Recombinant Mouse TNFRH3/TNFRSF26 Fc Chimera Protein, CF
R&D Systems, part of Bio-Techne | Catalog # 5330-RH
Key Product Details
Source
Accession #
Structure / Form
Conjugate
Applications
Product Specifications
Source
Mouse TNFRH3 (Ser20-Lys162) Accession # P83626 |
IEGRMDP | Mouse IgG2A (Glu98-Lys330) |
N-terminus | C-terminus |
Purity
Endotoxin Level
N-terminal Sequence Analysis
Predicted Molecular Mass
SDS-PAGE
Activity
When Recombinant Mouse TNFRH3/TNFRSF26 Fc Chimera is immobilized at 2 μg/mL (100 μL/well), the concentration of Recombinant Mouse TRAIL/TNFSF10 (Catalog # 1121-TL) that produces 50% of the optimal binding response is approximately 15-75 ng/mL.
Formulation, Preparation and Storage
5330-RH
Formulation | Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. |
Reconstitution |
Reconstitute at 100 μg/mL in PBS.
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Shipping | The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. |
Stability & Storage | Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
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Background: TNFRH3/TNFRSF26
Mouse TNFRH3 (Tumor necrosis factor receptor homolog 3; designated TNFR superfamily member 26) is a member of the TNF receptor superfamily. Northern blot analysis indicates its expression in the mouse spleen and thymus, and by a variety of mouse lymphoid cell lines (1). Mature mouse TNFRH3 is a 21 kDa (predicted, unglycosylated), 185 amino acid (aa) type I transmembrane protein that contains a 145 aa extracellular domain (ECD) and a 19 aa cytoplasmic domain. The ECD contains two potential sites of N‑linked glycosylation and three TNFR-Cys repeats that characterize TNF receptors, while the cytoplasmic portion lacks a death domain (1). No ortholog to mouse TNFRH3 has been reported in human or rat (1). Two TNFRH3‑related mouse TNF receptor homologs, DcTRAIL R1 (TNFRSF23/TNFRH1, 35% aa sequence identity within the ECD) and DcTRAIL R2 (TNFRSF22/TNFRH2, 40% aa sequence identity within the ECD) are classed as TRAIL decoy receptors. One report indicates that TNFRH3 binding of transfected TRAIL/TNFSF10 is below limits of detection in a cell-based assay, and it is thus thought not to serve as a TRAIL decoy receptor (1). Similarly, binding was not detected to any of 38 human and mouse TNF superfamily members in a cell‑based assay (2). However, TNFRH3 does bind recombinant trimeric mouse TRAIL in R&D Systems’ direct ELISA assay.
References
- Schneider, P. et al. (2003) J. Biol. Chem. 278:5444.
- Bossen, C. et al. (2006) J. Biol. Chem. 281:13964.
Long Name
Alternate Names
Entrez Gene IDs
Gene Symbol
UniProt
Additional TNFRH3/TNFRSF26 Products
Product Documents for Recombinant Mouse TNFRH3/TNFRSF26 Fc Chimera Protein, CF
Product Specific Notices for Recombinant Mouse TNFRH3/TNFRSF26 Fc Chimera Protein, CF
For research use only