Recombinant Mouse TLR2 Fc Chimera Protein, CF

Name: Recombinant Mouse TLR2 Fc Chimera Protein, CF
Brand: R&D Systems
SKU: 1530-TR
Price: 417 USD

R&D Systems, Inc. a Bio-Techne Brand

Catalog # 1530-TR

Citations (6)

Product Datasheet / COA / SDS

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Key Product Details

Source:	Sf 21 (baculovirus)
Accession #:	Q9QUN7
Structure / Form:	Disulfide-linked homodimer
Applications:	Binding Activity

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Product Specifications for Recombinant Mouse TLR2 Fc Chimera Protein, CF

Source

Spodoptera frugiperda, Sf 21 (baculovirus)-derived mouse TLR2 protein

Mouse TLR2 (Gln25-Leu590) Accession # Q9QUN7	DIEGRMD	Human IgG₁ (Pro100-Lys330)
N-terminus		C-terminus

Purity

>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

Endotoxin level

<0.10 EU per 1 μg of the protein by the LAL method.

N-terminal sequence Analysis

No results obtained: Gln25 predicted

Predicted Molecular Mass

90.4 kDa (monomer)

SDS-PAGE

92 kDa, reducing conditions

Activity

Measured by its binding ability in a functional ELISA. Immobilized peptidoglycan at 10 µg/mL (100 µL/well) can bind recombinant mouse TLR2 Fc Chimera with a linear range of 0.04-30 µg/mL.

Formulation, Preparation and Storage

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

1530-TR

Formulation	Lyophilized from a 0.2 μm filtered solution in PBS, NaCl and PEG 3350.
Reconstitution	Reconstitute at 100 μg/mL in sterile PBS. Reconstitution Buffer Available: Size / Price Qty Loading... RB01:
Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage:	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: TLR2

The Toll-like family of molecules are a group of integral membrane proteins that serve as pattern recognition receptors for microbial pathogens (1 - 4). To date, there are at least eleven mouse and ten human members that activate the innate immune system following exposure to a variety of microbial species (1, 3). All Toll-like receptors (TLRs) are type I transmembrane (TM) proteins that exist either in the plasma membrane or in the membranes of endosomal structures (where they bind intracellular nucleic acids) (3). All TLRs also contain a large number of extracellular leucine-rich repeats (LRRs) and a cytoplasmic tail with a Toll/IL-1 receptor (TIR) domain. Mouse Toll-like receptor-2 (TLR2) is a 97 kDa, 760 amino acid (aa) glycoprotein that contains a 563 aa extracellular region, a 21 aa TM segment, and a 176 aa cytoplasmic domain (5, 6). The extracellular region contains 16 leucine-rich repeats, while the cytoplasmic tail shows one 146 aa TIR domain. The receptor is expressed on a number of cell types including T cells ( alpha beta and gamma δ), monocytes, dendritic cells, neutrophils, B cells, endothelial cells, mast cells, NK cells, macrophages and hepatocytes (1, 4, 5, 7, 8). TLR2 functions as part of a heterodimeric complex with either TLR1 or TLR6 (1, 3, 4). These complexes recognize lipoproteins and glycolipids from gram-positive and gram-negative bacteria as well as mycoplasma and yeast. TLR2/TLR1 heterodimers recognize triacylated lipopeptides from a variety of microorganisms. The TLR2/TLR6 heterodimer preferentially recognizes diacylated lipopeptides (9). Biglycan is also known to activate TLR2, but the context is unclear (8). Notably, in human, TLR2 also dimerizes with TLR10. But the TLR10 gene in mouse (but not rat) is mutationally inactive, and thus this complex is nonfunctional (10). Upon ligand recognition, TLR2 delivers an activating signal via the associated adapter molecules, MyD88 and TIRAP (1, 11). Activation via TLR2 also results in production of a number of pro-inflammatory cytokines including TNF-alpha, IL-2, IL-6, IL-12, and MIP-2 (1, 3). The extracellular region of mouse TLR2 is 89%, 67%, 81%, and 65% aa identical to the equivalent region in rat, human, hamster and canine, respectively.

Item	Value
References	Wetzler, L. (2003) Vaccine 21:S2/55. Netea, M. et al. (2004) J. Leukoc. Biol. 75:749. Dunne, A. and L. O’Neill (2005) FEBS. Lett. 579:3330. Hopkins, P.A. and S. Sriskandan (2005) Clin. Exp. Immunol. 140:395. Matsuguchi, T. et al. (2000) Blood 95:1378. Meng, G. et al. (2005) Immunol. Lett. 98:200. Flo T. et al. (2001) J. Leukoc. Biol. 69:474. Schaefer, L. et al. (2005) J. Clin. Invest. 115:2223. Akira S. (2003) Curr. Opin. Immunol. 15:5. Hasan, U. et al. (2005) J. Immunol. 174:2942. Yamamoto M. et al. (2002) Nature 420:324.
Long Name	Toll-like Receptor 2
Entrez Gene IDs	7097 (Human); 24088 (Mouse); 310553 (Rat)
Alternate Names	CD282, CD282 antigen, TIL4CD282, TLR2, Toll/interleukin-1 receptor-like protein 4, toll-like receptor 2, toll/interleukin 1 receptor-like 4

Citations for Recombinant Mouse TLR2 Fc Chimera Protein, CF (6)

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Citations are publications that use Bio-Techne products. Selected citations for Recombinant Mouse TLR2 Fc Chimera Protein, CF include:

Species: Human
Sample Types: Whole Cells
Applications: Bioassay

N Maehara et al. (2021-01-01), AIM/CD5L attenuates DAMPs in the injured brain and thereby ameliorates ischemic stroke Cell Reports, 36(11):109693.
PMID: 34525359
Species: Mouse
Sample Types: Cell Lysate
Applications: Western Blot Control

X Xie et al. (2021-01-01), HBeAg mediates inflammatory functions of macrophages by TLR2 contributing to hepatic fibrosis Bmc Medicine, 19(1):247.
PMID: 34649530
Species: Human
Sample Types: Protein
Applications: Bioassay

JA George et al. (2017-01-01), TLR2/MyD88 pathway-dependent regulation of dendritic cells by dengue virus promotes antibody-dependent enhancement via Th2-biased immunity Oncotarget, 8(62):106050-106070.
PMID: 29285314
Species: Bacteria - Staphylococcus aureus
Sample Types: Recombinant Protein
Applications: Bioassay

Koymans K et al. (2015-01-01), Structural basis for inhibition of TLR2 by staphylococcal superantigen-like protein 3 (SSL3). Proc Natl Acad Sci U S A, 112(35):11018-23.
PMID: 26283364
Species: Mouse
Sample Types: Recombinant Protein
Applications: ELISA (Capture)

Onishi S et al. (2007-01-01), Toll-like receptor 2-mediated interleukin-8 expression in gingival epithelial cells by the Tannerella forsythia leucine-rich repeat protein BspA. Infect. Immun., 76(1):198-205.
PMID: 17967853
Species: Staphylococcus epidermidis
Sample Types: Whole Cells
Applications: Bioassay

Raby A et al. (), Soluble TLR2 reduces inflammation without compromising bacterial clearance by disrupting TLR2 triggering. J Immuno183(1):506-17.
PMID: 19542461

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Showing 1 - 6 of 6 citations Showing All

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Recombinant Mouse TLR2 Fc Chimera Protein, CF

R&D Systems, Inc. a Bio-Techne Brand

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