Recombinant Mouse TACI/TNFRSF13B Fc Chimera Protein, CF

Name: Recombinant Mouse TACI/TNFRSF13B Fc Chimera Protein, CF
Brand: R&D Systems
SKU: 1041-TC

Catalog # 1041-TC

Citations (4)

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Key Product Details

Source:	NS0
Accession #:	Q9ET35
Structure / Form:	Disulfide-linked homodimer
Applications:	Bioactivity

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Product Specifications

Source

Mouse myeloma cell line, NS0-derived mouse TACI/TNFRSF13B protein

Mouse TACI (Phe5 - Thr129) Accession # Q9ET35	IEGRMD	Human IgG₁ (Pro100 - Lys330)
N-terminus		C-terminus

Purity

>90%, by SDS-PAGE under reducing conditions and visualized by silver stain

Endotoxin level

<0.10 EU per 1 μg of the protein by the LAL method.

N-terminal sequence Analysis

Phe5

Predicted Molecular Mass

41 kDa (monomer)

SDS-PAGE

47 kDa, reducing conditions

Activity

Measured by its ability to inhibit BAFF-mediated proliferation of anti-IgM stimulated mouse B cells. Moore, P.A. et al. (1999) Science 285:260; Gross, J.A. et al. (2000) Nature 404:995; Schneider, P. et al. (1999) J. Exp. Med. 189:1747. The ED₅₀ for this effect is 3‑10 ng/mL in the presence of 2.5 ng/mL of rhBAFF.

Formulation, Preparation and Storage

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

1041-TC

Formulation	Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution	Reconstitute at 100 μg/mL in sterile PBS. Reconstitution Buffer Available: Size / Price Qty Loading... RB01:
Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage:	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: TACI/TNFRSF13B

TACI, transmembrane activator and CAML-interactor, is a member of the TNF receptor superfamily and has been designated TNFRSF13B. TACI is a type III membrane protein with an extracellular N-terminus in the absence of a cleaved signal sequence. The extracellular region of TACI contains two cysteine-rich domains. Within the TNFRSF, it shares the highest homology with B cell maturation factor (BCMA). TACI and BCMA have both been shown to bind APRIL and BAFF, members of the TNF ligand superfamily. TACI is expressed on the cell surface of B cells and activated, but not resting, T cells. Analogous to BCMA, data suggests that TACI may play an important role in B cell development, function and regulation. Mouse TACI is a 249 amino acid (aa) protein consisting of a 129 aa extracellular domain, a 20 aa transmembrane domain, and a 100 aa intracellular domain. Mouse and human TACI share 54% aa identity.

Item	Value
References	Xia, X.Z. et al. (2000) J. Exp. Med. 192:137. von Bulow, G.U. et al. (1997) Science 278:138. Gross, J.A. et al. (2000) Nature 404:995. Marsters, S.A. et al. (2000) Curr. Biol. 10:785. Yan, M. et al. (2000) Nature Immunol. 1:37. Wu, Y. et al. (2000) J. Biol. Chem. 275:35478.
Long Name	Transmembrane Activator and CAML Interactor/Tumor Necrosis Factor Receptor Superfamily Member 13B
Entrez Gene IDs	23495 (Human); 57916 (Mouse); 102126451 (Cynomolgus Monkey)
Alternate Names	CD267, CD267 antigen, CVID, CVID2, FLJ39942, MGC133214, TACI, TACIMGC39952, TNFRSF13B, TNFRSF14B, Transmembrane activator and CAML interactor, tumor necrosis factor receptor 13B, tumor necrosis factor receptor superfamily member 13B, tumor necrosis factor receptor superfamily, member 13B

Citations for Recombinant Mouse TACI/TNFRSF13B Fc Chimera Protein, CF (4)

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Citations are publications that use Bio-Techne products. Selected citations for Recombinant Mouse TACI/TNFRSF13B Fc Chimera Protein, CF include:

Species: Mouse
Sample Types: Whole Cells
Applications: Bioassay

Hoffmann F et al. (2015), The immunoregulator soluble TACI is released by ADAM10 and reflects B cell activation in autoimmunity. J Immunol, 194(2):542-52.
PMID: 25505277
Species: Mouse
Sample Types: Whole Cells
Applications: Bioassay

Kotani Y et al. (2014), Role of Lactobacillus pentosus Strain b240 and the Toll-like receptor 2 axis in Peyer's patch dendritic cell-mediated immunoglobulin A enhancement. PLoS ONE, 9(3):e91857.
PMID: 24632732
Species: Mouse
Sample Types: Recombinant Protein
Applications: Binding Assay

Kimberley et al. (2012), The Design and Characterization of Receptor-selective APRIL Variants. J Biol Chem, 287(44):37434-46.
PMID: 22961987
Species: Mouse
Sample Types: Whole Cells
Applications: Bioassay

Suzuki K et al. (2010), The sensing of environmental stimuli by follicular dendritic cells promotes immunoglobulin A generation in the gut. Immunity, 33(1):71-83.
PMID: 20643338

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