Skip to main content

Recombinant Mouse TIM-4 Protein, CF

Catalog # 2826-TI | R&D Systems, Inc. a Bio-Techne Brand
Catalog #
Availability
Size / Price
Qty
Loading...
2826-TI-050

Key Product Details

Source

NS0

Accession #

Conjugate

Unconjugated

Applications

Bioactivity

Product Specifications

Source

Mouse myeloma cell line, NS0-derived mouse TIM-4 protein
Ala22-Thr279, with a C-terminal 6-His tag

Purity

>95%, by SDS-PAGE under reducing conditions and visualized by silver stain.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

Ala22

Predicted Molecular Mass

28.7 kDa

SDS-PAGE

70-80 kDa, reducing conditions

Activity

Measured by its ability to inhibit anti-CD3-induced proliferation of stimulated human T cells.
Human T lymphocytes cultured for 72 hours with PHA were incubated for an additional 3 days in 96 well plates coated with 500 ng/mL anti-CD3 and rmTIM-4.
The ED50 for this effect is 0.25-1 µg/mL.
Optimal dilutions should be determined by each laboratory for each application.

Scientific Data Images for Recombinant Mouse TIM-4 Protein, CF

Recombinant Mouse TIM-4 Protein Bioactivity

Recombinant Mouse TIM-4 Protein Bioactivity

Recombinant Mouse TIM-4 (Catalog # 2826-TI) inhibits anti-CD3-induced proliferation of stimulated human T cells. Human T cells cultured for 72 hours with PHA are incubated for an additional 3 days in 96 well plates coated with 500 ng/mL anti-CD3 and rmTIM-4. The ED50 for this effect is 0.25-1 µg/mL.

Formulation, Preparation and Storage

2826-TI
Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution
Reconstitute at 100 μg/mL in sterile PBS.

Reconstitution Buffer Available:
Size / Price
Qty
Loading...
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: TIM-4

TIM-4 (T cell; immunoglobulin; mucin-4), also known as SMUCKLER, is a 60 kDa member of the TIM family of immune regulating proteins. TIMs are type I transmembrane proteins with one Ig-like V domain and one Ser/Thr-rich mucin domain (1 - 3). The mouse TIM-4 cDNA encodes a 343 amino acid (aa) precursor that includes a 22 aa signal sequence, a 257 aa extracellular domain (ECD), a 21 aa transmembrane segment, and a 43 aa cytoplasmic tail (4). Structurally, TIM-4 is distinguished from other TIMs by the presence of an RGD motif in its Ig domain and the lack of a site for tyrosine phosphorylation in its cytoplasmic tail. The mucin domain in TIM-4 is larger than in TIM-1, -2, or -3. Within the ECD, mouse TIM-4 shares 27 - 33% aa sequence identity with mouse TIM-1, -2, and -3. The ECD of mouse TIM-4 shares 45% aa sequence identity with that of human and rat TIM-4. TIM-4 is expressed by macrophages and mature dendritic cells but not by lymphocytes (4, 5). TIM-4 binds specifically to TIM-1 which is also the cellular receptor for hepatitis A virus and has been implicated in the development of asthma (5 - 7). Among hematopoietic cells, TIM-1 is expressed on activated B and T cells, preferentially in the Th2 subset of CD4+ T cells (5, 8). The interaction ofTIM-4 with TIM-1 induces co-stimulatory and hyperproliferative signals in T cells (5).

References

  1. Kuchroo, V.K. et al. (2003) Nat. Rev. Immunol. 3:454. 
  2. Mariat, C. et al. (2005) Phil. Trans. R. Soc. B 360:1681.  
  3. Meyers, J.H. et al. (2005) Trends Mol. Med. 11:362. 
  4. Shakhov, A.N. et al. (2004) Eur. J. Immunol. 34:494. 
  5. Meyers, J.H. et al. (2005) Nat. Immunol. 6:455.
  6. Feigelstock, D. et al. (1998) J. Virol. 72:6621.
  7. McIntire, J.J. et al. (2001) Nat. Immunol. 2:1109.
  8. Khademi, M. et al. (2004) J. Immunol. 172:7169.

Long Name

T Cell Immunoglobulin Mucin-4

Alternate Names

Smuckler, TIM4, TIMD4

Entrez Gene IDs

91937 (Human); 276891 (Mouse); 102140672 (Cynomolgus Monkey)

Gene Symbol

TIMD4

UniProt

Product Documents for Recombinant Mouse TIM-4 Protein, CF

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Note: Certificate of Analysis not available for kit components.

Product Specific Notices for Recombinant Mouse TIM-4 Protein, CF

For research use only

Citations for Recombinant Mouse TIM-4 Protein, CF (1)

Showing  1 - 1 of 1 publication Showing All
Filter By:
Search Title:

Citations are publications that use Bio-Techne products. Selected citations for Recombinant Mouse TIM-4 Protein, CF include:

There are no citations that match your criteria.

Showing  1 - 1 of 1 publication Showing All

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

÷
=
Loading...
Setting the standard in quality research reagents