Skip to main content

Recombinant Mouse TLR2 Fc Chimera Protein, CF

Bio-Techne includes R&D Systems | Catalog # 1530-TR

Catalog #
Availability
Size / Price
Qty
Loading...
1530-TR-050

Key Product Details

Source

Sf 21 (baculovirus)

Accession #

Structure / Form

Disulfide-linked homodimer

Conjugate

Unconjugated

Applications

Binding Activity

Product Specifications

Source

Spodoptera frugiperda, Sf 21 (baculovirus)-derived mouse TLR2 protein
Mouse TLR2
(Gln25-Leu590)
Accession # Q9QUN7
DIEGRMD Human IgG1
(Pro100-Lys330)
N-terminus C-terminus

Purity

>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

No results obtained: Gln25 predicted

Predicted Molecular Mass

90.4 kDa (monomer)

SDS-PAGE

92 kDa, reducing conditions

Activity

Measured by its binding ability in a functional ELISA.
Immobilized peptidoglycan at 10 µg/mL (100 µL/well) can bind recombinant mouse TLR2 Fc Chimera with a linear range of 0.04-30 µg/mL.

Formulation, Preparation and Storage

1530-TR
Formulation Lyophilized from a 0.2 μm filtered solution in PBS, NaCl and PEG 3350.
Reconstitution
Reconstitute at 100 μg/mL in sterile PBS.

Reconstitution Buffer Available:
Size / Price
Qty
Loading...
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: TLR2

The Toll-like family of molecules are a group of integral membrane proteins that serve as pattern recognition receptors for microbial pathogens (1 - 4). To date, there are at least eleven mouse and ten human members that activate the innate immune system following exposure to a variety of microbial species (1, 3). All Toll-like receptors (TLRs) are type I transmembrane (TM) proteins that exist either in the plasma membrane or in the membranes of endosomal structures (where they bind intracellular nucleic acids) (3). All TLRs also contain a large number of extracellular leucine-rich repeats (LRRs) and a cytoplasmic tail with a Toll/IL-1 receptor (TIR) domain. Mouse Toll-like receptor-2 (TLR2) is a 97 kDa, 760 amino acid (aa) glycoprotein that contains a 563 aa extracellular region, a 21 aa TM segment, and a 176 aa cytoplasmic domain (5, 6). The extracellular region contains 16 leucine-rich repeats, while the cytoplasmic tail shows one 146 aa TIR domain. The receptor is expressed on a number of cell types including T cells ( alpha beta and gamma delta), monocytes, dendritic cells, neutrophils, B cells, endothelial cells, mast cells, NK cells, macrophages and hepatocytes (1, 4, 5, 7, 8). TLR2 functions as part of a heterodimeric complex with either TLR1 or TLR6 (1, 3, 4). These complexes recognize lipoproteins and glycolipids from gram-positive and gram-negative bacteria as well as mycoplasma and yeast. TLR2/TLR1 heterodimers recognize triacylated lipopeptides from a variety of microorganisms. The TLR2/TLR6 heterodimer preferentially recognizes diacylated lipopeptides (9). Biglycan is also known to activate TLR2, but the context is unclear (8). Notably, in human, TLR2 also dimerizes with TLR10. But the TLR10 gene in mouse (but not rat) is mutationally inactive, and thus this complex is nonfunctional (10). Upon ligand recognition, TLR2 delivers an activating signal via the associated adapter molecules, MyD88 and TIRAP (1, 11). Activation via TLR2 also results in production of a number of pro-inflammatory cytokines including TNF-alpha, IL-2, IL-6, IL-12, and MIP-2 (1, 3). The extracellular region of mouse TLR2 is 89%, 67%, 81%, and 65% aa identical to the equivalent region in rat, human, hamster and canine, respectively.

 

References

  1. Wetzler, L. (2003) Vaccine 21:S2/55.
  2. Netea, M. et al. (2004) J. Leukoc. Biol. 75:749.
  3. Dunne, A. and L. O’Neill (2005) FEBS. Lett. 579:3330.
  4. Hopkins, P.A. and S. Sriskandan (2005) Clin. Exp. Immunol. 140:395.
  5. Matsuguchi, T. et al. (2000) Blood 95:1378.
  6. Meng, G. et al. (2005) Immunol. Lett. 98:200.
  7. Flo T. et al. (2001) J. Leukoc. Biol. 69:474.
  8. Schaefer, L. et al. (2005) J. Clin. Invest. 115:2223.
  9. Akira S. (2003) Curr. Opin. Immunol. 15:5.
  10. Hasan, U. et al. (2005) J. Immunol. 174:2942.
  11. Yamamoto M. et al. (2002) Nature 420:324.

Long Name

Toll-like Receptor 2

Alternate Names

CD282

Entrez Gene IDs

7097 (Human); 24088 (Mouse); 310553 (Rat)

Gene Symbol

TLR2

UniProt

Additional TLR2 Products

Product Documents for Recombinant Mouse TLR2 Fc Chimera Protein, CF

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Note: Certificate of Analysis not available for kit components.

Product Specific Notices for Recombinant Mouse TLR2 Fc Chimera Protein, CF

For research use only

Loading...
Loading...
Loading...