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Recombinant Mouse TEM8/ANTXR1 Fc Chimera Protein, CF

Catalog # 10202-AR | R&D Systems, Inc. a Bio-Techne Brand
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10202-AR-050

Key Product Details

Source

NS0

Accession #

Structure / Form

Disulfide-linked homodimer

Conjugate

Unconjugated

Applications

Bioactivity

Product Specifications

Source

Mouse myeloma cell line, NS0-derived mouse TEM8/ANTXR1 protein
Mouse TEM8/ANTXR1
(Glu31-Ser319)
Accession # Q9CZ52
IEGRMDP Mouse IgG2a
(Glu98-Lys330)
N-terminus C-terminus

Purity

>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

Glu31

Predicted Molecular Mass

60 kDa

SDS-PAGE

62-81 kDa, under reducing conditions

Activity

Measured by its binding ability in a functional ELISA.
When Anthrax Protective Antigen is immobilized at 1.5 μg/mL (100 μL/well), the concentration of Recombinant Mouse TEM8/ANTXR1 Fc Chimera (Catalog # 10202-AR) that produces 50% of the optimal binding response is 5-30 ng/mL.

Scientific Data Images for Recombinant Mouse TEM8/ANTXR1 Fc Chimera Protein, CF

Recombinant Mouse TEM8/ANTXR1 Fc Chimera Protein Binding Activity

Recombinant Mouse TEM8/ANTXR1 Fc Chimera Protein Binding Activity

When Anthrax Protective Antigen is immobilized at 1.5 μg/mL, 100 μL/well, Recombinant Mouse TEM8/ANTXR1 Fc Chimera (Catalog # 10202-AR) binds with an ED50 of 5-30 ng/mL.
Recombinant Mouse TEM8/ANTXR1 Fc Chimera Protein SDS-PAGE

Recombinant Mouse TEM8/ANTXR1 Fc Chimera Protein SDS-PAGE

2 μg/lane of Recombinant Mouse TEM8/ANTXR1 Fc Chimera (Catalog # 10202-AR) was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by Coomassie® Blue staining, showing bands at 62-81 kDa and 125-160 kDa, respectively.

Formulation, Preparation and Storage

10202-AR
Formulation Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.
Reconstitution Reconstitute at 500 μg/mL in PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: TEM8/ANTXR1

Anthrax toxin receptor 1 (ANTXR1), also known as Tumor endothelial marker 8 (TEM8), is a glycoprotein of the Anthrax Toxin Receptor family that is expressed by endothelial cells. TEM8/ANTXR1 contains a 289 amino acid (aa) extracellular domain, a 21 aa transmembrane domain, and a 222 aa cytoplasmic domain. Isoforms diverging at the C-termiuns include 564 aa (80‑85 kDa), 368 aa (60 kDa), and potentially secreted isoforms of 330 aa and 297 aa (45 kDa) (1). The extracellular domain shares structural similarity with von Willebrand factor type (vWFA) domains, which are characterized by their interactions with ECM components (2, 3). The extracellular domain is involved in reorganization of cell actin cytoskeleton (2, 3). TEM8/ANTXR1 binds Anthrax Protective Antigen with lesser affinity that Anthrax Receptor 2 and induces toxin internalization (4). TEM8/ANTXR1 has been implicated in tumor angiogenesis, as its expression has been shown to up-regulate in tumor blood vessels and is characterized as a tumor endothelial marker (5). TEM8/ANTXR1 was reported to be an amplifier of Wnt signaling in tumor microenvironment (6). Additionally, TEM8/ANTXR1 serves as the receptor for Seneca Valley virus, an oncolytic picornavirus affecting neuroendocrine cancers (7). Mouse TEM8/ANTXR1 shares 99% and 100% aa identity with human and rat TEM8/ANTXR1, respectively, within the extracellular domain.

References

  1. Bradley, KA. et al. (2001) Nature 414:225.
  2. Hotchkiss, K. et al. (2004). Experimental Cell Research. 305:133.
  3. Whittaker, CA. and Hynes, R. (2002). Mol Biol Cell. 13:3369.
  4. Fu, S. et al. (2010) PLOS One. 5:e11203.
  5. Carson-Walter, EB. et al. (2001). Cancer Res. 18:6649.
  6. Verma, K. et al. (2011) PLOS One. 6:e22334.
  7. Miles, L. et al. (2017). J Clin Invest. 8:2957.

Long Name

Tumor Endothelial Marker 8/Anthrax Toxin Receptor 1

Alternate Names

ANTXR1

Entrez Gene IDs

84168 (Human); 69538 (Mouse); 362393 (Rat)

Gene Symbol

ANTXR1

UniProt

Product Documents for Recombinant Mouse TEM8/ANTXR1 Fc Chimera Protein, CF

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

Product Specific Notices for Recombinant Mouse TEM8/ANTXR1 Fc Chimera Protein, CF

For research use only

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