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Recombinant Mouse TNFRH3/TNFRSF26 Fc Chimera Protein, CF

R&D Systems, part of Bio-Techne | Catalog # 5330-RH

R&D Systems, part of Bio-Techne
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5330-RH-050

Key Product Details

Source

CHO

Accession #

Structure / Form

Disulfide-linked homodimer

Conjugate

Unconjugated

Applications

Bioactivity

Product Specifications

Source

Chinese Hamster Ovary cell line, CHO-derived mouse TNFRH3/TNFRSF26 protein
Mouse TNFRH3
(Ser20-Lys162)
Accession # P83626
IEGRMDP Mouse IgG2A
(Glu98-Lys330)
N-terminus C-terminus

Purity

>95%, by SDS-PAGE under reducing conditions and visualized by silver stain.

Endotoxin Level

<0.01 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

Ser20

Predicted Molecular Mass

43.2 kDa (monomer)

SDS-PAGE

60-65 kDa, reducing conditions

Activity

Measured by its binding ability in a functional ELISA.
When Recombinant Mouse TNFRH3/TNFRSF26 Fc Chimera is immobilized at 2 μg/mL (100 μL/well), the concentration of Recombinant Mouse TRAIL/TNFSF10 (Catalog # 1121-TL) that produces 50% of the optimal binding response is approximately 15-75 ng/mL.

Formulation, Preparation and Storage

5330-RH
Formulation Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.
Reconstitution
Reconstitute at 100 μg/mL in PBS.

Reconstitution Buffer Available:
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Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: TNFRH3/TNFRSF26

Mouse TNFRH3 (Tumor necrosis factor receptor homolog 3; designated TNFR superfamily member 26) is a member of the TNF receptor superfamily. Northern blot analysis indicates its expression in the mouse spleen and thymus, and by a variety of mouse lymphoid cell lines (1). Mature mouse TNFRH3 is a 21 kDa (predicted, unglycosylated), 185 amino acid (aa) type I transmembrane protein that contains a 145 aa extracellular domain (ECD) and a 19 aa cytoplasmic domain. The ECD contains two potential sites of N‑linked glycosylation and three TNFR-Cys repeats that characterize TNF receptors, while the cytoplasmic portion lacks a death domain (1). No ortholog to mouse TNFRH3 has been reported in human or rat (1). Two TNFRH3‑related mouse TNF receptor homologs, DcTRAIL R1 (TNFRSF23/TNFRH1, 35% aa sequence identity within the ECD) and DcTRAIL R2 (TNFRSF22/TNFRH2, 40% aa sequence identity within the ECD) are classed as TRAIL decoy receptors. One report indicates that TNFRH3 binding of transfected TRAIL/TNFSF10 is below limits of detection in a cell-based assay, and it is thus thought not to serve as a TRAIL decoy receptor (1). Similarly, binding was not detected to any of 38 human and mouse TNF superfamily members in a cell‑based assay (2). However, TNFRH3 does bind recombinant trimeric mouse TRAIL in R&D Systems’ direct ELISA assay.

References

  1. Schneider, P. et al. (2003) J. Biol. Chem. 278:5444.
  2. Bossen, C. et al. (2006) J. Biol. Chem. 281:13964.

Long Name

Tumor Necrosis Factor Receptor Homolog 3

Alternate Names

Tnfrsf24, Tnfrsf26

Entrez Gene IDs

244237 (Mouse)

Gene Symbol

TNFRSF26

UniProt

Additional TNFRH3/TNFRSF26 Products

Product Documents for Recombinant Mouse TNFRH3/TNFRSF26 Fc Chimera Protein, CF

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Note: Certificate of Analysis not available for kit components.

Product Specific Notices for Recombinant Mouse TNFRH3/TNFRSF26 Fc Chimera Protein, CF

For research use only

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