Recombinant Mouse TEM8/ANTXR1 Fc Chimera Protein, CF

Name: Recombinant Mouse TEM8/ANTXR1 Fc Chimera Protein, CF
Brand: R&D Systems
SKU: 10202-AR

Catalog # 10202-AR

Product Datasheet / COA / SDS

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Key Product Details

Source:	NS0
Accession #:	Q9CZ52
Structure / Form:	Disulfide-linked homodimer
Applications:	Bioactivity

View Full Technical Details for Recombinant Mouse TEM8/ANTXR1 Fc Chimera Protein, CF

Product Specifications

Source

Mouse myeloma cell line, NS0-derived mouse TEM8/ANTXR1 protein

Mouse TEM8/ANTXR1 (Glu31-Ser319) Accession # Q9CZ52	IEGRMDP	Mouse IgG_2a (Glu98-Lys330)
N-terminus		C-terminus

Purity

>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

Endotoxin level

<0.10 EU per 1 μg of the protein by the LAL method.

N-terminal sequence Analysis

Glu31

Predicted Molecular Mass

60 kDa

SDS-PAGE

62-81 kDa, under reducing conditions

Activity

Measured by its binding ability in a functional ELISA. When Anthrax Protective Antigen is immobilized at 1.5 μg/mL (100 μL/well), the concentration of Recombinant Mouse TEM8/ANTXR1 Fc Chimera (Catalog # 10202-AR) that produces 50% of the optimal binding response is 5-30 ng/mL.

Recombinant Mouse TEM8/ANTXR1 Fc Chimera Protein, CF Scientific Data Examples

	Recombinant Mouse TEM8/ANTXR1 Fc Chimera Protein Binding Activity When Anthrax Protective Antigen is immobilized at 1.5 μg/mL, 100 μL/well, Recombinant Mouse TEM8/ANTXR1 Fc Chimera (Catalog # 10202-AR) binds with an ED₅₀ of 5-30 ng/mL.
	Recombinant Mouse TEM8/ANTXR1 Fc Chimera Protein SDS-PAGE 2 μg/lane of Recombinant Mouse TEM8/ANTXR1 Fc Chimera (Catalog # 10202-AR) was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by Coomassie® Blue staining, showing bands at 62-81 kDa and 125-160 kDa, respectively.

Formulation, Preparation and Storage

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

10202-AR

Formulation	Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.
Reconstitution	Reconstitute at 500 μg/mL in PBS.
Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage:	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

Volume (to add to vial)

Mass (in vial)

Desired Reconstitution Concentration

Background: TEM8/ANTXR1

Anthrax toxin receptor 1 (ANTXR1), also known as Tumor endothelial marker 8 (TEM8), is a glycoprotein of the Anthrax Toxin Receptor family that is expressed by endothelial cells. TEM8/ANTXR1 contains a 289 amino acid (aa) extracellular domain, a 21 aa transmembrane domain, and a 222 aa cytoplasmic domain. Isoforms diverging at the C-termiuns include 564 aa (80‑85 kDa), 368 aa (60 kDa), and potentially secreted isoforms of 330 aa and 297 aa (45 kDa) (1). The extracellular domain shares structural similarity with von Willebrand factor type (vWFA) domains, which are characterized by their interactions with ECM components (2, 3). The extracellular domain is involved in reorganization of cell actin cytoskeleton (2, 3). TEM8/ANTXR1 binds Anthrax Protective Antigen with lesser affinity that Anthrax Receptor 2 and induces toxin internalization (4). TEM8/ANTXR1 has been implicated in tumor angiogenesis, as its expression has been shown to up-regulate in tumor blood vessels and is characterized as a tumor endothelial marker (5). TEM8/ANTXR1 was reported to be an amplifier of Wnt signaling in tumor microenvironment (6). Additionally, TEM8/ANTXR1 serves as the receptor for Seneca Valley virus, an oncolytic picornavirus affecting neuroendocrine cancers (7). Mouse TEM8/ANTXR1 shares 99% and 100% aa identity with human and rat TEM8/ANTXR1, respectively, within the extracellular domain.

Item	Value
References	Bradley, KA. et al. (2001) Nature 414:225. Hotchkiss, K. et al. (2004). Experimental Cell Research. 305:133. Whittaker, CA. and Hynes, R. (2002). Mol Biol Cell. 13:3369. Fu, S. et al. (2010) PLOS One. 5:e11203. Carson-Walter, EB. et al. (2001). Cancer Res. 18:6649. Verma, K. et al. (2011) PLOS One. 6:e22334. Miles, L. et al. (2017). J Clin Invest. 8:2957.
Long Name	Tumor Endothelial Marker 8/Anthrax Toxin Receptor 1
Entrez Gene IDs	84168 (Human); 69538 (Mouse); 362393 (Rat)
Alternate Names	ANTXR1, ATR2310008J16Rik, FLJ10601, FLJ11298, FLJ21776, TEM8, TEM82810405N18Rik, Tumor endothelial marker 8, anthrax toxin receptor 1

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