Recombinant Mouse TRAIL/TNFSF10 Protein
Catalog # 1121-TL | R&D Systems, Inc. a Bio-Techne Brand
Key Product Details
Product Specifications
Source
Pro118-Asn291
Purity
Endotoxin Level
N-terminal Sequence Analysis
Predicted Molecular Mass
Activity
The ED50 for this effect is 0.2-1.2 ng/mL.
Formulation, Preparation and Storage
Carrier Free
What does CF mean?CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.
What formulation is right for me?In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.
Carrier: 1121-TL
Formulation | Lyophilized from a 0.2 μm filtered solution in Tris, Ammonium Sulfate and ZnSO4 with Trehalose and with BSA as a carrier protein. |
Reconstitution | Reconstitute at 200 μg/mL in sterile PBS. |
Shipping | The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. |
Stability & Storage | Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
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Carrier Free: 1121-TL/CF
Formulation | Supplied as a 0.2 μm filtered solution in Tris, Ammonium Sulfate and ZnSO4. |
Shipping | The product is shipped with dry ice or equivalent. Upon receipt, store it immediately at the temperature recommended below. |
Stability & Storage | Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
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Background: TRAIL/TNFSF10
TNF-related apoptosis-inducing ligand (TRAIL), also called apoptosis 2 ligand (Apo2L) for its similarity in sequence, structure, and function to Fas Ligand/Apo1L, is a 33-35 kDa type II transmembrane glycoprotein of the tumor necrosis factor superfamily, designated TNFSF10 (1‑3). Mouse TRAIL cDNA encodes a 17 amino acid (aa) N-terminal intracellular domain, a 20 aa transmembrane domain and a 253 aa extracellular domain. Like most TNF family members, TRAIL is bioactive as a homotrimer (1). Unlike other TNF family members, a zinc ion complexed by human Cys 230 (mouse Cys 240) of each of the three monomers is critical for structural stability (4, 5). Either transmembrane or cysteine protease-released soluble sTRAIL induce apoptosis of many transformed cell lines, but rarely of normal cells (3, 6). Accordingly, TRAIL is suggested to have a role in tumor surveillance (1). Mice with genetically disrupted TRAIL have defective thymocyte apoptosis, creating faulty negative selection and some increased susceptibility to induced autoimmune diseases (7). In humans, TRAIL controls apoptosis of erythrocyte precursors and sTRAIL is inversely correlated with hemoglobin (1, 8). TRAIL transcripts are constitutively expressed in a variety of human (and presumably mouse) tissues and mononuclear cells (2, 3). Only one of two receptors that transduce apoptotic signals in humans is found in the mouse (TRAIL R2/DR5 but not TRAIL R1/DR4) (1). Mice express TRAIL receptors DcTRAIL R1/TNFRSF23 and DcTRAIL R2/TNFRSF22. These receptors lack death domains, but differ in structure from human regulatory receptors TRAIL R3 and TRAIL R4 (9). Osteoprotegerin has been identified in humans as a TRAIL receptor, but binding in mouse has not yet been demonstrated (1, 10). Mouse TRAIL shows 85% aa identity with rat TRAIL and 70% aa identity with human, bovine, and porcine TRAIL within the TNF homology domain (aa 118-291).
References
- Zauli, G. and P. Secchiero (2006) Cytokine Growth Factor Rev. 17:245.
- Wiley, S.R. et al. (1995) Immunity 3:673.
- Pitti, R.M. et al. (1996) J. Biol. Chem. 271:12687.
- Bodmer, J.L. et al. (2000) J. Biol. Chem. 275:20632.
- Hymowitz, S.G. et al. (2000) Biochemistry 39:633.
- Sedger, L. M. et al. (2002) Eur. J. Immunol. 32:2246.
- Lamhamedi-Cherradi, S.E. et al. (2003) Nat. Immunol. 4:255.
- Choi, J.W. (2005) Ann. Hematol. 84:728.
- Schneider, P. et al. (2003) J. Biol. Chem. 278:5444.
- Emery, J. et al. (1998) J. Biol. Chem. 273:14363.
Long Name
Alternate Names
Gene Symbol
UniProt
Product Documents for Recombinant Mouse TRAIL/TNFSF10 Protein
Product Specific Notices for Recombinant Mouse TRAIL/TNFSF10 Protein
For research use only
Citations for Recombinant Mouse TRAIL/TNFSF10 Protein (7)
Citations are publications that use Bio-Techne products. Selected citations for Recombinant Mouse TRAIL/TNFSF10 Protein include:
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Species: Mouse
Sample Types: In Vivo
Applications: In VivoS Tian et al. (2018-11-16), Aldehyde Dehydrogenase 1 (ALDH1) Promotes the Toxicity of TRAIL in Non-Small Cell Lung Cancer Cells via Post-Transcriptional Regulation of MEK-1 Expression Cell. Physiol. Biochem., 2018-151(1):217-227.
PMID: 30448845 -
Species: Mouse
Sample Types: Whole Cells
Applications: BioassaySu et al. (2015-02-10), Two-signal requirement for growth-promoting function of Yap in hepatocytes. Elife, 2015-04(0).
PMID: 25667983 -
Species: Mouse
Sample Types: Whole Cells
Applications: BioassayKichev A et al. (2014-02-07), Tumor necrosis factor-related apoptosis-inducing ligand (TRAIL) signaling and cell death in the immature central nervous system after hypoxia-ischemia and inflammation. J Biol Chem, 2014-0289(13):9430-9.
PMID: 24509861 -
Species: Mouse
Sample Types: In Vivo
Applications: In VivoStarkey M et al. (2013-09-18), Tumor necrosis factor-related apoptosis-inducing ligand translates neonatal respiratory infection into chronic lung disease. Mucosal Immunol, 2013-07(3):478-88.
PMID: 24045576 -
Species: Mouse
Sample Types: Whole Cells
Applications: Bioassay(2012-06-04), Regulation of innate CD8+ T-cell activation mediated by cytokines. Proc Natl Acad Sci U S A, 2012-0109(25):9971-6.
PMID: 22665806 -
Species: Mouse
Sample Types: In Vivo
Applications: In VivoKayamuro H et al. (2009-05-04), TNF superfamily member, TL1A, is a potential mucosal vaccine adjuvant. Biochem. Biophys. Res. Commun., 2009-0384(3):296-300.
PMID: 19406102 -
Species: Mouse
Sample Types: Whole Cells
Applications: BioassayDumitru CA et al. (2007-08-01), Doxorubicin enhances TRAIL-induced cell death via ceramide-enriched membrane platforms. Apoptosis, 2007-012(8):1533-41.
PMID: 17520194
There are no citations that match your criteria.
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