Background: Tryptase beta-1/MCP-7
Tryptase beta-1 is a serine protease with trypsin-like activity, which is sometimes also referred to as Mast Cell Protease 7 (1). It is stored in the secretory granules of mouse mast cells (2). It exhibits anticoagulant activity due to its ability to degrade fibrinogen in the presence of the diverse array of protease inhibitors in plasma (3). The deduced amino acid sequence for mouse Tryptase beta-1 consists of a signal peptide (residues 1-18), a pro region (residues 19-28) and the mature chain (residue 29‑273). The mature chain was expressed and purified.
References
- McNeil, H.P. et al. (1992) Proc. Natl. Acad. Sci. USA 89:11174.
- Matsumoto, R et al. (1995) J. Biol. Chem. 270:19524.
- Huang, C. et al. (1997) J. Biol. Chem. 272:31885.
Long Name
Mast Cell Protease 7
Alternate Names
Mast Cell Tryptase, MCP-7, Mcp7, Mcpt7, TPSB1, TRYB1, Tryptase beta1
Entrez Gene IDs
100503895 (Mouse)
Gene Symbol
TPSAB1
UniProt
Additional Tryptase beta-1/MCP-7 Products
Product Documents for Recombinant Mouse Tryptase beta-1/Mcpt7 Protein, CF
Product Specific Notices for Recombinant Mouse Tryptase beta-1/Mcpt7 Protein, CF
For research use only
Loading...
Loading...
Loading...
Loading...