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Recombinant Mouse TRANCE/RANK L/TNFSF11 (E. coli-expressed) Best Seller

Catalog # 462-TEC | R&D Systems, Inc. a Bio-Techne Brand
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Carrier Free
462-TEC-010/CF

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With Carrier
462-TEC-010

Key Product Details

Source

E. coli

Accession #

Conjugate

Unconjugated

Applications

Bioactivity

Product Specifications

Source

E. coli-derived mouse TRANCE/TNFSF11/RANK L protein
Lys158-Asp316, with an N-terminal Met

Purity

>95%, by SDS-PAGE under reducing conditions and visualized by silver stain.

Endotoxin Level

<0.01 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

Met

Predicted Molecular Mass

19 kDa

Activity

Measured by its ability to induce osteoclast differentiation of RAW 264.7 mouse monocyte/macrophage cells.
The ED50 for this effect is 0.5‑2 ng/mL.

Scientific Data Images for Recombinant Mouse TRANCE/RANK L/TNFSF11 (E. coli-expressed)

Recombinant Mouse TRANCE/RANK L/TNFSF11 (E. coli-expressed) Bioactivity

Recombinant Mouse TRANCE/RANK L/TNFSF11 (E. coli-expressed) Bioactivity

Recombinant Mouse TRANCE/TNFSF11/RANK L (Catalog # 462-TEC) induces osteoclast differentiation of the RAW 264.7 mouse monocyte/macrophage cell line. The ED50 for this effect is 0.5-2 ng/mL.
Recombinant Mouse TRANCE/RANK L/TNFSF11 (E. coli-expressed) SDS-PAGE

Recombinant Mouse TRANCE/RANK L/TNFSF11 (E. coli-expressed) SDS-PAGE

1 μg/lane of Recombinant Mouse TRANCE/TNFSF11/RANK L was resolved with SDS-PAGE under reducing (R) conditions and visualized by silver staining, showing a single band at 19 kDa.

Formulation, Preparation and Storage

Carrier Free
What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

Carrier: 462-TEC
Formulation Lyophilized from a 0.2 μm filtered solution in NaH2PO4, NaCl and EDTA with BSA as a carrier protein.
Reconstitution Reconstitute at 100 μg/mL in sterile PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Carrier Free: 462-TEC/CF
Formulation Lyophilized from a 0.2 μm filtered solution in NaH2PO4, NaCl and EDTA.
Reconstitution Reconstitute at 100 μg/mL in sterile PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: TRANCE/TNFSF11/RANK L

TRANCE (receptor activator of NF-kappa B ligand [RANK L], also called TNF-related activation-induced cytokine [TRANCE], osteoprotegrin ligand [OPGL], and osteoclast differentiation factor [ODF]), is a member of the tumor necrosis factor (TNF) family. TRANCE was originally identified as an immediate early gene up-regulated by T cell receptor stimulation. The mouse TRANCE cDNA encodes a type II transmembrane protein of 316 amino acids with a predicted cytoplasmic domain of 48 amino acids and an extracellular domain of 247 amino acids. The extracellular domain contains two potential N-linked glycosylation sites. Mouse and human TRANCE share 85% amino acid identity. TRANCE is primarily expressed in T cells and T cell rich organs, such as thymus and lymph nodes. The multi-functions of TRANCE include induction of activation of the c-jun N-terminal kinase, enhancement of T cell growth and dendritic cell function, induction of osteoclastogenesis, and lymph node organogenesis. RANK is the cell surface signaling receptor of TRANCE. RANK has been shown to undergo receptor clustering during signal transduction. Osteoprotegrin, a soluble member of the TNF receptor family which binds TRANCE, is a naturally occurring decoy receptor that counterbalances the effects of TRANCE.

References

  1. Wong, B.R. et al. (1997) J. Biol. Chem. 272:25190.
  2. Anderson, D.M. et al. (1997) Nature 390:175.
  3. Nakagawa, N. et al. (1998) Biochem. Biophys. Res. Commun. 245:382.
  4. Kong, Y-Y. et al. (1999) Nature 397:315.

Long Name

TNF-related Activation-induced Cytokine

Alternate Names

CD254, ODF, OPGL, RANK L, RANKL, TNFSF11

Entrez Gene IDs

8600 (Human); 21943 (Mouse); 117516 (Rat); 102137946 (Cynomolgus Monkey)

Gene Symbol

TNFSF11

UniProt

Product Documents for Recombinant Mouse TRANCE/RANK L/TNFSF11 (E. coli-expressed)

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Note: Certificate of Analysis not available for kit components.

Product Specific Notices for Recombinant Mouse TRANCE/RANK L/TNFSF11 (E. coli-expressed)

For research use only

Citations for Recombinant Mouse TRANCE/RANK L/TNFSF11 (E. coli-expressed) (82)

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Customer Reviews for Recombinant Mouse TRANCE/RANK L/TNFSF11 (E. coli-expressed) (14)

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  • Recombinant Mouse TRANCE/RANK L/TNFSF11 (E. coli-expressed)
    Name: Anonymous
    Application: In vitro bioactivity in cell culture
    Verified Customer | Posted 04/21/2022
    Recombinant Mouse TRANCE/RANK L/TNFSF11 (E. coli-expressed) 462-TEC
  • Recombinant Mouse TRANCE/RANK L/TNFSF11 (E. coli-expressed)
    Name: Anonymous
    Application: In vitro bioactivity in cell culture
    Verified Customer | Posted 12/31/2021
    Recombinant Mouse TRANCE/RANK L/TNFSF11 (E. coli-expressed) 462-TEC
  • Name: Anonymous
    Application: IHC
    Verified Customer | Posted 11/23/2021
    Recombinant Mouse TRANCE/RANK L/TNFSF11 (E. coli-expressed) 462-TEC
  • Recombinant Mouse TRANCE/RANK L/TNFSF11 (E. coli-expressed)
    Name: Anonymous
    Application: Stem/Immune cell maintenance or differentiation
    Verified Customer | Posted 11/04/2021
    Recombinant Mouse TRANCE/RANK L/TNFSF11 (E. coli-expressed) 462-TEC
  • Recombinant Mouse TRANCE/RANK L/TNFSF11 (E. coli-expressed)
    Name: Anonymous
    Application: In vitro bioactivity in cell culture
    Verified Customer | Posted 03/20/2021
    Recombinant Mouse TRANCE/RANK L/TNFSF11 (E. coli-expressed) 462-TEC
  • Recombinant Mouse TRANCE/RANK L/TNFSF11 (E. coli-expressed)
    Name: Anonymous
    Application: Cell Proliferation
    Verified Customer | Posted 03/11/2021
    Recombinant Mouse TRANCE/RANK L/TNFSF11 (E. coli-expressed) 462-TEC
  • Recombinant Mouse TRANCE/RANK L/TNFSF11 (E. coli-expressed)
    Name: Anonymous
    Application: In vitro bioactivity in cell culture
    Verified Customer | Posted 02/18/2021
    Recombinant Mouse TRANCE/RANK L/TNFSF11 (E. coli-expressed) 462-TEC
  • Recombinant Mouse TRANCE/RANK L/TNFSF11 (E. coli-expressed)
    Name: Anonymous
    Application: In vitro bioactivity in cell culture
    Verified Customer | Posted 10/25/2020
    Recombinant Mouse TRANCE/RANK L/TNFSF11 (E. coli-expressed) 462-TEC
  • Recombinant Mouse TRANCE/RANK L/TNFSF11 (E. coli-expressed)
    Name: Anonymous
    Application: In vitro bioactivity in cell culture
    Verified Customer | Posted 06/25/2020
    Recombinant Mouse TRANCE/RANK L/TNFSF11 (E. coli-expressed) 462-TEC
  • Recombinant Mouse TRANCE/RANK L/TNFSF11 (E. coli-expressed)
    Name: Anonymous
    Application: cell differentiation
    Verified Customer | Posted 02/21/2020
    Recombinant Mouse TRANCE/RANK L/TNFSF11 (E. coli-expressed) 462-TEC
  • Recombinant Mouse TRANCE/RANK L/TNFSF11 (E. coli-expressed)
    Name: Anonymous
    Application: MassSpec characterization.
    Verified Customer | Posted 01/08/2019
    Recombinant Mouse TRANCE/RANK L/TNFSF11 (E. coli-expressed) 462-TEC
  • Recombinant Mouse TRANCE/RANK L/TNFSF11 (E. coli-expressed)
    Name: Joseph Moore
    Application: Cell Adhesion
    Verified Customer | Posted 01/03/2019
    Bone marrow macrophages grown in media on osteoassay plates for 7 days then treated with 35ng/mL Rank L. After 14 days TRAP stained.
  • Recombinant Mouse TRANCE/RANK L/TNFSF11 (E. coli-expressed)
    Name: Beata Lecka-Czernik
    Application: Media additive for protein or antibody production
    Verified Customer | Posted 05/16/2016
  • Recombinant Mouse TRANCE/RANK L/TNFSF11 (E. coli-expressed)
    Name: Beata Lecka-Czernik
    Application: Stem/Immune cell maintenance or differentiation
    Verified Customer | Posted 01/18/2016

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